Experiment: MoYLS4 with FCCP 2uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobQ and DVU0817 are separated by 62 nucleotides DVU0817 and DVU0818 are separated by 202 nucleotides DVU0818 and isf-1 are separated by 313 nucleotides
DVU0816: cobQ - cobyric acid synthase CobQ (TIGR), at 904,464 to 906,095
cobQ
DVU0817: DVU0817 - hypothetical protein (TIGR), at 906,158 to 906,625
DVU0817
DVU0818: DVU0818 - conserved domain protein (TIGR), at 906,828 to 908,576
DVU0818
DVU0819: isf-1 - FMN reductase, NADPH-dependent (TIGR), at 908,890 to 909,507
isf-1
Position (kb) 906
907
908
909 Strain fitness (log2 ratio) -1
0
1
2 at 905.852 kb on - strand, within cobQ at 905.875 kb on - strand, within cobQ at 905.875 kb on - strand, within cobQ at 905.907 kb on + strand, within cobQ at 905.946 kb on - strand at 905.958 kb on - strand at 906.000 kb on + strand at 906.000 kb on + strand at 906.007 kb on + strand at 906.047 kb on + strand at 906.047 kb on + strand at 906.055 kb on - strand at 906.087 kb on - strand at 906.087 kb on - strand at 906.257 kb on + strand, within DVU0817 at 906.330 kb on + strand, within DVU0817 at 906.330 kb on + strand, within DVU0817 at 906.374 kb on - strand, within DVU0817 at 906.527 kb on - strand, within DVU0817 at 906.595 kb on - strand at 906.631 kb on - strand at 906.714 kb on + strand at 906.870 kb on - strand at 906.886 kb on + strand at 906.894 kb on - strand at 906.894 kb on - strand at 906.951 kb on + strand at 906.959 kb on - strand at 907.097 kb on - strand, within DVU0818 at 907.144 kb on + strand, within DVU0818 at 907.198 kb on + strand, within DVU0818 at 907.212 kb on - strand, within DVU0818 at 907.236 kb on - strand, within DVU0818 at 907.236 kb on - strand, within DVU0818 at 907.247 kb on - strand, within DVU0818 at 907.436 kb on + strand, within DVU0818 at 907.490 kb on - strand, within DVU0818 at 907.540 kb on - strand, within DVU0818 at 907.593 kb on + strand, within DVU0818 at 907.623 kb on + strand, within DVU0818 at 907.733 kb on - strand, within DVU0818 at 907.816 kb on + strand, within DVU0818 at 907.834 kb on + strand, within DVU0818 at 907.999 kb on + strand, within DVU0818 at 908.097 kb on + strand, within DVU0818 at 908.105 kb on - strand, within DVU0818 at 908.105 kb on - strand, within DVU0818 at 908.105 kb on - strand, within DVU0818 at 908.135 kb on - strand, within DVU0818 at 908.146 kb on + strand, within DVU0818 at 908.271 kb on + strand, within DVU0818 at 908.400 kb on - strand, within DVU0818 at 908.439 kb on + strand at 908.572 kb on + strand at 908.596 kb on - strand at 908.596 kb on - strand at 908.703 kb on + strand at 908.706 kb on - strand at 908.895 kb on - strand at 908.945 kb on + strand at 908.947 kb on - strand at 908.966 kb on + strand, within isf-1 at 909.033 kb on - strand, within isf-1 at 909.103 kb on + strand, within isf-1 at 909.215 kb on + strand, within isf-1 at 909.215 kb on + strand, within isf-1 at 909.223 kb on - strand, within isf-1 at 909.312 kb on - strand, within isf-1 at 909.378 kb on - strand, within isf-1 at 909.530 kb on - strand at 909.569 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with FCCP 2uM remove 905,852 - cobQ DVU0816 0.85 +0.9 905,875 - cobQ DVU0816 0.86 +0.0 905,875 - cobQ DVU0816 0.86 -0.8 905,907 + cobQ DVU0816 0.88 +0.5 905,946 - +0.4 905,958 - +0.4 906,000 + -0.4 906,000 + +1.0 906,007 + +0.6 906,047 + +0.1 906,047 + +1.1 906,055 - -0.6 906,087 - +1.0 906,087 - -1.2 906,257 + DVU0817 0.21 +0.0 906,330 + DVU0817 0.37 +0.3 906,330 + DVU0817 0.37 +1.0 906,374 - DVU0817 0.46 +0.2 906,527 - DVU0817 0.79 -1.6 906,595 - -0.5 906,631 - -1.0 906,714 + -0.2 906,870 - +2.2 906,886 + +0.6 906,894 - +0.0 906,894 - +1.4 906,951 + -1.6 906,959 - -0.2 907,097 - DVU0818 0.15 -0.6 907,144 + DVU0818 0.18 -0.6 907,198 + DVU0818 0.21 +1.0 907,212 - DVU0818 0.22 -0.1 907,236 - DVU0818 0.23 -0.4 907,236 - DVU0818 0.23 +0.4 907,247 - DVU0818 0.24 +0.0 907,436 + DVU0818 0.35 +0.5 907,490 - DVU0818 0.38 +0.3 907,540 - DVU0818 0.41 +0.4 907,593 + DVU0818 0.44 -0.8 907,623 + DVU0818 0.45 +0.4 907,733 - DVU0818 0.52 +2.0 907,816 + DVU0818 0.56 -0.3 907,834 + DVU0818 0.58 +0.2 907,999 + DVU0818 0.67 +0.0 908,097 + DVU0818 0.73 +1.0 908,105 - DVU0818 0.73 +0.9 908,105 - DVU0818 0.73 -0.9 908,105 - DVU0818 0.73 -0.7 908,135 - DVU0818 0.75 -0.1 908,146 + DVU0818 0.75 -0.2 908,271 + DVU0818 0.83 +0.3 908,400 - DVU0818 0.90 -0.6 908,439 + -0.6 908,572 + -0.4 908,596 - -0.3 908,596 - -0.1 908,703 + -1.4 908,706 - +0.1 908,895 - -0.4 908,945 + +0.0 908,947 - +1.0 908,966 + isf-1 DVU0819 0.12 -0.4 909,033 - isf-1 DVU0819 0.23 -0.2 909,103 + isf-1 DVU0819 0.34 -0.2 909,215 + isf-1 DVU0819 0.53 -0.2 909,215 + isf-1 DVU0819 0.53 -0.1 909,223 - isf-1 DVU0819 0.54 +0.3 909,312 - isf-1 DVU0819 0.68 -0.0 909,378 - isf-1 DVU0819 0.79 -0.5 909,530 - +0.0 909,569 + +0.7
Or see this region's nucleotide sequence