Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3647 and Dshi_3648 are separated by 4 nucleotides Dshi_3648 and Dshi_3649 overlap by 4 nucleotides Dshi_3649 and Dshi_3650 overlap by 4 nucleotides Dshi_3650 and Dshi_3651 are separated by 12 nucleotides
Dshi_3647: Dshi_3647 - conjugation TrbI family protein (RefSeq), at 46,205 to 47,584
_3647
Dshi_3648: Dshi_3648 - type II secretion system protein E (RefSeq), at 47,589 to 48,575
_3648
Dshi_3649: Dshi_3649 - hypothetical protein (RefSeq), at 48,572 to 48,766
_3649
Dshi_3650: Dshi_3650 - hypothetical protein (RefSeq), at 48,763 to 49,173
_3650
Dshi_3651: Dshi_3651 - TrbL/VirB6 plasmid conjugal transfer protein (RefSeq), at 49,186 to 50,262
_3651
Position (kb)
47
48
49 Strain fitness (log2 ratio)
-1
0
1
2 at 46.701 kb on + strand, within Dshi_3647 at 46.897 kb on + strand, within Dshi_3647 at 46.954 kb on - strand, within Dshi_3647 at 47.149 kb on + strand, within Dshi_3647 at 47.181 kb on + strand, within Dshi_3647 at 47.181 kb on + strand, within Dshi_3647 at 47.230 kb on - strand, within Dshi_3647 at 47.287 kb on - strand, within Dshi_3647 at 47.293 kb on + strand, within Dshi_3647 at 47.381 kb on - strand, within Dshi_3647 at 47.447 kb on + strand at 47.473 kb on - strand at 47.504 kb on - strand at 47.719 kb on + strand, within Dshi_3648 at 47.755 kb on - strand, within Dshi_3648 at 47.837 kb on - strand, within Dshi_3648 at 47.838 kb on - strand, within Dshi_3648 at 47.838 kb on - strand, within Dshi_3648 at 47.839 kb on - strand, within Dshi_3648 at 47.839 kb on - strand, within Dshi_3648 at 47.859 kb on - strand, within Dshi_3648 at 48.008 kb on + strand, within Dshi_3648 at 48.130 kb on + strand, within Dshi_3648 at 48.138 kb on - strand, within Dshi_3648 at 48.168 kb on - strand, within Dshi_3648 at 48.178 kb on + strand, within Dshi_3648 at 48.178 kb on + strand, within Dshi_3648 at 48.186 kb on - strand, within Dshi_3648 at 48.194 kb on + strand, within Dshi_3648 at 48.202 kb on + strand, within Dshi_3648 at 48.202 kb on - strand, within Dshi_3648 at 48.210 kb on - strand, within Dshi_3648 at 48.267 kb on - strand, within Dshi_3648 at 48.317 kb on + strand, within Dshi_3648 at 48.318 kb on + strand, within Dshi_3648 at 48.351 kb on - strand, within Dshi_3648 at 48.367 kb on - strand, within Dshi_3648 at 48.385 kb on + strand, within Dshi_3648 at 48.387 kb on + strand, within Dshi_3648 at 48.407 kb on - strand, within Dshi_3648 at 48.456 kb on - strand, within Dshi_3648 at 48.555 kb on - strand at 48.605 kb on + strand, within Dshi_3649 at 48.638 kb on - strand, within Dshi_3649 at 48.643 kb on - strand, within Dshi_3649 at 48.652 kb on - strand, within Dshi_3649 at 48.669 kb on + strand, within Dshi_3649 at 48.669 kb on + strand, within Dshi_3649 at 48.669 kb on + strand, within Dshi_3649 at 48.669 kb on + strand, within Dshi_3649 at 48.669 kb on + strand, within Dshi_3649 at 48.669 kb on + strand, within Dshi_3649 at 48.669 kb on + strand, within Dshi_3649 at 48.685 kb on + strand, within Dshi_3649 at 48.720 kb on + strand, within