Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21565

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21560 and BWI76_RS21565 are separated by 147 nucleotidesBWI76_RS21565 and BWI76_RS21570 are separated by 65 nucleotidesBWI76_RS21570 and BWI76_RS21575 overlap by 11 nucleotidesBWI76_RS21575 and BWI76_RS21580 are separated by 110 nucleotides BWI76_RS21560: BWI76_RS21560 - DNA repair protein RecN, at 4,513,052 to 4,514,713 _RS21560 BWI76_RS21565: BWI76_RS21565 - outer membrane protein assembly factor BamE, at 4,514,861 to 4,515,202 _RS21565 BWI76_RS21570: BWI76_RS21570 - RnfH family protein, at 4,515,268 to 4,515,558 _RS21570 BWI76_RS21575: BWI76_RS21575 - hypothetical protein, at 4,515,548 to 4,516,024 _RS21575 BWI76_RS21580: BWI76_RS21580 - SsrA-binding protein, at 4,516,135 to 4,516,617 _RS21580 Position (kb) 4514 4515 4516Strain fitness (log2 ratio) -1 0 1 2 3at 4514.133 kb on - strand, within BWI76_RS21560at 4514.506 kb on + strand, within BWI76_RS21560at 4514.549 kb on - strandat 4514.600 kb on + strandat 4514.822 kb on + strandat 4515.166 kb on - strand, within BWI76_RS21565at 4515.280 kb on + strandat 4515.361 kb on - strandat 4515.402 kb on + strand, within BWI76_RS21570at 4515.489 kb on + strand, within BWI76_RS21570at 4515.558 kb on + strandat 4515.566 kb on - strandat 4515.566 kb on - strandat 4516.070 kb on - strandat 4516.131 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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4,514,133 - BWI76_RS21560 0.65 +1.7
4,514,506 + BWI76_RS21560 0.87 -0.4
4,514,549 - -0.3
4,514,600 + -0.8
4,514,822 + +0.2
4,515,166 - BWI76_RS21565 0.89 -1.5
4,515,280 + -0.1
4,515,361 - +3.5
4,515,402 + BWI76_RS21570 0.46 +1.1
4,515,489 + BWI76_RS21570 0.76 -1.0
4,515,558 + -0.4
4,515,566 - -0.3
4,515,566 - -0.3
4,516,070 - -0.9
4,516,131 - -0.4

Or see this region's nucleotide sequence