Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04250

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04245 and BWI76_RS04250 are separated by 1 nucleotidesBWI76_RS04250 and BWI76_RS04255 are separated by 3 nucleotides BWI76_RS04245: BWI76_RS04245 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 872,525 to 874,987 _RS04245 BWI76_RS04250: BWI76_RS04250 - homoserine kinase, at 874,989 to 875,918 _RS04250 BWI76_RS04255: BWI76_RS04255 - threonine synthase, at 875,922 to 877,202 _RS04255 Position (kb) 874 875 876Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 874.039 kb on + strand, within BWI76_RS04245at 874.039 kb on + strand, within BWI76_RS04245at 874.082 kb on - strand, within BWI76_RS04245at 874.282 kb on + strand, within BWI76_RS04245at 874.290 kb on + strand, within BWI76_RS04245at 874.435 kb on - strand, within BWI76_RS04245at 874.480 kb on - strand, within BWI76_RS04245at 874.490 kb on - strand, within BWI76_RS04245at 874.654 kb on - strand, within BWI76_RS04245at 874.675 kb on - strand, within BWI76_RS04245at 874.905 kb on + strandat 874.949 kb on + strandat 875.101 kb on - strand, within BWI76_RS04250at 875.266 kb on + strand, within BWI76_RS04250at 875.274 kb on - strand, within BWI76_RS04250at 875.422 kb on - strand, within BWI76_RS04250at 875.516 kb on - strand, within BWI76_RS04250at 875.539 kb on - strandat 875.587 kb on + strand, within BWI76_RS04250at 875.595 kb on - strand, within BWI76_RS04250at 875.599 kb on + strand, within BWI76_RS04250at 875.615 kb on - strand, within BWI76_RS04250at 875.846 kb on - strandat 875.962 kb on + strandat 875.965 kb on - strandat 875.970 kb on - strandat 875.987 kb on - strandat 876.169 kb on - strand, within BWI76_RS04255at 876.213 kb on - strand, within BWI76_RS04255at 876.223 kb on + strand, within BWI76_RS04255at 876.398 kb on + strand, within BWI76_RS04255at 876.428 kb on + strand, within BWI76_RS04255at 876.620 kb on + strand, within BWI76_RS04255at 876.620 kb on + strand, within BWI76_RS04255at 876.644 kb on + strand, within BWI76_RS04255at 876.644 kb on + strand, within BWI76_RS04255at 876.812 kb on + strand, within BWI76_RS04255at 876.812 kb on + strand, within BWI76_RS04255at 876.820 kb on - strand, within BWI76_RS04255at 876.820 kb on - strand, within BWI76_RS04255

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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874,039 + BWI76_RS04245 0.61 -3.7
874,039 + BWI76_RS04245 0.61 -5.9
874,082 - BWI76_RS04245 0.63 -2.5
874,282 + BWI76_RS04245 0.71 -4.4
874,290 + BWI76_RS04245 0.72 -2.5
874,435 - BWI76_RS04245 0.78 -4.1
874,480 - BWI76_RS04245 0.79 -6.0
874,490 - BWI76_RS04245 0.80 -1.7
874,654 - BWI76_RS04245 0.86 -3.3
874,675 - BWI76_RS04245 0.87 -3.9
874,905 + -3.6
874,949 + -2.5
875,101 - BWI76_RS04250 0.12 -4.4
875,266 + BWI76_RS04250 0.30 -2.2
875,274 - BWI76_RS04250 0.31 -4.1
875,422 - BWI76_RS04250 0.47 -6.1
875,516 - BWI76_RS04250 0.57 -4.5
875,539 - +0.0
875,587 + BWI76_RS04250 0.64 -3.6
875,595 - BWI76_RS04250 0.65 -3.4
875,599 + BWI76_RS04250 0.66 -4.0
875,615 - BWI76_RS04250 0.67 -1.8
875,846 - -4.7
875,962 + -4.4
875,965 - -2.9
875,970 - -3.1
875,987 - -5.3
876,169 - BWI76_RS04255 0.19 -4.0
876,213 - BWI76_RS04255 0.23 -2.4
876,223 + BWI76_RS04255 0.23 -5.9
876,398 + BWI76_RS04255 0.37 -2.3
876,428 + BWI76_RS04255 0.40 -1.6
876,620 + BWI76_RS04255 0.54 -5.1
876,620 + BWI76_RS04255 0.54 -3.0
876,644 + BWI76_RS04255 0.56 -4.0
876,644 + BWI76_RS04255 0.56 -0.8
876,812 + BWI76_RS04255 0.69 -6.3
876,812 + BWI76_RS04255 0.69 -5.1
876,820 - BWI76_RS04255 0.70 +0.4
876,820 - BWI76_RS04255 0.70 -4.1

Or see this region's nucleotide sequence