Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25115

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25100 and BWI76_RS25105 are separated by 206 nucleotidesBWI76_RS25105 and BWI76_RS25110 are separated by 130 nucleotidesBWI76_RS25110 and BWI76_RS25115 overlap by 21 nucleotidesBWI76_RS25115 and BWI76_RS25120 are separated by 153 nucleotidesBWI76_RS25120 and BWI76_RS25125 are separated by 14 nucleotidesBWI76_RS25125 and BWI76_RS25130 are separated by 229 nucleotides BWI76_RS25100: BWI76_RS25100 - ribosome maturation factor, at 5,226,910 to 5,227,362 _RS25100 BWI76_RS25105: BWI76_RS25105 - tRNA-Met, at 5,227,569 to 5,227,645 _RS25105 BWI76_RS25110: BWI76_RS25110 - hypothetical protein, at 5,227,776 to 5,227,973 _RS25110 BWI76_RS25115: BWI76_RS25115 - argininosuccinate synthase, at 5,227,953 to 5,229,296 _RS25115 BWI76_RS25120: BWI76_RS25120 - tRNA-Leu, at 5,229,450 to 5,229,536 _RS25120 BWI76_RS25125: BWI76_RS25125 - preprotein translocase subunit SecG, at 5,229,551 to 5,229,880 _RS25125 BWI76_RS25130: BWI76_RS25130 - phosphoglucosamine mutase, at 5,230,110 to 5,231,447 _RS25130 Position (kb) 5227 5228 5229 5230Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 5228.036 kb on + strandat 5228.036 kb on + strandat 5228.054 kb on - strandat 5228.077 kb on + strandat 5228.228 kb on - strand, within BWI76_RS25115at 5228.249 kb on + strand, within BWI76_RS25115at 5228.359 kb on + strand, within BWI76_RS25115at 5228.390 kb on + strand, within BWI76_RS25115at 5228.455 kb on + strand, within BWI76_RS25115at 5228.585 kb on - strand, within BWI76_RS25115at 5228.994 kb on + strand, within BWI76_RS25115at 5229.002 kb on - strand, within BWI76_RS25115at 5229.007 kb on - strand, within BWI76_RS25115at 5229.103 kb on - strand, within BWI76_RS25115at 5230.108 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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5,228,036 + -5.9
5,228,036 + -4.7
5,228,054 - -2.8
5,228,077 + -3.6
5,228,228 - BWI76_RS25115 0.20 -3.3
5,228,249 + BWI76_RS25115 0.22 -1.0
5,228,359 + BWI76_RS25115 0.30 -3.6
5,228,390 + BWI76_RS25115 0.33 -2.0
5,228,455 + BWI76_RS25115 0.37 -4.3
5,228,585 - BWI76_RS25115 0.47 -5.1
5,228,994 + BWI76_RS25115 0.77 -5.2
5,229,002 - BWI76_RS25115 0.78 -5.4
5,229,007 - BWI76_RS25115 0.78 -4.6
5,229,103 - BWI76_RS25115 0.86 -0.3
5,230,108 + -0.1

Or see this region's nucleotide sequence