Experiment: MoYLS4 with FCCP 2uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiB-2 and clpB are separated by 302 nucleotides clpB and DVU1875 are separated by 24 nucleotides DVU1875 and dnaJ are separated by 32 nucleotides
DVU1873: ppiB-2 - peptidyl-prolyl cis-trans isomerase B (TIGR), at 1,940,074 to 1,940,568
ppiB-2
DVU1874: clpB - ATP-dependent Clp protease, ATP-binding subunit ClpB (TIGR), at 1,940,871 to 1,943,468
clpB
DVU1875: DVU1875 - dafA protein (TIGR), at 1,943,493 to 1,943,810
DVU1875
DVU1876: dnaJ - dnaJ protein, putative (TIGR), at 1,943,843 to 1,944,793
dnaJ
Position (kb)
1940
1941
1942
1943
1944 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1939.909 kb on - strand at 1940.058 kb on + strand at 1940.156 kb on + strand, within ppiB-2 at 1940.156 kb on + strand, within ppiB-2 at 1940.156 kb on + strand, within ppiB-2 at 1940.164 kb on - strand, within ppiB-2 at 1940.182 kb on - strand, within ppiB-2 at 1940.190 kb on - strand, within ppiB-2 at 1940.212 kb on + strand, within ppiB-2 at 1940.587 kb on - strand at 1940.607 kb on + strand at 1940.632 kb on - strand at 1940.729 kb on - strand at 1940.894 kb on - strand at 1940.904 kb on - strand at 1940.914 kb on - strand at 1941.116 kb on - strand at 1941.154 kb on + strand, within clpB at 1941.154 kb on + strand, within clpB at 1941.168 kb on + strand, within clpB at 1941.227 kb on - strand, within clpB at 1941.275 kb on - strand, within clpB at 1941.388 kb on + strand, within clpB at 1941.396 kb on - strand, within clpB at 1941.398 kb on + strand, within clpB at 1941.398 kb on + strand, within clpB at 1941.406 kb on - strand, within clpB at 1941.492 kb on - strand, within clpB at 1941.519 kb on - strand, within clpB at 1941.673 kb on + strand, within clpB at 1941.739 kb on - strand, within clpB at 1941.828 kb on - strand, within clpB at 1941.986 kb on + strand, within clpB at 1942.033 kb on - strand, within clpB at 1942.064 kb on - strand, within clpB at 1942.092 kb on - strand, within clpB at 1942.151 kb on + strand, within clpB at 1942.183 kb on + strand, within clpB at 1942.191 kb on - strand, within clpB at 1942.191 kb on - strand, within clpB at 1942.198 kb on + strand, within clpB at 1942.198 kb on + strand, within clpB at 1942.246 kb on + strand, within clpB at 1942.246 kb on + strand, within clpB at 1942.369 kb on + strand, within clpB at 1942.463 kb on + strand, within clpB at 1942.554 kb on + strand, within clpB at 1942.701 kb on + strand, within clpB at 1942.762 kb on - strand, within clpB at 1942.783 kb on + strand, within clpB at 1942.801 kb on + strand, within clpB at 1942.802 kb on - strand, within clpB at 1942.857 kb on - strand, within clpB at 1942.857 kb on - strand, within clpB at 1942.965 kb on + strand, within clpB at 1943.039 kb on - strand, within clpB at 1943.059 kb on - strand, within clpB at 1943.102 kb on - strand, within clpB at 1943.187 kb on - strand, within clpB at 1943.431 kb on - strand at 1943.600 kb on - strand, within DVU1875 at 1943.643 kb on - strand, within DVU1875 at 1943.796 kb on + strand at 1943.809 kb on + strand at 1943.834 kb on + strand at 1943.879 kb on + strand at 