Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05840

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05835 and BWI76_RS05840 are separated by 10 nucleotidesBWI76_RS05840 and BWI76_RS05845 are separated by 290 nucleotides BWI76_RS05835: BWI76_RS05835 - gamma-glutamyl-phosphate reductase, at 1,225,173 to 1,226,426 _RS05835 BWI76_RS05840: BWI76_RS05840 - glutamate 5-kinase, at 1,226,437 to 1,227,540 _RS05840 BWI76_RS05845: BWI76_RS05845 - phosphoporin PhoE, at 1,227,831 to 1,228,883 _RS05845 Position (kb) 1226 1227 1228Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1225.528 kb on - strand, within BWI76_RS05835at 1226.142 kb on + strand, within BWI76_RS05835at 1226.142 kb on + strand, within BWI76_RS05835at 1226.310 kb on + strandat 1226.318 kb on + strandat 1226.364 kb on + strandat 1226.501 kb on - strandat 1226.919 kb on - strand, within BWI76_RS05840at 1226.955 kb on + strand, within BWI76_RS05840at 1226.980 kb on - strand, within BWI76_RS05840at 1227.056 kb on - strand, within BWI76_RS05840at 1227.180 kb on + strand, within BWI76_RS05840at 1227.356 kb on - strand, within BWI76_RS05840at 1227.387 kb on + strand, within BWI76_RS05840at 1227.437 kb on - strandat 1227.469 kb on + strandat 1227.612 kb on + strandat 1227.612 kb on + strandat 1227.612 kb on + strandat 1227.630 kb on + strandat 1227.632 kb on + strandat 1227.638 kb on - strandat 1227.669 kb on + strandat 1227.767 kb on + strandat 1227.775 kb on - strandat 1227.775 kb on - strandat 1227.775 kb on - strandat 1227.787 kb on - strandat 1227.790 kb on + strandat 1227.798 kb on - strandat 1227.798 kb on - strandat 1227.798 kb on - strandat 1227.821 kb on + strandat 1227.821 kb on + strandat 1227.829 kb on - strandat 1227.853 kb on + strandat 1227.865 kb on + strandat 1227.938 kb on - strand, within BWI76_RS05845at 1227.941 kb on + strand, within BWI76_RS05845at 1227.949 kb on - strand, within BWI76_RS05845at 1227.949 kb on - strand, within BWI76_RS05845at 1227.949 kb on - strand, within BWI76_RS05845at 1227.954 kb on + strand, within BWI76_RS05845at 1227.956 kb on + strand, within BWI76_RS05845at 1227.964 kb on - strand, within BWI76_RS05845at 1227.964 kb on - strand, within BWI76_RS05845at 1227.987 kb on + strand, within BWI76_RS05845at 1227.995 kb on - strand, within BWI76_RS05845at 1228.000 kb on - strand, within BWI76_RS05845at 1228.127 kb on - strand, within BWI76_RS05845at 1228.199 kb on - strand, within BWI76_RS05845at 1228.253 kb on - strand, within BWI76_RS05845at 1228.321 kb on + strand, within BWI76_RS05845at 1228.409 kb on - strand, within BWI76_RS05845at 1228.446 kb on + strand, within BWI76_RS05845at 1228.446 kb on + strand, within BWI76_RS05845at 1228.446 kb on + strand, within BWI76_RS05845at 1228.446 kb on + strand, within BWI76_RS05845at 1228.454 kb on - strand, within BWI76_RS05845at 1228.528 kb on + strand, within BWI76_RS05845

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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1,225,528 - BWI76_RS05835 0.28 -4.2
1,226,142 + BWI76_RS05835 0.77 -3.6
1,226,142 + BWI76_RS05835 0.77 -2.2
1,226,310 + -4.2
1,226,318 + -4.0
1,226,364 + -0.7
1,226,501 - -2.2
1,226,919 - BWI76_RS05840 0.44 -4.6
1,226,955 + BWI76_RS05840 0.47 -3.8
1,226,980 - BWI76_RS05840 0.49 -4.0
1,227,056 - BWI76_RS05840 0.56 -1.1
1,227,180 + BWI76_RS05840 0.67 -3.0
1,227,356 - BWI76_RS05840 0.83 -2.7
1,227,387 + BWI76_RS05840 0.86 -2.4
1,227,437 - -4.0
1,227,469 + -4.5
1,227,612 + -0.1
1,227,612 + -0.4
1,227,612 + +1.4
1,227,630 + +0.2
1,227,632 + -1.5
1,227,638 - -0.0
1,227,669 + -0.5
1,227,767 + +0.2
1,227,775 - +0.2
1,227,775 - +0.3
1,227,775 - +0.0
1,227,787 - +1.9
1,227,790 + +0.0
1,227,798 - -0.8
1,227,798 - +1.1
1,227,798 - -0.2
1,227,821 + +0.4
1,227,821 + +1.0
1,227,829 - -0.3
1,227,853 + +0.2
1,227,865 + -1.1
1,227,938 - BWI76_RS05845 0.10 -0.5
1,227,941 + BWI76_RS05845 0.10 -0.3
1,227,949 - BWI76_RS05845 0.11 -0.7
1,227,949 - BWI76_RS05845 0.11 +2.3
1,227,949 - BWI76_RS05845 0.11 +0.8
1,227,954 + BWI76_RS05845 0.12 +0.3
1,227,956 + BWI76_RS05845 0.12 +0.3
1,227,964 - BWI76_RS05845 0.13 -0.7
1,227,964 - BWI76_RS05845 0.13 +0.5
1,227,987 + BWI76_RS05845 0.15 -0.7
1,227,995 - BWI76_RS05845 0.16 +1.1
1,228,000 - BWI76_RS05845 0.16 -1.1
1,228,127 - BWI76_RS05845 0.28 -0.7
1,228,199 - BWI76_RS05845 0.35 +0.3
1,228,253 - BWI76_RS05845 0.40 +0.2
1,228,321 + BWI76_RS05845 0.47 +0.2
1,228,409 - BWI76_RS05845 0.55 -1.8
1,228,446 + BWI76_RS05845 0.58 -0.1
1,228,446 + BWI76_RS05845 0.58 +0.1
1,228,446 + BWI76_RS05845 0.58 +1.1
1,228,446 + BWI76_RS05845 0.58 +0.1
1,228,454 - BWI76_RS05845 0.59 -1.1
1,228,528 + BWI76_RS05845 0.66 -1.0

Or see this region's nucleotide sequence