Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00970

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00960 and BWI76_RS00965 overlap by 4 nucleotidesBWI76_RS00965 and BWI76_RS00970 are separated by 16 nucleotidesBWI76_RS00970 and BWI76_RS00975 are separated by 60 nucleotides BWI76_RS00960: BWI76_RS00960 - acetolactate synthase 2 catalytic subunit, at 195,387 to 197,033 _RS00960 BWI76_RS00965: BWI76_RS00965 - acetolactate synthase 2 small subunit, at 197,030 to 197,287 _RS00965 BWI76_RS00970: BWI76_RS00970 - branched chain amino acid aminotransferase, at 197,304 to 198,233 _RS00970 BWI76_RS00975: BWI76_RS00975 - dihydroxy-acid dehydratase, at 198,294 to 200,144 _RS00975 Position (kb) 197 198 199Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 196.541 kb on - strand, within BWI76_RS00960at 196.597 kb on + strandat 196.605 kb on - strand, within BWI76_RS00960at 196.754 kb on + strand, within BWI76_RS00960at 196.869 kb on + strandat 196.933 kb on + strandat 196.941 kb on - strandat 196.973 kb on + strandat 196.981 kb on - strandat 197.236 kb on - strand, within BWI76_RS00965at 197.330 kb on - strandat 197.383 kb on + strandat 197.399 kb on - strand, within BWI76_RS00970at 197.484 kb on + strand, within BWI76_RS00970at 197.484 kb on + strand, within BWI76_RS00970at 197.540 kb on + strand, within BWI76_RS00970at 197.540 kb on + strand, within BWI76_RS00970at 197.686 kb on + strand, within BWI76_RS00970at 198.044 kb on + strand, within BWI76_RS00970at 198.112 kb on + strand, within BWI76_RS00970at 198.168 kb on + strandat 198.297 kb on + strandat 198.442 kb on + strandat 198.442 kb on + strandat 198.543 kb on + strand, within BWI76_RS00975at 198.551 kb on - strand, within BWI76_RS00975at 198.608 kb on + strand, within BWI76_RS00975at 198.636 kb on - strand, within BWI76_RS00975at 198.648 kb on + strand, within BWI76_RS00975at 198.849 kb on + strand, within BWI76_RS00975at 198.850 kb on - strand, within BWI76_RS00975at 198.850 kb on - strand, within BWI76_RS00975at 199.017 kb on - strand, within BWI76_RS00975at 199.040 kb on + strand, within BWI76_RS00975at 199.043 kb on - strand, within BWI76_RS00975at 199.226 kb on + strand, within BWI76_RS00975at 199.226 kb on + strand, within BWI76_RS00975at 199.226 kb on + strand, within BWI76_RS00975at 199.226 kb on + strand, within BWI76_RS00975

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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196,541 - BWI76_RS00960 0.70 -0.6
196,597 + +1.1
196,605 - BWI76_RS00960 0.74 -0.9
196,754 + BWI76_RS00960 0.83 -0.7
196,869 + -0.1
196,933 + -0.7
196,941 - -1.1
196,973 + -0.0
196,981 - -2.2
197,236 - BWI76_RS00965 0.80 -4.1
197,330 - -1.8
197,383 + +0.6
197,399 - BWI76_RS00970 0.10 -4.2
197,484 + BWI76_RS00970 0.19 -0.5
197,484 + BWI76_RS00970 0.19 -0.2
197,540 + BWI76_RS00970 0.25 -1.1
197,540 + BWI76_RS00970 0.25 -2.3
197,686 + BWI76_RS00970 0.41 -0.2
198,044 + BWI76_RS00970 0.80 -0.4
198,112 + BWI76_RS00970 0.87 -0.1
198,168 + +0.2
198,297 + -5.2
198,442 + -2.4
198,442 + -4.0
198,543 + BWI76_RS00975 0.13 -2.4
198,551 - BWI76_RS00975 0.14 -2.6
198,608 + BWI76_RS00975 0.17 -2.6
198,636 - BWI76_RS00975 0.18 -5.7
198,648 + BWI76_RS00975 0.19 -2.9
198,849 + BWI76_RS00975 0.30 -1.4
198,850 - BWI76_RS00975 0.30 -5.0
198,850 - BWI76_RS00975 0.30 -4.5
199,017 - BWI76_RS00975 0.39 -6.8
199,040 + BWI76_RS00975 0.40 +0.6
199,043 - BWI76_RS00975 0.40 -4.7
199,226 + BWI76_RS00975 0.50 -6.0
199,226 + BWI76_RS00975 0.50 -0.7
199,226 + BWI76_RS00975 0.50 -5.1
199,226 + BWI76_RS00975 0.50 -1.3

Or see this region's nucleotide sequence