Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05631 and H281DRAFT_05632 are separated by 88 nucleotides H281DRAFT_05632 and H281DRAFT_05633 are separated by 85 nucleotides H281DRAFT_05633 and H281DRAFT_05634 are separated by 209 nucleotides
H281DRAFT_05631: H281DRAFT_05631 - two component transcriptional regulator, winged helix family, at 56,344 to 57,087
_05631
H281DRAFT_05632: H281DRAFT_05632 - hypothetical protein, at 57,176 to 57,823
_05632
H281DRAFT_05633: H281DRAFT_05633 - hypothetical protein, at 57,909 to 58,370
_05633
H281DRAFT_05634: H281DRAFT_05634 - NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family, at 58,580 to 59,440
_05634
Position (kb) 57
58
59 Strain fitness (log2 ratio) -1
0
1
2 at 57.026 kb on + strand at 57.027 kb on - strand at 57.046 kb on + strand at 57.047 kb on - strand at 57.100 kb on - strand at 57.155 kb on - strand at 57.155 kb on - strand at 57.375 kb on + strand, within H281DRAFT_05632 at 57.376 kb on - strand, within H281DRAFT_05632 at 57.615 kb on + strand, within H281DRAFT_05632 at 58.013 kb on + strand, within H281DRAFT_05633 at 58.126 kb on + strand, within H281DRAFT_05633 at 58.194 kb on - strand at 58.387 kb on - strand at 58.576 kb on + strand at 58.768 kb on + strand, within H281DRAFT_05634 at 58.770 kb on + strand, within H281DRAFT_05634 at 58.770 kb on + strand, within H281DRAFT_05634 at 58.771 kb on - strand, within H281DRAFT_05634
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 57,026 + +0.4 57,027 - +0.5 57,046 + +0.4 57,047 - -0.3 57,100 - -0.2 57,155 - +0.1 57,155 - +1.4 57,375 + H281DRAFT_05632 0.31 +0.1 57,376 - H281DRAFT_05632 0.31 -1.5 57,615 + H281DRAFT_05632 0.68 -0.0 58,013 + H281DRAFT_05633 0.23 +0.8 58,126 + H281DRAFT_05633 0.47 +0.4 58,194 - +2.1 58,387 - +0.1 58,576 + -1.2 58,768 + H281DRAFT_05634 0.22 +0.1 58,770 + H281DRAFT_05634 0.22 -0.5 58,770 + H281DRAFT_05634 0.22 +0.2 58,771 - H281DRAFT_05634 0.22 +0.2
Or see this region's nucleotide sequence