Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0123 and Dshi_0124 are separated by 145 nucleotides Dshi_0124 and Dshi_0125 are separated by 38 nucleotides Dshi_0125 and Dshi_0126 are separated by 103 nucleotides Dshi_0126 and Dshi_0127 overlap by 4 nucleotides
Dshi_0123: Dshi_0123 - transcriptional regulator, LysR family (RefSeq), at 121,186 to 122,094
_0123
Dshi_0124: Dshi_0124 - intracellular protease, PfpI family (RefSeq), at 122,240 to 122,824
_0124
Dshi_0125: Dshi_0125 - NADH:flavin oxidoreductase/NADH oxidase (RefSeq), at 122,863 to 124,881
_0125
Dshi_0126: Dshi_0126 - hypothetical protein (RefSeq), at 124,985 to 125,401
_0126
Dshi_0127: Dshi_0127 - hypothetical protein (RefSeq), at 125,398 to 126,168
_0127
Position (kb)
122
123
124
125 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 121.915 kb on - strand, within Dshi_0123 at 121.974 kb on + strand, within Dshi_0123 at 122.049 kb on + strand at 122.058 kb on + strand at 122.102 kb on + strand at 122.103 kb on + strand at 122.103 kb on + strand at 122.109 kb on - strand at 122.112 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.130 kb on - strand at 122.132 kb on - strand at 122.132 kb on - strand at 122.132 kb on - strand at 122.146 kb on + strand at 122.156 kb on + strand at 122.243 kb on + strand at 122.248 kb on + strand at 122.335 kb on + strand, within Dshi_0124 at 122.343 kb on - strand, within Dshi_0124 at 122.369 kb on - strand, within Dshi_0124 at 122.392 kb on - strand, within Dshi_0124 at 122.392 kb on - strand, within Dshi_0124 at 122.484 kb on + strand, within Dshi_0124 at 122.510 kb on + strand, within Dshi_0124 at 122.562 kb on + strand, within Dshi_0124 at 122.572 kb on + strand, within Dshi_0124 at 122.580 kb on - strand, within Dshi_0124 at 122.635 kb on + strand, within Dshi_0124 at 122.721 kb on + strand, within Dshi_0124 at 122.745 kb on - strand, within Dshi_0124 at 122.819 kb on - strand at 122.829 kb on - strand at 122.856 kb on + strand at 122.888 kb on + strand at 122.936 kb on - strand at 123.011 kb on - strand at 123.013 kb on + strand at 123.015 kb on + strand at 123.021 kb on - strand at 123.072 kb on + strand, within Dshi_0125 at 123.232 kb on - strand, within Dshi_0125 at 123.330 kb on - strand, within Dshi_0125 at 123.423 kb on - strand, within Dshi_0125 at 123.592 kb on + strand, within Dshi_0125 at 123.600 kb on - strand, within Dshi_0125 at 123.646 kb on - strand, within Dshi_0125 at 123.782 kb on - strand, within Dshi_0125 at 123.819 kb on + strand, within Dshi_0125 at 123.832 kb on - strand, within Dshi_0125 at 123.865 kb on + strand, within Dshi_0125 at 123.867 kb on - strand, within Dshi_0125 at 123.906 kb on - strand, within Dshi_0125 at 123.925 kb on + strand, within Dshi_0125 at 124.106 kb on + strand, within Dshi_0125 at 124.200 kb on + strand, within Dshi_0125 at 124.314 kb on - strand, within Dshi_0125 at 124.409 kb on - strand, within Dshi_0125 at 124.449 kb on + strand, within Dshi_0125 at 124.556 kb on + strand, within Dshi_0125 at 124.645 kb on - strand, within Dshi_0125 at 124.660 kb on - strand, within Dshi_0125 at 124.756 kb on - strand at 124.920 kb on + strand at 124.928 kb on - strand at 124.936 kb on - strand at 124.952 kb on - strand at 125.062 kb on - strand, within Dshi_0126 at 125.095 kb on + strand, within Dshi_0126 at 125.125 kb on - strand, within Dshi_0126 at 125.198 kb on + strand, within Dshi_0126 at 125.283 kb on - strand, within Dshi_0126 at 125.761 kb on - strand, within Dshi_0127
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 121,915 - Dshi_0123 0.80 -1.1 121,974 + Dshi_0123 0.87 -0.1 122,049 + -0.1 122,058 + +0.4 122,102 + -0.0 122,103 + -0.6 122,103 + +0.5 122,109 - -0.6 122,112 + -0.4 122,124 + -0.3 122,124 + +2.5 122,124 + -0.0 122,130 - +0.3 122,132 - -0.3 122,132 - +0.6 122,132 - +0.8 122,146 + -1.1 122,156 + -0.1 122,243 + +0.9 122,248 + +0.4 122,335 + Dshi_0124 0.16 +0.6 122,343 - Dshi_0124 0.18 -0.1 122,369 - Dshi_0124 0.22 -1.1 122,392 - Dshi_0124 0.26 -0.2 122,392 - Dshi_0124 0.26 -1.0 122,484 + Dshi_0124 0.42 +0.6 122,510 + Dshi_0124 0.46 -0.0 122,562 + Dshi_0124 0.55 -0.5 122,572 + Dshi_0124 0.57 -1.4 122,580 - Dshi_0124 0.58 +0.3 122,635 + Dshi_0124 0.68 -0.7 122,721 + Dshi_0124 0.82 +0.3 122,745 - Dshi_0124 0.86 -0.0 122,819 - -0.8 122,829 - -0.6 122,856 + -1.4 122,888 + +0.1 122,936 - -0.1 123,011 - +0.4 123,013 + +1.9 123,015 + -1.4 123,021 - +0.1 123,072 + Dshi_0125 0.10 -1.1 123,232 - Dshi_0125 0.18 -0.2 123,330 - Dshi_0125 0.23 -1.8 123,423 - Dshi_0125 0.28 +0.3 123,592 + Dshi_0125 0.36 -1.0 123,600 - Dshi_0125 0.37 -0.2 123,646 - Dshi_0125 0.39 -0.4 123,782 - Dshi_0125 0.46 -0.4 123,819 + Dshi_0125 0.47 +0.0 123,832 - Dshi_0125 0.48 -0.3 123,865 + Dshi_0125 0.50 -0.6 123,867 - Dshi_0125 0.50 +0.1 123,906 - Dshi_0125 0.52 -1.1 123,925 + Dshi_0125 0.53 +0.5 124,106 + Dshi_0125 0.62 -0.5 124,200 + Dshi_0125 0.66 +0.1 124,314 - Dshi_0125 0.72 +0.2 124,409 - Dshi_0125 0.77 -0.1 124,449 + Dshi_0125 0.79 +0.4 124,556 + Dshi_0125 0.84 -1.0 124,645 - Dshi_0125 0.88 -2.1 124,660 - Dshi_0125 0.89 -0.2 124,756 - -0.4 124,920 + +0.0 124,928 - -2.1 124,936 - -0.8 124,952 - -0.0 125,062 - Dshi_0126 0.18 -0.3 125,095 + Dshi_0126 0.26 +0.2 125,125 - Dshi_0126 0.34 +0.4 125,198 + Dshi_0126 0.51 -0.2 125,283 - Dshi_0126 0.71 +1.1 125,761 - Dshi_0127 0.47 +1.8
Or see this region's nucleotide sequence