Experiment: formate McAA folic dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08670 and MMP_RS08675 are separated by 19 nucleotides MMP_RS08675 and MMP_RS08680 are separated by 137 nucleotides MMP_RS08680 and MMP_RS08685 are separated by 108 nucleotides MMP_RS08685 and MMP_RS08690 are separated by 16 nucleotides MMP_RS08690 and MMP_RS08695 are separated by 17 nucleotides MMP_RS08695 and MMP_RS08700 are separated by 10 nucleotides MMP_RS08700 and MMP_RS08705 are separated by 12 nucleotides
MMP_RS08670: MMP_RS08670 - metallophosphoesterase, at 1,626,448 to 1,627,131
_RS08670
MMP_RS08675: MMP_RS08675 - class III signal peptide-containing protein, at 1,627,151 to 1,627,375
_RS08675
MMP_RS08680: MMP_RS08680 - tRNA-Trp, at 1,627,513 to 1,627,676
_RS08680
MMP_RS08685: MMP_RS08685 - hypothetical protein, at 1,627,785 to 1,627,982
_RS08685
MMP_RS08690: MMP_RS08690 - RNA-guided pseudouridylation complex pseudouridine synthase subunit Cbf5, at 1,627,999 to 1,629,000
_RS08690
MMP_RS08695: MMP_RS08695 - ferredoxin family protein, at 1,629,018 to 1,629,224
_RS08695
MMP_RS08700: MMP_RS08700 - hypothetical protein, at 1,629,235 to 1,629,918
_RS08700
MMP_RS08705: MMP_RS08705 - sulfopyruvate decarboxylase subunit beta, at 1,629,931 to 1,630,488
_RS08705
Position (kb)
1627
1628
1629
1630 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1627.015 kb on + strand, within MMP_RS08670 at 1627.270 kb on + strand, within MMP_RS08675 at 1627.335 kb on + strand, within MMP_RS08675 at 1627.335 kb on + strand, within MMP_RS08675 at 1627.378 kb on + strand at 1627.378 kb on + strand at 1627.428 kb on + strand at 1627.429 kb on - strand at 1627.430 kb on + strand at 1627.430 kb on + strand at 1627.433 kb on - strand at 1627.437 kb on - strand at 1627.736 kb on + strand at 1627.755 kb on - strand at 1627.756 kb on + strand at 1627.768 kb on - strand at 1627.768 kb on - strand at 1627.769 kb on + strand at 1627.771 kb on + strand at 1627.771 kb on + strand at 1627.808 kb on + strand, within MMP_RS08685 at 1627.834 kb on - strand, within MMP_RS08685 at 1627.838 kb on + strand, within MMP_RS08685 at 1627.843 kb on + strand, within MMP_RS08685 at 1627.843 kb on + strand, within MMP_RS08685 at 1627.843 kb on + strand, within MMP_RS08685 at 1627.844 kb on - strand, within MMP_RS08685 at 1627.845 kb on + strand, within MMP_RS08685 at 1627.847 kb on + strand, within MMP_RS08685 at 1627.850 kb on - strand, within MMP_RS08685 at 1627.860 kb on - strand, within MMP_RS08685 at 1627.860 kb on - strand, within MMP_RS08685 at 1627.861 kb on + strand, within MMP_RS08685 at 1627.862 kb on - strand, within MMP_RS08685 at 1627.862 kb on - strand, within MMP_RS08685 at 1627.862 kb on - strand, within MMP_RS08685 at 1627.863 kb on + strand, within MMP_RS08685 at 1627.863 kb on + strand, within MMP_RS08685 at 1627.863 kb on + strand, within MMP_RS08685 at 1627.865 kb on - strand, within MMP_RS08685 at 1627.865 kb on - strand, within MMP_RS08685 at 1627.880 kb on + strand, within MMP_RS08685 at 1627.880 kb on + strand, within MMP_RS08685 at 1627.909 kb on - strand, within MMP_RS08685 at 1627.910 kb on + strand, within MMP_RS08685 at 1628.013 kb on + strand at 1628.037 kb on - strand at 1628.037 kb on - strand at 1628.056 kb on - strand at 1628.279 kb on + strand, within MMP_RS08690 at 1628.456 kb on - strand, within MMP_RS08690 at 1628.456 kb on - strand, within MMP_RS08690 at 1628.457 kb on + strand, within MMP_RS08690 at 1628.486 kb on + strand, within MMP_RS08690 