Dshi_3649 at 48.748 kb on - strand at 48.768 kb on - strand at 48.769 kb on - strand at 48.780 kb on - strand at 48.939 kb on + strand, within Dshi_3650 at 48.954 kb on + strand, within Dshi_3650 at 49.020 kb on - strand, within Dshi_3650 at 49.039 kb on - strand, within Dshi_3650 at 49.042 kb on - strand, within Dshi_3650 at 49.103 kb on + strand, within Dshi_3650 at 49.181 kb on - strand at 49.221 kb on + strand at 49.222 kb on - strand at 49.223 kb on - strand at 49.233 kb on - strand at 49.235 kb on - strand at 49.248 kb on - strand at 49.288 kb on - strand at 49.295 kb on + strand, within Dshi_3651 at 49.425 kb on + strand, within Dshi_3651 at 49.452 kb on + strand, within Dshi_3651 at 49.486 kb on + strand, within Dshi_3651 at 49.488 kb on - strand, within Dshi_3651 at 49.513 kb on - strand, within Dshi_3651 at 49.513 kb on - strand, within Dshi_3651
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 46,701 + Dshi_3647 0.36 -1.3 46,897 + Dshi_3647 0.50 -0.2 46,954 - Dshi_3647 0.54 +0.5 47,149 + Dshi_3647 0.68 +0.1 47,181 + Dshi_3647 0.71 +0.1 47,181 + Dshi_3647 0.71 +0.3 47,230 - Dshi_3647 0.74 -0.2 47,287 - Dshi_3647 0.78 +0.1 47,293 + Dshi_3647 0.79 +0.2 47,381 - Dshi_3647 0.85 +0.3 47,447 + -0.5 47,473 - -0.3 47,504 - +0.3 47,719 + Dshi_3648 0.13 +0.6 47,755 - Dshi_3648 0.17 -0.3 47,837 - Dshi_3648 0.25 -0.7 47,838 - Dshi_3648 0.25 +0.2 47,838 - Dshi_3648 0.25 +0.2 47,839 - Dshi_3648 0.25 -0.0 47,839 - Dshi_3648 0.25 -0.3 47,859 - Dshi_3648 0.27 +2.0 48,008 + Dshi_3648 0.42 -0.1 48,130 + Dshi_3648 0.55 -0.3 48,138 - Dshi_3648 0.56 -0.0 48,168 - Dshi_3648 0.59 -0.3 48,178 + Dshi_3648 0.60 -1.0 48,178 + Dshi_3648 0.60 -0.7 48,186 - Dshi_3648 0.60 +0.9 48,194 + Dshi_3648 0.61 +0.3 48,202 + Dshi_3648 0.62 +0.0 48,202 - Dshi_3648 0.62 +0.3 48,210 - Dshi_3648 0.63 -0.0 48,267 - Dshi_3648 0.69 +0.1 48,317 + Dshi_3648 0.74 -1.0 48,318 + Dshi_3648 0.74 -0.2 48,351 - Dshi_3648 0.77 +0.0 48,367 - Dshi_3648 0.79 -0.7 48,385 + Dshi_3648 0.81 -0.2 48,387 + Dshi_3648 0.81 +0.0 48,407 - Dshi_3648 0.83 +0.1 48,456 - Dshi_3648 0.88 +0.9 48,555 - +0.1 48,605 + Dshi_3649 0.17 +1.1 48,638 - Dshi_3649 0.34 -1.0 48,643 - Dshi_3649 0.36 -1.4 48,652 - Dshi_3649 0.41 +0.2 48,669 + Dshi_3649 0.50 -0.9 48,669 + Dshi_3649 0.50 +1.4 48,669 + Dshi_3649 0.50 -0.5 48,669 + Dshi_3649 0.50 -0.6 48,669 + Dshi_3649 0.50 -0.0 48,669 + Dshi_3649 0.50 +0.5 48,669 + Dshi_3649 0.50 -0.5 48,685 + Dshi_3649 0.58 -0.4 48,720 + Dshi_3649 0.76 -1.1 48,748 - +1.2 48,768 - -0.0 48,769 - -0.5 48,780 - -0.4 48,939 + Dshi_3650 0.43 +0.2 48,954 + Dshi_3650 0.46 -0.3 49,020 - Dshi_3650 0.63 -0.3 49,039 - Dshi_3650 0.67 +1.0 49,042 - Dshi_3650 0.68 -0.3 49,103 + Dshi_3650 0.83 -0.6 49,181 - +0.3 49,221 + -0.7 49,222 - -0.3 49,223 - -0.8 49,233 - -0.1 49,235 - +0.4 49,248 - -0.8 49,288 - +0.2 49,295 + Dshi_3651 0.10 -0.8 49,425 + Dshi_3651 0.22 +1.0 49,452 + Dshi_3651 0.25 -0.2 49,486 + Dshi_3651 0.28 +0.2 49,488 - Dshi_3651 0.28 -0.1 49,513 - Dshi_3651 0.30 -0.0 49,513 - Dshi_3651 0.30 +0.4
Or see this region's nucleotide sequence