Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02087 and H281DRAFT_02088 are separated by 94 nucleotides H281DRAFT_02088 and H281DRAFT_02089 overlap by 8 nucleotides H281DRAFT_02089 and H281DRAFT_02090 are separated by 46 nucleotides H281DRAFT_02090 and H281DRAFT_02091 are separated by 12 nucleotides
H281DRAFT_02087: H281DRAFT_02087 - isocitrate dehydrogenase kinase/phosphatase, at 211,469 to 213,316
_02087
H281DRAFT_02088: H281DRAFT_02088 - carbonic anhydrase, at 213,411 to 214,187
_02088
H281DRAFT_02089: H281DRAFT_02089 - acetyl-CoA C-acetyltransferase, at 214,180 to 215,388
_02089
H281DRAFT_02090: H281DRAFT_02090 - Short-chain dehydrogenase, at 215,435 to 216,112
_02090
H281DRAFT_02091: H281DRAFT_02091 - hypothetical protein, at 216,125 to 217,261
_02091
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1 at 213.346 kb on + strand at 213.347 kb on - strand at 213.360 kb on + strand at 213.365 kb on + strand at 213.366 kb on - strand at 213.393 kb on + strand at 213.410 kb on + strand at 213.543 kb on + strand, within H281DRAFT_02088 at 213.543 kb on + strand, within H281DRAFT_02088 at 213.624 kb on - strand, within H281DRAFT_02088 at 213.640 kb on + strand, within H281DRAFT_02088 at 213.650 kb on + strand, within H281DRAFT_02088 at 213.651 kb on - strand, within H281DRAFT_02088 at 213.730 kb on - strand, within H281DRAFT_02088 at 213.979 kb on - strand, within H281DRAFT_02088 at 214.174 kb on + strand at 214.174 kb on + strand at 214.174 kb on + strand at 214.223 kb on + strand at 214.223 kb on + strand at 214.224 kb on - strand at 214.361 kb on + strand, within H281DRAFT_02089 at 214.362 kb on - strand, within H281DRAFT_02089 at 214.362 kb on - strand, within H281DRAFT_02089 at 214.362 kb on - strand, within H281DRAFT_02089 at 214.576 kb on + strand, within H281DRAFT_02089 at 214.577 kb on - strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 215.277 kb on + strand at 215.394 kb on - strand at 215.397 kb on - strand at 215.397 kb on - strand at 215.503 kb on + strand, within H281DRAFT_02090 at 215.504 kb on - strand, within H281DRAFT_02090 at 215.579 kb on + strand, within H281DRAFT_02090 at 215.579 kb on + strand, within H281DRAFT_02090 at 215.675 kb on + strand, within H281DRAFT_02090 at 215.870 kb on + strand, within H281DRAFT_02090 at 215.871 kb on - strand, within H281DRAFT_02090 at 216.087 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 213,346 + +0.2 213,347 - -0.6 213,360 + +0.2 213,365 + +0.2 213,366 - -0.7 213,393 + +0.7 213,410 + -0.2 213,543 + H281DRAFT_02088 0.17 +0.4 213,543 + H281DRAFT_02088 0.17 -0.1 213,624 - H281DRAFT_02088 0.27 +0.2 213,640 + H281DRAFT_02088 0.29 +0.4 213,650 + H281DRAFT_02088 0.31 -0.8 213,651 - H281DRAFT_02088 0.31 +0.3 213,730 - H281DRAFT_02088 0.41 -0.5 213,979 - H281DRAFT_02088 0.73 -0.4 214,174 + +0.8 214,174 + +0.4 214,174 + -0.1 214,223 + -0.4 214,223 + -0.0 214,224 - +0.0 214,361 + H281DRAFT_02089 0.15 +0.0 214,362 - H281DRAFT_02089 0.15 -0.6 214,362 - H281DRAFT_02089 0.15 +0.4 214,362 - H281DRAFT_02089 0.15 +0.3 214,576 + H281DRAFT_02089 0.33 +0.2 214,577 - H281DRAFT_02089 0.33 -0.1 214,635 + H281DRAFT_02089 0.38 -0.2 214,635 + H281DRAFT_02089 0.38 +0.0 214,635 + H281DRAFT_02089 0.38 +0.0 214,635 + H281DRAFT_02089 0.38 +0.2 214,635 + H281DRAFT_02089 0.38 -0.7 214,635 + H281DRAFT_02089 0.38 -0.3 214,635 + H281DRAFT_02089 0.38 -2.5 214,636 - H281DRAFT_02089 0.38 -0.0 214,636 - H281DRAFT_02089 0.38 +0.2 214,636 - H281DRAFT_02089 0.38 +0.0 214,636 - H281DRAFT_02089 0.38 -0.5 214,636 - H281DRAFT_02089 0.38 -0.5 214,636 - H281DRAFT_02089 0.38 -0.2 215,277 + -0.4 215,394 - -0.1 215,397 - +0.4 215,397 - +0.0 215,503 + H281DRAFT_02090 0.10 -0.1 215,504 - H281DRAFT_02090 0.10 -0.3 215,579 + H281DRAFT_02090 0.21 +0.9 215,579 + H281DRAFT_02090 0.21 +0.4 215,675 + H281DRAFT_02090 0.35 -0.5 215,870 + H281DRAFT_02090 0.64 -0.2 215,871 - H281DRAFT_02090 0.64 -1.2 216,087 - +0.1
Or see this region's nucleotide sequence