Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04675

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04670 and BWI76_RS04675 are separated by 2 nucleotidesBWI76_RS04675 and BWI76_RS04680 overlap by 1 nucleotides BWI76_RS04670: BWI76_RS04670 - 3-isopropylmalate dehydratase large subunit, at 973,685 to 975,085 _RS04670 BWI76_RS04675: BWI76_RS04675 - 3-isopropylmalate dehydrogenase, at 975,088 to 976,179 _RS04675 BWI76_RS04680: BWI76_RS04680 - 2-isopropylmalate synthase, at 976,179 to 977,750 _RS04680 Position (kb) 975 976 977Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 974.120 kb on + strand, within BWI76_RS04670at 974.272 kb on - strand, within BWI76_RS04670at 974.372 kb on + strand, within BWI76_RS04670at 974.395 kb on - strand, within BWI76_RS04670at 974.433 kb on + strand, within BWI76_RS04670at 974.439 kb on - strand, within BWI76_RS04670at 974.477 kb on + strand, within BWI76_RS04670at 974.480 kb on - strand, within BWI76_RS04670at 974.741 kb on - strandat 974.797 kb on + strand, within BWI76_RS04670at 974.894 kb on + strand, within BWI76_RS04670at 974.924 kb on - strand, within BWI76_RS04670at 974.924 kb on - strand, within BWI76_RS04670at 975.006 kb on + strandat 975.145 kb on - strandat 975.438 kb on + strand, within BWI76_RS04675at 975.473 kb on + strand, within BWI76_RS04675at 975.788 kb on - strand, within BWI76_RS04675at 975.833 kb on - strand, within BWI76_RS04675at 975.841 kb on - strand, within BWI76_RS04675at 975.899 kb on + strand, within BWI76_RS04675at 975.900 kb on - strand, within BWI76_RS04675at 976.189 kb on + strandat 976.321 kb on - strandat 976.321 kb on - strandat 976.368 kb on - strand, within BWI76_RS04680at 976.375 kb on + strand, within BWI76_RS04680at 976.383 kb on - strand, within BWI76_RS04680at 976.388 kb on - strand, within BWI76_RS04680at 976.423 kb on - strand, within BWI76_RS04680at 976.517 kb on + strand, within BWI76_RS04680at 976.628 kb on + strand, within BWI76_RS04680at 976.696 kb on - strand, within BWI76_RS04680at 976.775 kb on + strand, within BWI76_RS04680at 976.807 kb on - strand, within BWI76_RS04680at 976.858 kb on + strand, within BWI76_RS04680at 976.895 kb on - strand, within BWI76_RS04680at 976.918 kb on + strand, within BWI76_RS04680at 977.079 kb on - strand, within BWI76_RS04680at 977.084 kb on - strand, within BWI76_RS04680at 977.091 kb on + strand, within BWI76_RS04680at 977.104 kb on - strand, within BWI76_RS04680at 977.135 kb on - strand, within BWI76_RS04680at 977.172 kb on + strand, within BWI76_RS04680

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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974,120 + BWI76_RS04670 0.31 -5.0
974,272 - BWI76_RS04670 0.42 -3.8
974,372 + BWI76_RS04670 0.49 -3.0
974,395 - BWI76_RS04670 0.51 -0.9
974,433 + BWI76_RS04670 0.53 -3.9
974,439 - BWI76_RS04670 0.54 -4.8
974,477 + BWI76_RS04670 0.57 -3.3
974,480 - BWI76_RS04670 0.57 -4.3
974,741 - +0.2
974,797 + BWI76_RS04670 0.79 -2.1
974,894 + BWI76_RS04670 0.86 -5.8
974,924 - BWI76_RS04670 0.88 -4.0
974,924 - BWI76_RS04670 0.88 -1.4
975,006 + -0.4
975,145 - -1.2
975,438 + BWI76_RS04675 0.32 -3.5
975,473 + BWI76_RS04675 0.35 -3.5
975,788 - BWI76_RS04675 0.64 -5.6
975,833 - BWI76_RS04675 0.68 -4.5
975,841 - BWI76_RS04675 0.69 -2.6
975,899 + BWI76_RS04675 0.74 -4.6
975,900 - BWI76_RS04675 0.74 -3.4
976,189 + -5.8
976,321 - -2.9
976,321 - -4.2
976,368 - BWI76_RS04680 0.12 -3.7
976,375 + BWI76_RS04680 0.12 -5.6
976,383 - BWI76_RS04680 0.13 -2.2
976,388 - BWI76_RS04680 0.13 +0.6
976,423 - BWI76_RS04680 0.16 -1.9
976,517 + BWI76_RS04680 0.22 -3.1
976,628 + BWI76_RS04680 0.29 -4.0
976,696 - BWI76_RS04680 0.33 -4.5
976,775 + BWI76_RS04680 0.38 -5.7
976,807 - BWI76_RS04680 0.40 -3.6
976,858 + BWI76_RS04680 0.43 -3.6
976,895 - BWI76_RS04680 0.46 -4.4
976,918 + BWI76_RS04680 0.47 -0.0
977,079 - BWI76_RS04680 0.57 -0.8
977,084 - BWI76_RS04680 0.58 -4.3
977,091 + BWI76_RS04680 0.58 -2.7
977,104 - BWI76_RS04680 0.59 -5.8
977,135 - BWI76_RS04680 0.61 +1.0
977,172 + BWI76_RS04680 0.63 -3.3

Or see this region's nucleotide sequence