Experiment: m.b. Cephalothin 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3091 and Dshi_3092 overlap by 4 nucleotides Dshi_3092 and Dshi_3093 overlap by 4 nucleotides Dshi_3093 and Dshi_3094 overlap by 1 nucleotides Dshi_3094 and Dshi_3095 overlap by 1 nucleotides Dshi_3095 and Dshi_3096 are separated by 2 nucleotides
Dshi_3091: Dshi_3091 - aspartate carbamoyltransferase (RefSeq), at 3,260,134 to 3,261,084
_3091
Dshi_3092: Dshi_3092 - hypothetical protein (RefSeq), at 3,261,081 to 3,261,506
_3092
Dshi_3093: Dshi_3093 - hypothetical protein (RefSeq), at 3,261,503 to 3,262,081
_3093
Dshi_3094: Dshi_3094 - dihydroorotase, multifunctional complex type (RefSeq), at 3,262,081 to 3,263,379
_3094
Dshi_3095: Dshi_3095 - protein of unknown function DUF205 (RefSeq), at 3,263,379 to 3,263,990
_3095
Dshi_3096: Dshi_3096 - protein of unknown function DUF805 (RefSeq), at 3,263,993 to 3,264,499
_3096
Position (kb)
3262
3263
3264 Strain fitness (log2 ratio)
-2
-1
0
1 at 3261.093 kb on - strand at 3261.237 kb on - strand, within Dshi_3092 at 3261.285 kb on - strand, within Dshi_3092 at 3261.359 kb on + strand, within Dshi_3092 at 3261.422 kb on + strand, within Dshi_3092 at 3261.589 kb on - strand, within Dshi_3093 at 3262.039 kb on - strand at 3262.209 kb on + strand at 3262.440 kb on + strand, within Dshi_3094 at 3262.527 kb on + strand, within Dshi_3094 at 3263.344 kb on - strand at 3263.998 kb on - strand at 3264.004 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cephalothin 0.0001 mg/ml remove 3,261,093 - -0.2 3,261,237 - Dshi_3092 0.37 -1.5 3,261,285 - Dshi_3092 0.48 -0.4 3,261,359 + Dshi_3092 0.65 -0.2 3,261,422 + Dshi_3092 0.80 -2.1 3,261,589 - Dshi_3093 0.15 -0.3 3,262,039 - -1.6 3,262,209 + -0.7 3,262,440 + Dshi_3094 0.28 -0.4 3,262,527 + Dshi_3094 0.34 -0.6 3,263,344 - -0.9 3,263,998 - +0.5 3,264,004 - -0.1
Or see this region's nucleotide sequence