Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00139 and H281DRAFT_00140 are separated by 46 nucleotides H281DRAFT_00140 and H281DRAFT_00141 overlap by 16 nucleotides H281DRAFT_00141 and H281DRAFT_00142 are separated by 45 nucleotides
H281DRAFT_00139: H281DRAFT_00139 - nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, at 144,892 to 145,953
_00139
H281DRAFT_00140: H281DRAFT_00140 - cobalamin-5'-phosphate synthase, at 146,000 to 146,785
_00140
H281DRAFT_00141: H281DRAFT_00141 - alpha-ribazole phosphatase, at 146,770 to 147,360
_00141
H281DRAFT_00142: H281DRAFT_00142 - iron complex transport system substrate-binding protein, at 147,406 to 148,326
_00142
Position (kb) 146
147
148 Strain fitness (log2 ratio) -1
0
1 at 145.801 kb on + strand, within H281DRAFT_00139 at 145.824 kb on - strand, within H281DRAFT_00139 at 145.997 kb on + strand at 145.998 kb on - strand at 145.998 kb on - strand at 145.998 kb on - strand at 145.999 kb on + strand at 146.000 kb on - strand at 146.000 kb on - strand at 146.059 kb on - strand at 146.081 kb on + strand, within H281DRAFT_00140 at 146.082 kb on - strand, within H281DRAFT_00140 at 146.094 kb on - strand, within H281DRAFT_00140 at 146.162 kb on + strand, within H281DRAFT_00140 at 146.162 kb on + strand, within H281DRAFT_00140 at 146.469 kb on - strand, within H281DRAFT_00140 at 146.486 kb on + strand, within H281DRAFT_00140 at 146.487 kb on - strand, within H281DRAFT_00140 at 146.548 kb on - strand, within H281DRAFT_00140 at 146.678 kb on + strand, within H281DRAFT_00140 at 146.753 kb on + strand at 146.754 kb on - strand at 146.754 kb on - strand at 146.760 kb on + strand at 146.886 kb on - strand, within H281DRAFT_00141 at 146.886 kb on - strand, within H281DRAFT_00141 at 146.886 kb on - strand, within H281DRAFT_00141 at 147.199 kb on + strand, within H281DRAFT_00141 at 147.200 kb on - strand, within H281DRAFT_00141 at 147.200 kb on - strand, within H281DRAFT_00141 at 147.389 kb on - strand at 147.531 kb on + strand, within H281DRAFT_00142 at 147.716 kb on - strand, within H281DRAFT_00142 at 147.738 kb on + strand, within H281DRAFT_00142 at 147.738 kb on + strand, within H281DRAFT_00142 at 147.738 kb on + strand at 147.845 kb on + strand, within H281DRAFT_00142 at 147.955 kb on + strand, within H281DRAFT_00142 at 147.955 kb on + strand, within H281DRAFT_00142 at 147.956 kb on - strand, within H281DRAFT_00142 at 148.017 kb on + strand, within H281DRAFT_00142 at 148.018 kb on - strand, within H281DRAFT_00142 at 148.126 kb on + strand, within H281DRAFT_00142 at 148.190 kb on - strand, within H281DRAFT_00142 at 148.346 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 145,801 + H281DRAFT_00139 0.86 -0.4 145,824 - H281DRAFT_00139 0.88 +0.4 145,997 + -0.3 145,998 - -0.6 145,998 - +0.2 145,998 - -0.1 145,999 + +0.3 146,000 - -0.2 146,000 - -0.1 146,059 - +0.3 146,081 + H281DRAFT_00140 0.10 +0.3 146,082 - H281DRAFT_00140 0.10 -0.4 146,094 - H281DRAFT_00140 0.12 -0.8 146,162 + H281DRAFT_00140 0.21 +0.2 146,162 + H281DRAFT_00140 0.21 +0.2 146,469 - H281DRAFT_00140 0.60 +0.1 146,486 + H281DRAFT_00140 0.62 +0.0 146,487 - H281DRAFT_00140 0.62 +0.8 146,548 - H281DRAFT_00140 0.70 -0.9 146,678 + H281DRAFT_00140 0.86 +0.3 146,753 + +0.3 146,754 - +0.5 146,754 - -1.0 146,760 + +1.1 146,886 - H281DRAFT_00141 0.20 +0.5 146,886 - H281DRAFT_00141 0.20 +0.2 146,886 - H281DRAFT_00141 0.20 +0.5 147,199 + H281DRAFT_00141 0.73 +0.6 147,200 - H281DRAFT_00141 0.73 +0.1 147,200 - H281DRAFT_00141 0.73 +1.2 147,389 - +0.6 147,531 + H281DRAFT_00142 0.14 +0.4 147,716 - H281DRAFT_00142 0.34 -0.0 147,738 + H281DRAFT_00142 0.36 -0.0 147,738 + H281DRAFT_00142 0.36 +0.8 147,738 + +0.5 147,845 + H281DRAFT_00142 0.48 +0.4 147,955 + H281DRAFT_00142 0.60 -0.2 147,955 + H281DRAFT_00142 0.60 -0.3 147,956 - H281DRAFT_00142 0.60 -0.5 148,017 + H281DRAFT_00142 0.66 +0.7 148,018 - H281DRAFT_00142 0.66 -0.2 148,126 + H281DRAFT_00142 0.78 -0.1 148,190 - H281DRAFT_00142 0.85 +0.0 148,346 + -0.2
Or see this region's nucleotide sequence