Experiment: L-Glutamic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00975 and BWI76_RS00980 are separated by 2 nucleotides BWI76_RS00980 and BWI76_RS00985 are separated by 58 nucleotides
BWI76_RS00975: BWI76_RS00975 - dihydroxy-acid dehydratase, at 198,294 to 200,144
_RS00975
BWI76_RS00980: BWI76_RS00980 - PLP-dependent threonine dehydratase, at 200,147 to 201,691
_RS00980
BWI76_RS00985: BWI76_RS00985 - transcriptional regulator IlvY, at 201,750 to 202,640
_RS00985
Position (kb) 200
201
202 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.234 kb on - strand, within BWI76_RS00975 at 199.234 kb on - strand, within BWI76_RS00975 at 199.740 kb on - strand, within BWI76_RS00975 at 199.806 kb on + strand, within BWI76_RS00975 at 199.877 kb on - strand, within BWI76_RS00975 at 199.960 kb on - strand at 200.009 kb on + strand at 200.062 kb on + strand at 200.070 kb on - strand at 200.192 kb on - strand at 200.207 kb on - strand at 200.207 kb on - strand at 200.229 kb on - strand at 200.244 kb on - strand at 200.325 kb on + strand, within BWI76_RS00980 at 200.325 kb on + strand, within BWI76_RS00980 at 200.325 kb on + strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.558 kb on + strand, within BWI76_RS00980 at 200.652 kb on - strand, within BWI76_RS00980 at 200.792 kb on + strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.869 kb on - strand, within BWI76_RS00980 at 200.929 kb on + strand, within BWI76_RS00980 at 201.380 kb on - strand, within BWI76_RS00980 at 201.454 kb on - strand at 201.505 kb on + strand, within BWI76_RS00980 at 201.565 kb on - strand at 201.629 kb on - strand at 201.918 kb on - strand, within BWI76_RS00985 at 201.928 kb on - strand, within BWI76_RS00985 at 201.974 kb on + strand, within BWI76_RS00985 at 202.030 kb on - strand, within BWI76_RS00985 at 202.043 kb on - strand, within BWI76_RS00985 at 202.101 kb on - strand, within BWI76_RS00985 at 202.114 kb on + strand, within BWI76_RS00985 at 202.114 kb on + strand, within BWI76_RS00985 at 202.177 kb on + strand, within BWI76_RS00985 at 202.264 kb on + strand, within BWI76_RS00985 at 202.444 kb on + strand, within BWI76_RS00985 at 202.515 kb on + strand, within BWI76_RS00985 at 202.560 kb on + strand at 202.610 kb on + strand at 202.625 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (N) remove 199,226 + BWI76_RS00975 0.50 -6.0 199,226 + BWI76_RS00975 0.50 -0.7 199,226 + BWI76_RS00975 0.50 -5.1 199,226 + BWI76_RS00975 0.50 -1.3 199,234 - BWI76_RS00975 0.51 -1.8 199,234 - BWI76_RS00975 0.51 -5.0 199,740 - BWI76_RS00975 0.78 -0.9 199,806 + BWI76_RS00975 0.82 -1.9 199,877 - BWI76_RS00975 0.86 -3.7 199,960 - -3.1 200,009 + -2.1 200,062 + +0.1 200,070 - -3.7 200,192 - -0.3 200,207 - -3.8 200,207 - -2.7 200,229 - -2.7 200,244 - -3.9 200,325 + BWI76_RS00980 0.12 -5.7 200,325 + BWI76_RS00980 0.12 -2.7 200,325 + BWI76_RS00980 0.12 -4.9 200,333 - BWI76_RS00980 0.12 -3.3 200,333 - BWI76_RS00980 0.12 -3.4 200,333 - BWI76_RS00980 0.12 -1.6 200,333 - BWI76_RS00980 0.12 -2.7 200,333 - BWI76_RS00980 0.12 -3.3 200,333 - BWI76_RS00980 0.12 -2.6 200,333 - BWI76_RS00980 0.12 -3.1 200,333 - BWI76_RS00980 0.12 -2.4 200,333 - BWI76_RS00980 0.12 -4.7 200,333 - BWI76_RS00980 0.12 -3.2 200,558 + BWI76_RS00980 0.27 -5.6 200,652 - BWI76_RS00980 0.33 -2.3 200,792 + BWI76_RS00980 0.42 -3.4 200,845 - BWI76_RS00980 0.45 +0.6 200,845 - BWI76_RS00980 0.45 -2.5 200,869 - BWI76_RS00980 0.47 -2.9 200,929 + BWI76_RS00980 0.51 -4.9 201,380 - BWI76_RS00980 0.80 -0.4 201,454 - +0.1 201,505 + BWI76_RS00980 0.88 -3.7 201,565 - -4.3 201,629 - -2.1 201,918 - BWI76_RS00985 0.19 -1.8 201,928 - BWI76_RS00985 0.20 -2.4 201,974 + BWI76_RS00985 0.25 -0.6 202,030 - BWI76_RS00985 0.31 -2.1 202,043 - BWI76_RS00985 0.33 -1.8 202,101 - BWI76_RS00985 0.39 -2.7 202,114 + BWI76_RS00985 0.41 -1.0 202,114 + BWI76_RS00985 0.41 -0.3 202,177 + BWI76_RS00985 0.48 -1.2 202,264 + BWI76_RS00985 0.58 -0.9 202,444 + BWI76_RS00985 0.78 -0.3 202,515 + BWI76_RS00985 0.86 -2.6 202,560 + -2.4 202,610 + -0.0 202,625 - -1.3
Or see this region's nucleotide sequence