Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01343 and H281DRAFT_01344 are separated by 72 nucleotides H281DRAFT_01344 and H281DRAFT_01345 are separated by 28 nucleotides H281DRAFT_01345 and H281DRAFT_01346 are separated by 97 nucleotides
H281DRAFT_01343: H281DRAFT_01343 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 183,959 to 185,992
_01343
H281DRAFT_01344: H281DRAFT_01344 - methylglutaconyl-CoA hydratase, at 186,065 to 186,850
_01344
H281DRAFT_01345: H281DRAFT_01345 - 3-methylcrotonyl-CoA carboxylase beta subunit, at 186,879 to 188,486
_01345
H281DRAFT_01346: H281DRAFT_01346 - isovaleryl-CoA dehydrogenase, at 188,584 to 189,765
_01346
Position (kb) 186
187
188
189 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 186.843 kb on + strand at 186.905 kb on + strand at 186.906 kb on - strand at 187.010 kb on - strand at 187.012 kb on + strand at 187.033 kb on + strand at 187.034 kb on - strand at 187.222 kb on + strand, within H281DRAFT_01345 at 187.374 kb on + strand, within H281DRAFT_01345 at 187.487 kb on + strand, within H281DRAFT_01345 at 187.487 kb on + strand, within H281DRAFT_01345 at 187.619 kb on - strand, within H281DRAFT_01345 at 187.637 kb on - strand, within H281DRAFT_01345 at 187.772 kb on + strand, within H281DRAFT_01345 at 187.773 kb on - strand, within H281DRAFT_01345 at 187.773 kb on - strand, within H281DRAFT_01345 at 187.773 kb on - strand, within H281DRAFT_01345 at 187.773 kb on - strand, within H281DRAFT_01345 at 187.906 kb on + strand, within H281DRAFT_01345 at 187.984 kb on + strand, within H281DRAFT_01345 at 188.065 kb on + strand, within H281DRAFT_01345 at 188.065 kb on + strand, within H281DRAFT_01345 at 188.066 kb on - strand, within H281DRAFT_01345 at 188.137 kb on + strand, within H281DRAFT_01345 at 188.138 kb on - strand at 188.242 kb on + strand, within H281DRAFT_01345 at 188.242 kb on + strand, within H281DRAFT_01345 at 188.243 kb on - strand, within H281DRAFT_01345 at 188.585 kb on + strand at 188.658 kb on - strand at 188.769 kb on - strand, within H281DRAFT_01346 at 188.849 kb on + strand, within H281DRAFT_01346 at 189.204 kb on + strand, within H281DRAFT_01346 at 189.364 kb on - strand, within H281DRAFT_01346 at 189.419 kb on + strand, within H281DRAFT_01346 at 189.419 kb on + strand, within H281DRAFT_01346 at 189.470 kb on - strand, within H281DRAFT_01346
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 186,843 + -3.6 186,905 + -4.6 186,906 - -2.7 187,010 - -3.5 187,012 + -4.6 187,033 + -4.9 187,034 - -3.2 187,222 + H281DRAFT_01345 0.21 -3.6 187,374 + H281DRAFT_01345 0.31 -2.1 187,487 + H281DRAFT_01345 0.38 -4.6 187,487 + H281DRAFT_01345 0.38 -1.6 187,619 - H281DRAFT_01345 0.46 -2.6 187,637 - H281DRAFT_01345 0.47 -2.4 187,772 + H281DRAFT_01345 0.56 -5.3 187,773 - H281DRAFT_01345 0.56 -2.9 187,773 - H281DRAFT_01345 0.56 -4.5 187,773 - H281DRAFT_01345 0.56 -4.8 187,773 - H281DRAFT_01345 0.56 -3.7 187,906 + H281DRAFT_01345 0.64 -3.8 187,984 + H281DRAFT_01345 0.69 -3.8 188,065 + H281DRAFT_01345 0.74 -2.7 188,065 + H281DRAFT_01345 0.74 -3.3 188,066 - H281DRAFT_01345 0.74 -4.4 188,137 + H281DRAFT_01345 0.78 -4.0 188,138 - -0.6 188,242 + H281DRAFT_01345 0.85 -2.1 188,242 + H281DRAFT_01345 0.85 -3.6 188,243 - H281DRAFT_01345 0.85 -3.8 188,585 + -4.7 188,658 - -5.4 188,769 - H281DRAFT_01346 0.16 -5.8 188,849 + H281DRAFT_01346 0.22 -4.7 189,204 + H281DRAFT_01346 0.52 -4.0 189,364 - H281DRAFT_01346 0.66 -1.4 189,419 + H281DRAFT_01346 0.71 -1.1 189,419 + H281DRAFT_01346 0.71 -1.9 189,470 - H281DRAFT_01346 0.75 -3.4
Or see this region's nucleotide sequence