at 1628.489 kb on - strand, within MMP_RS08690 at 1628.618 kb on + strand, within MMP_RS08690 at 1628.619 kb on - strand, within MMP_RS08690 at 1628.620 kb on + strand, within MMP_RS08690 at 1628.622 kb on + strand, within MMP_RS08690 at 1628.712 kb on + strand, within MMP_RS08690 at 1628.872 kb on + strand, within MMP_RS08690 at 1628.876 kb on - strand, within MMP_RS08690 at 1628.918 kb on - strand at 1628.961 kb on - strand at 1628.988 kb on + strand at 1629.125 kb on + strand at 1629.442 kb on - strand, within MMP_RS08700 at 1629.490 kb on - strand, within MMP_RS08700 at 1629.491 kb on + strand, within MMP_RS08700 at 1629.495 kb on + strand, within MMP_RS08700 at 1629.532 kb on + strand, within MMP_RS08700 at 1629.559 kb on - strand, within MMP_RS08700 at 1629.561 kb on - strand, within MMP_RS08700 at 1629.691 kb on - strand, within MMP_RS08700 at 1629.691 kb on - strand, within MMP_RS08700 at 1629.692 kb on + strand, within MMP_RS08700 at 1629.701 kb on + strand, within MMP_RS08700 at 1629.864 kb on + strand at 1629.917 kb on + strand at 1629.965 kb on + strand at 1629.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA folic dropout remove 1,627,015 + MMP_RS08670 0.83 +0.1 1,627,270 + MMP_RS08675 0.53 -0.5 1,627,335 + MMP_RS08675 0.82 -0.3 1,627,335 + MMP_RS08675 0.82 -0.2 1,627,378 + +0.4 1,627,378 + -0.9 1,627,428 + +0.3 1,627,429 - +0.0 1,627,430 + -0.1 1,627,430 + -0.2 1,627,433 - -0.1 1,627,437 - +0.4 1,627,736 + -1.0 1,627,755 - +0.5 1,627,756 + +0.3 1,627,768 - +0.7 1,627,768 - +0.0 1,627,769 + +0.2 1,627,771 + -0.3 1,627,771 + -0.7 1,627,808 + MMP_RS08685 0.12 -0.7 1,627,834 - MMP_RS08685 0.25 +0.2 1,627,838 + MMP_RS08685 0.27 -0.2 1,627,843 + MMP_RS08685 0.29 +0.6 1,627,843 + MMP_RS08685 0.29 -0.2 1,627,843 + MMP_RS08685 0.29 +0.2 1,627,844 - MMP_RS08685 0.30 -0.3 1,627,845 + MMP_RS08685 0.30 -0.1 1,627,847 + MMP_RS08685 0.31 +0.2 1,627,850 - MMP_RS08685 0.33 -0.9 1,627,860 - MMP_RS08685 0.38 +0.1 1,627,860 - MMP_RS08685 0.38 -0.6 1,627,861 + MMP_RS08685 0.38 -0.8 1,627,862 - MMP_RS08685 0.39 +0.2 1,627,862 - MMP_RS08685 0.39 +0.3 1,627,862 - MMP_RS08685 0.39 -0.6 1,627,863 + MMP_RS08685 0.39 -0.1 1,627,863 + MMP_RS08685 0.39 -0.2 1,627,863 + MMP_RS08685 0.39 -0.6 1,627,865 - MMP_RS08685 0.40 -0.8 1,627,865 - MMP_RS08685 0.40 -0.7 1,627,880 + MMP_RS08685 0.48 -0.2 1,627,880 + MMP_RS08685 0.48 -0.4 1,627,909 - MMP_RS08685 0.63 +0.3 1,627,910 + MMP_RS08685 0.63 -0.2 1,628,013 + -0.6 1,628,037 - -0.5 1,628,037 - +0.2 1,628,056 - -1.2 1,628,279 + MMP_RS08690 0.28 -1.0 1,628,456 - MMP_RS08690 0.46 -0.7 1,628,456 - MMP_RS08690 0.46 +0.5 1,628,457 + MMP_RS08690 0.46 -0.7 1,628,486 + MMP_RS08690 0.49 -0.6 1,628,489 - MMP_RS08690 0.49 -0.4 1,628,618 + MMP_RS08690 0.62 -1.1 1,628,619 - MMP_RS08690 0.62 -1.8 1,628,620 + MMP_RS08690 0.62 -0.5 1,628,622 + MMP_RS08690 0.62 -1.0 1,628,712 + MMP_RS08690 0.71 -1.7 1,628,872 + MMP_RS08690 0.87 -0.6 1,628,876 - MMP_RS08690 0.88 -0.5 1,628,918 - -0.5 1,628,961 - -1.7 1,628,988 + -0.9 1,629,125 + -3.3 1,629,442 - MMP_RS08700 0.30 +0.4 1,629,490 - MMP_RS08700 0.37 -0.8 1,629,491 + MMP_RS08700 0.37 -0.1 1,629,495 + MMP_RS08700 0.38 +0.1 1,629,532 + MMP_RS08700 0.43 +0.4 1,629,559 - MMP_RS08700 0.47 +0.3 1,629,561 - MMP_RS08700 0.48 +0.2 1,629,691 - MMP_RS08700 0.67 -1.2 1,629,691 - MMP_RS08700 0.67 +0.1 1,629,692 + MMP_RS08700 0.67 +0.3 1,629,701 + MMP_RS08700 0.68 +0.7 1,629,864 + +0.2 1,629,917 + -0.3 1,629,965 + +0.5 1,629,979 - +0.5
Or see this region's nucleotide sequence