Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1927 and Dshi_1928 are separated by 57 nucleotides Dshi_1928 and xerC are separated by 3 nucleotides xerC and Dshi_1930 are separated by 19 nucleotides Dshi_1930 and Dshi_1931 are separated by 62 nucleotides
Dshi_1927: Dshi_1927 - putative transaldolase (RefSeq), at 2,014,831 to 2,015,484
_1927
Dshi_1928: Dshi_1928 - protein of unknown function DUF484 (RefSeq), at 2,015,542 to 2,016,249
_1928
Dshi_5002: xerC - hypothetical protein (NCBI), at 2,016,253 to 2,017,179
xerC
Dshi_1930: Dshi_1930 - protein of unknown function DUF6 transmembrane (RefSeq), at 2,017,199 to 2,018,038
_1930
Dshi_1931: Dshi_1931 - hypothetical protein (RefSeq), at 2,018,101 to 2,018,352
_1931
Position (kb)
2016
2017
2018 Strain fitness (log2 ratio)
-2
-1
0
1 at 2015.257 kb on - strand, within Dshi_1927 at 2015.308 kb on - strand, within Dshi_1927 at 2015.538 kb on + strand at 2015.546 kb on - strand at 2015.599 kb on + strand at 2015.695 kb on - strand, within Dshi_1928 at 2016.345 kb on + strand at 2017.121 kb on - strand at 2017.121 kb on - strand at 2017.160 kb on - strand at 2017.173 kb on + strand at 2017.181 kb on - strand at 2017.221 kb on - strand at 2017.573 kb on - strand, within Dshi_1930 at 2017.619 kb on + strand, within Dshi_1930 at 2017.838 kb on - strand, within Dshi_1930 at 2018.082 kb on - strand at 2018.105 kb on - strand at 2018.130 kb on + strand, within Dshi_1931
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 2,015,257 - Dshi_1927 0.65 -2.2 2,015,308 - Dshi_1927 0.73 -0.4 2,015,538 + -0.4 2,015,546 - +0.3 2,015,599 + -1.9 2,015,695 - Dshi_1928 0.22 -1.1 2,016,345 + +0.9 2,017,121 - +0.5 2,017,121 - -0.7 2,017,160 - -0.2 2,017,173 + -0.7 2,017,181 - -0.5 2,017,221 - -0.2 2,017,573 - Dshi_1930 0.45 +0.3 2,017,619 + Dshi_1930 0.50 -0.1 2,017,838 - Dshi_1930 0.76 -0.3 2,018,082 - -0.5 2,018,105 - +0.9 2,018,130 + Dshi_1931 0.12 +0.1
Or see this region's nucleotide sequence