Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04398 and H281DRAFT_04399 are separated by 19 nucleotides H281DRAFT_04399 and H281DRAFT_04400 are separated by 156 nucleotides H281DRAFT_04400 and H281DRAFT_04401 are separated by 201 nucleotides
H281DRAFT_04398: H281DRAFT_04398 - Methylase involved in ubiquinone/menaquinone biosynthesis, at 112,775 to 113,680
_04398
H281DRAFT_04399: H281DRAFT_04399 - hydroxyacylglutathione hydrolase, at 113,700 to 114,491
_04399
H281DRAFT_04400: H281DRAFT_04400 - membrane-bound lytic murein transglycosylase D, at 114,648 to 116,297
_04400
H281DRAFT_04401: H281DRAFT_04401 - Cyanate permease, at 116,499 to 117,737
_04401
Position (kb)
114
115
116
117 Strain fitness (log2 ratio)
-1
0
1 at 113.660 kb on + strand at 113.661 kb on - strand at 113.661 kb on - strand at 113.681 kb on - strand at 113.716 kb on - strand at 113.720 kb on - strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.745 kb on + strand at 113.746 kb on - strand at 113.746 kb on - strand at 113.746 kb on - strand at 113.818 kb on - strand, within H281DRAFT_04399 at 113.854 kb on + strand, within H281DRAFT_04399 at 113.926 kb on - strand, within H281DRAFT_04399 at 114.020 kb on - strand, within H281DRAFT_04399 at 114.057 kb on + strand, within H281DRAFT_04399 at 114.226 kb on - strand, within H281DRAFT_04399 at 114.433 kb on + strand at 114.434 kb on - strand at 114.434 kb on - strand at 114.455 kb on + strand at 114.455 kb on + strand at 114.455 kb on + strand at 114.455 kb on + strand at 114.455 kb on + strand at 114.456 kb on - strand at 114.586 kb on + strand at 114.656 kb on + strand at 114.656 kb on + strand at 114.656 kb on + strand at 114.657 kb on - strand at 114.979 kb on - strand, within H281DRAFT_04400 at 114.984 kb on + strand, within H281DRAFT_04400 at 115.016 kb on + strand at 115.059 kb on + strand, within H281DRAFT_04400 at 115.061 kb on + strand, within H281DRAFT_04400 at 115.131 kb on + strand, within H281DRAFT_04400 at 115.132 kb on - strand, within H281DRAFT_04400 at 115.132 kb on - strand, within H281DRAFT_04400 at 115.149 kb on - strand, within H281DRAFT_04400 at 115.203 kb on + strand, within H281DRAFT_04400 at 115.204 kb on - strand, within H281DRAFT_04400 at 115.204 kb on - strand, within H281DRAFT_04400 at 115.326 kb on + strand, within H281DRAFT_04400 at 115.326 kb on + strand, within H281DRAFT_04400 at 115.327 kb on - strand, within H281DRAFT_04400 at 115.327 kb on - strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.330 kb on + strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.331 kb on - strand, within H281DRAFT_04400 at 115.362 kb on + strand, within H281DRAFT_04400 at 115.362 kb on + strand, within H281DRAFT_04400 at 115.362 kb on + strand, within H281DRAFT_04400 at 115.362 kb on + strand, within H281DRAFT_04400 at 115.363 kb on - strand, within H281DRAFT_04400 at 115.413 kb on + strand, within H281DRAFT_04400 at 115.450 kb on - strand, within H281DRAFT_04400 at 115.509 kb on - strand, within H281DRAFT_04400 at 115.575 kb on - strand, within H281DRAFT_04400 at 115.668 kb on + strand, within H281DRAFT_04400 at 115.668 kb on + strand, within H281DRAFT_04400 at 115.668 kb on + strand, within H281DRAFT_04400 at 115.669 kb on - strand, within H281DRAFT_04400 at 115.669 kb on - strand, within H281DRAFT_04400 at 116.295 kb on + strand at 116.295 kb on + strand at 116.296 kb on - strand at 116.296 kb on - strand at 116.373 kb on - strand at 116.498 kb on + strand at 116.498 kb on + strand at 116.499 kb on - strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.556 kb on + strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.557 kb on - strand at 116.594 kb on + strand