Experiment: m.b. Cephalothin 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1031 and Dshi_1032 are separated by 224 nucleotides Dshi_1032 and Dshi_1033 are separated by 81 nucleotides Dshi_1033 and Dshi_1034 overlap by 8 nucleotides Dshi_1034 and Dshi_1035 are separated by 385 nucleotides
Dshi_1031: Dshi_1031 - tryptophan synthase, beta subunit (RefSeq), at 1,064,459 to 1,065,691
_1031
Dshi_1032: Dshi_1032 - hypothetical protein (RefSeq), at 1,065,916 to 1,066,362
_1032
Dshi_1033: Dshi_1033 - hypothetical protein (RefSeq), at 1,066,444 to 1,066,914
_1033
Dshi_1034: Dshi_1034 - transcriptional regulator, LuxR family (RefSeq), at 1,066,907 to 1,067,449
_1034
Dshi_1035: Dshi_1035 - TRAP dicarboxylate transporter- DctP subunit (RefSeq), at 1,067,835 to 1,068,809
_1035
Position (kb)
1066
1067 Strain fitness (log2 ratio)
-1
0
1 at 1065.951 kb on + strand at 1065.967 kb on + strand, within Dshi_1032 at 1066.091 kb on + strand, within Dshi_1032 at 1066.095 kb on + strand, within Dshi_1032 at 1066.355 kb on - strand at 1066.534 kb on + strand, within Dshi_1033 at 1066.542 kb on - strand, within Dshi_1033 at 1066.599 kb on + strand, within Dshi_1033 at 1067.089 kb on + strand, within Dshi_1034 at 1067.234 kb on + strand, within Dshi_1034 at 1067.273 kb on - strand, within Dshi_1034 at 1067.354 kb on - strand, within Dshi_1034 at 1067.435 kb on + strand at 1067.506 kb on - strand at 1067.506 kb on - strand at 1067.753 kb on - strand at 1067.753 kb on - strand at 1067.871 kb on - strand at 1067.886 kb on - strand at 1067.904 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cephalothin 0.0001 mg/ml remove 1,065,951 + -0.1 1,065,967 + Dshi_1032 0.11 +0.1 1,066,091 + Dshi_1032 0.39 +0.6 1,066,095 + Dshi_1032 0.40 -1.0 1,066,355 - +0.1 1,066,534 + Dshi_1033 0.19 -0.4 1,066,542 - Dshi_1033 0.21 -0.6 1,066,599 + Dshi_1033 0.33 -1.1 1,067,089 + Dshi_1034 0.34 +0.4 1,067,234 + Dshi_1034 0.60 -0.1 1,067,273 - Dshi_1034 0.67 -0.1 1,067,354 - Dshi_1034 0.82 -1.3 1,067,435 + +0.3 1,067,506 - +1.4 1,067,506 - -0.1 1,067,753 - -0.4 1,067,753 - +0.5 1,067,871 - -1.3 1,067,886 - -0.4 1,067,904 - +0.8
Or see this region's nucleotide sequence