1943.887 kb on - strand at 1943.888 kb on + strand at 1943.974 kb on + strand, within dnaJ at 1943.974 kb on + strand, within dnaJ at 1943.974 kb on + strand, within dnaJ at 1943.974 kb on + strand, within dnaJ at 1943.982 kb on - strand, within dnaJ at 1943.982 kb on - strand, within dnaJ at 1944.060 kb on + strand, within dnaJ at 1944.060 kb on + strand, within dnaJ at 1944.067 kb on + strand, within dnaJ at 1944.122 kb on + strand, within dnaJ at 1944.172 kb on + strand, within dnaJ at 1944.421 kb on - strand, within dnaJ
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with FCCP 2uM remove 1,939,909 - -1.9 1,940,058 + +0.1 1,940,156 + ppiB-2 DVU1873 0.17 +0.4 1,940,156 + ppiB-2 DVU1873 0.17 -0.5 1,940,156 + ppiB-2 DVU1873 0.17 -0.5 1,940,164 - ppiB-2 DVU1873 0.18 +1.2 1,940,182 - ppiB-2 DVU1873 0.22 -0.7 1,940,190 - ppiB-2 DVU1873 0.23 -1.3 1,940,212 + ppiB-2 DVU1873 0.28 -1.4 1,940,587 - -0.6 1,940,607 + -0.6 1,940,632 - -0.3 1,940,729 - -0.3 1,940,894 - +0.7 1,940,904 - +2.0 1,940,914 - -1.6 1,941,116 - -0.9 1,941,154 + clpB DVU1874 0.11 +1.0 1,941,154 + clpB DVU1874 0.11 +1.0 1,941,168 + clpB DVU1874 0.11 -2.4 1,941,227 - clpB DVU1874 0.14 +0.0 1,941,275 - clpB DVU1874 0.16 -1.1 1,941,388 + clpB DVU1874 0.20 +1.0 1,941,396 - clpB DVU1874 0.20 +1.4 1,941,398 + clpB DVU1874 0.20 +3.0 1,941,398 + clpB DVU1874 0.20 +0.8 1,941,406 - clpB DVU1874 0.21 -1.6 1,941,492 - clpB DVU1874 0.24 -1.6 1,941,519 - clpB DVU1874 0.25 -0.2 1,941,673 + clpB DVU1874 0.31 -0.9 1,941,739 - clpB DVU1874 0.33 -0.3 1,941,828 - clpB DVU1874 0.37 -0.6 1,941,986 + clpB DVU1874 0.43 +0.0 1,942,033 - clpB DVU1874 0.45 +1.0 1,942,064 - clpB DVU1874 0.46 -1.1 1,942,092 - clpB DVU1874 0.47 -0.4 1,942,151 + clpB DVU1874 0.49 -2.7 1,942,183 + clpB DVU1874 0.51 -0.9 1,942,191 - clpB DVU1874 0.51 +1.4 1,942,191 - clpB DVU1874 0.51 +2.0 1,942,198 + clpB DVU1874 0.51 -2.6 1,942,198 + clpB DVU1874 0.51 -1.2 1,942,246 + clpB DVU1874 0.53 -0.8 1,942,246 + clpB DVU1874 0.53 -1.3 1,942,369 + clpB DVU1874 0.58 -1.0 1,942,463 + clpB DVU1874 0.61 +0.6 1,942,554 + clpB DVU1874 0.65 -1.2 1,942,701 + clpB DVU1874 0.70 +0.4 1,942,762 - clpB DVU1874 0.73 -1.3 1,942,783 + clpB DVU1874 0.74 -2.0 1,942,801 + clpB DVU1874 0.74 +1.4 1,942,802 - clpB DVU1874 0.74 -1.7 1,942,857 - clpB DVU1874 0.76 -1.5 1,942,857 - clpB DVU1874 0.76 +0.0 1,942,965 + clpB DVU1874 0.81 -1.5 1,943,039 - clpB DVU1874 0.83 -0.4 1,943,059 - clpB DVU1874 0.84 +0.0 1,943,102 - clpB DVU1874 0.86 +1.0 1,943,187 - clpB DVU1874 0.89 -0.5 1,943,431 - -1.9 1,943,600 - DVU1875 0.34 -0.2 1,943,643 - DVU1875 0.47 +0.3 1,943,796 + -0.2 1,943,809 + -0.5 1,943,834 + -0.3 1,943,879 + +1.4 1,943,887 - +0.1 1,943,888 + -1.6 1,943,974 + dnaJ DVU1876 0.14 +2.0 1,943,974 + dnaJ DVU1876 0.14 -2.4 1,943,974 + dnaJ DVU1876 0.14 -0.2 1,943,974 + dnaJ DVU1876 0.14 -1.3 1,943,982 - dnaJ DVU1876 0.15 -1.5 1,943,982 - dnaJ DVU1876 0.15 -1.3 1,944,060 + dnaJ DVU1876 0.23 -0.2 1,944,060 + dnaJ DVU1876 0.23 -1.8 1,944,067 + dnaJ DVU1876 0.24 -2.3 1,944,122 + dnaJ DVU1876 0.29 -0.6 1,944,172 + dnaJ DVU1876 0.35 -1.1 1,944,421 - dnaJ DVU1876 0.61 -0.9
Or see this region's nucleotide sequence