Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00366 and H281DRAFT_00367 are separated by 78 nucleotides H281DRAFT_00367 and H281DRAFT_00368 are separated by 179 nucleotides H281DRAFT_00368 and H281DRAFT_00369 are separated by 8 nucleotides
H281DRAFT_00366: H281DRAFT_00366 - phospholipase C, at 400,875 to 402,986
_00366
H281DRAFT_00367: H281DRAFT_00367 - hypothetical protein, at 403,065 to 403,388
_00367
H281DRAFT_00368: H281DRAFT_00368 - Pimeloyl-ACP methyl ester carboxylesterase, at 403,568 to 404,422
_00368
H281DRAFT_00369: H281DRAFT_00369 - benzoate-CoA ligase (EC 6.2.1.25), at 404,431 to 406,035
_00369
Position (kb) 403
404
405 Strain fitness (log2 ratio) -1
0
1 at 402.618 kb on - strand, within H281DRAFT_00366 at 403.827 kb on + strand, within H281DRAFT_00368 at 403.925 kb on + strand, within H281DRAFT_00368 at 403.940 kb on + strand, within H281DRAFT_00368 at 404.424 kb on + strand at 404.712 kb on - strand, within H281DRAFT_00369 at 404.712 kb on - strand, within H281DRAFT_00369 at 404.753 kb on + strand, within H281DRAFT_00369 at 404.760 kb on - strand, within H281DRAFT_00369 at 404.849 kb on + strand, within H281DRAFT_00369 at 405.006 kb on + strand, within H281DRAFT_00369 at 405.006 kb on + strand, within H281DRAFT_00369 at 405.063 kb on + strand, within H281DRAFT_00369 at 405.312 kb on - strand, within H281DRAFT_00369
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 402,618 - H281DRAFT_00366 0.83 +0.6 403,827 + H281DRAFT_00368 0.30 +0.5 403,925 + H281DRAFT_00368 0.42 +0.3 403,940 + H281DRAFT_00368 0.44 +0.4 404,424 + +0.3 404,712 - H281DRAFT_00369 0.18 -0.4 404,712 - H281DRAFT_00369 0.18 -0.4 404,753 + H281DRAFT_00369 0.20 -0.2 404,760 - H281DRAFT_00369 0.20 -0.0 404,849 + H281DRAFT_00369 0.26 +0.7 405,006 + H281DRAFT_00369 0.36 -0.1 405,006 + H281DRAFT_00369 0.36 +0.8 405,063 + H281DRAFT_00369 0.39 +0.4 405,312 - H281DRAFT_00369 0.55 -0.2
Or see this region's nucleotide sequence