at 116.690 kb on - strand, within H281DRAFT_04401 at 116.690 kb on - strand, within H281DRAFT_04401 at 116.692 kb on + strand, within H281DRAFT_04401 at 116.692 kb on + strand, within H281DRAFT_04401 at 116.692 kb on + strand, within H281DRAFT_04401 at 116.693 kb on - strand, within H281DRAFT_04401 at 117.107 kb on + strand, within H281DRAFT_04401 at 117.112 kb on + strand, within H281DRAFT_04401 at 117.112 kb on + strand, within H281DRAFT_04401 at 117.112 kb on + strand, within H281DRAFT_04401 at 117.112 kb on + strand, within H281DRAFT_04401 at 117.171 kb on + strand, within H281DRAFT_04401 at 117.171 kb on + strand, within H281DRAFT_04401
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 113,660 + -0.5 113,661 - -0.4 113,661 - -0.5 113,681 - +0.5 113,716 - +0.7 113,720 - -0.2 113,745 + -0.0 113,745 + -0.4 113,745 + -0.6 113,745 + -0.4 113,745 + +0.3 113,745 + +0.5 113,746 - +0.0 113,746 - +0.6 113,746 - +0.3 113,818 - H281DRAFT_04399 0.15 -0.3 113,854 + H281DRAFT_04399 0.19 +0.4 113,926 - H281DRAFT_04399 0.29 +0.7 114,020 - H281DRAFT_04399 0.40 +0.3 114,057 + H281DRAFT_04399 0.45 -0.4 114,226 - H281DRAFT_04399 0.66 -0.8 114,433 + +0.2 114,434 - +0.5 114,434 - +0.4 114,455 + +1.2 114,455 + +0.0 114,455 + +0.4 114,455 + -0.2 114,455 + +0.2 114,456 - -0.4 114,586 + +1.3 114,656 + +0.4 114,656 + +0.1 114,656 + +0.2 114,657 - -0.3 114,979 - H281DRAFT_04400 0.20 +0.4 114,984 + H281DRAFT_04400 0.20 +1.0 115,016 + +1.5 115,059 + H281DRAFT_04400 0.25 +0.3 115,061 + H281DRAFT_04400 0.25 +0.3 115,131 + H281DRAFT_04400 0.29 +1.1 115,132 - H281DRAFT_04400 0.29 +0.7 115,132 - H281DRAFT_04400 0.29 +0.6 115,149 - H281DRAFT_04400 0.30 -0.2 115,203 + H281DRAFT_04400 0.34 +0.1 115,204 - H281DRAFT_04400 0.34 +0.3 115,204 - H281DRAFT_04400 0.34 -0.0 115,326 + H281DRAFT_04400 0.41 +0.6 115,326 + H281DRAFT_04400 0.41 +0.7 115,327 - H281DRAFT_04400 0.41 +0.5 115,327 - H281DRAFT_04400 0.41 +1.0 115,330 + H281DRAFT_04400 0.41 +0.6 115,330 + H281DRAFT_04400 0.41 +0.5 115,330 + H281DRAFT_04400 0.41 +0.6 115,330 + H281DRAFT_04400 0.41 +1.5 115,330 + H281DRAFT_04400 0.41 +0.5 115,331 - H281DRAFT_04400 0.41 +0.4 115,331 - H281DRAFT_04400 0.41 +0.3 115,331 - H281DRAFT_04400 0.41 +0.7 115,331 - H281DRAFT_04400 0.41 +1.0 115,331 - H281DRAFT_04400 0.41 +1.1 115,331 - H281DRAFT_04400 0.41 +0.6 115,331 - H281DRAFT_04400 0.41 +0.5 115,362 + H281DRAFT_04400 0.43 +0.4 115,362 + H281DRAFT_04400 0.43 +1.2 115,362 + H281DRAFT_04400 0.43 +1.0 115,362 + H281DRAFT_04400 0.43 +0.4 115,363 - H281DRAFT_04400 0.43 +0.7 115,413 + H281DRAFT_04400 0.46 -0.0 115,450 - H281DRAFT_04400 0.49 -0.2 115,509 - H281DRAFT_04400 0.52 +0.6 115,575 - H281DRAFT_04400 0.56 +0.6 115,668 + H281DRAFT_04400 0.62 +0.9 115,668 + H281DRAFT_04400 0.62 +0.4 115,668 + H281DRAFT_04400 0.62 +0.2 115,669 - H281DRAFT_04400 0.62 +1.0 115,669 - H281DRAFT_04400 0.62 +0.6 116,295 + +0.3 116,295 + -0.4 116,296 - -0.1 116,296 - +0.1 116,373 - +0.2 116,498 + +0.4 116,498 + +0.1 116,499 - -0.1 116,556 + -0.5 116,556 + +0.3 116,556 + +0.9 116,556 + +1.0 116,556 + +0.5 116,556 + +0.7 116,557 - -0.8 116,557 - +0.2 116,557 - +0.4 116,557 - +0.1 116,557 - +0.2 116,557 - -0.6 116,557 - -0.3 116,594 + -0.3 116,690 - H281DRAFT_04401 0.15 +1.1 116,690 - H281DRAFT_04401 0.15 -0.3 116,692 + H281DRAFT_04401 0.16 +0.1 116,692 + H281DRAFT_04401 0.16 +0.4 116,692 + H281DRAFT_04401 0.16 -0.3 116,693 - H281DRAFT_04401 0.16 -0.6 117,107 + H281DRAFT_04401 0.49 -0.5 117,112 + H281DRAFT_04401 0.49 +0.4 117,112 + H281DRAFT_04401 0.49 -0.2 117,112 + H281DRAFT_04401 0.49 -0.5 117,112 + H281DRAFT_04401 0.49 -0.1 117,171 + H281DRAFT_04401 0.54 +0.5 117,171 + H281DRAFT_04401 0.54 -0.3
Or see this region's nucleotide sequence