Experiment: m.b. Cephalothin 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2398 and Dshi_2399 are separated by 10 nucleotides Dshi_2399 and Dshi_2400 are separated by 90 nucleotides Dshi_2400 and Dshi_2401 are separated by 175 nucleotides
Dshi_2398: Dshi_2398 - dihydropteroate synthase (RefSeq), at 2,532,406 to 2,533,419
_2398
Dshi_2399: Dshi_2399 - dihydroneopterin aldolase (RefSeq), at 2,533,430 to 2,534,281
_2399
Dshi_2400: Dshi_2400 - cell wall hydrolase SleB (RefSeq), at 2,534,372 to 2,535,064
_2400
Dshi_2401: Dshi_2401 - PEP-utilizing protein (RefSeq), at 2,535,240 to 2,537,828
_2401
Position (kb)
2534
2535
2536 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2534.402 kb on - strand at 2534.642 kb on - strand, within Dshi_2400 at 2534.787 kb on - strand, within Dshi_2400 at 2534.856 kb on - strand, within Dshi_2400 at 2534.894 kb on - strand, within Dshi_2400 at 2534.919 kb on + strand, within Dshi_2400 at 2534.946 kb on - strand, within Dshi_2400 at 2534.946 kb on - strand, within Dshi_2400 at 2535.082 kb on - strand at 2535.471 kb on - strand at 2535.669 kb on + strand, within Dshi_2401 at 2535.769 kb on + strand, within Dshi_2401 at 2535.838 kb on + strand, within Dshi_2401 at 2535.918 kb on - strand, within Dshi_2401 at 2535.974 kb on + strand, within Dshi_2401
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cephalothin 0.0001 mg/ml remove 2,534,402 - -0.4 2,534,642 - Dshi_2400 0.39 -2.7 2,534,787 - Dshi_2400 0.60 -0.0 2,534,856 - Dshi_2400 0.70 +0.1 2,534,894 - Dshi_2400 0.75 -1.0 2,534,919 + Dshi_2400 0.79 -0.2 2,534,946 - Dshi_2400 0.83 -0.6 2,534,946 - Dshi_2400 0.83 -0.8 2,535,082 - -0.0 2,535,471 - -1.4 2,535,669 + Dshi_2401 0.17 +0.8 2,535,769 + Dshi_2401 0.20 -0.4 2,535,838 + Dshi_2401 0.23 +0.4 2,535,918 - Dshi_2401 0.26 +0.1 2,535,974 + Dshi_2401 0.28 +1.9
Or see this region's nucleotide sequence