Experiment: m.b. Cephalothin 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0324 and Dshi_0325 are separated by 87 nucleotides Dshi_0325 and Dshi_0326 are separated by 62 nucleotides Dshi_0326 and Dshi_0327 overlap by 4 nucleotides Dshi_0327 and Dshi_0328 overlap by 4 nucleotides
Dshi_0324: Dshi_0324 - acetylglutamate kinase (RefSeq), at 311,973 to 312,836
_0324
Dshi_0325: Dshi_0325 - short-chain dehydrogenase/reductase SDR (RefSeq), at 312,924 to 313,655
_0325
Dshi_0326: Dshi_0326 - GTP-binding protein HSR1-related (RefSeq), at 313,718 to 314,371
_0326
Dshi_0327: Dshi_0327 - MOSC domain containing protein (RefSeq), at 314,368 to 315,117
_0327
Dshi_0328: Dshi_0328 - 60 kDa inner membrane insertion protein (RefSeq), at 315,114 to 316,934
_0328
Position (kb) 313
314
315 Strain fitness (log2 ratio) -1
0
1 at 312.893 kb on + strand at 312.893 kb on + strand at 312.901 kb on - strand at 312.901 kb on - strand at 312.901 kb on - strand at 312.901 kb on - strand at 312.937 kb on - strand at 313.027 kb on + strand, within Dshi_0325 at 313.027 kb on - strand, within Dshi_0325 at 313.187 kb on + strand, within Dshi_0325 at 313.343 kb on - strand, within Dshi_0325 at 313.356 kb on + strand, within Dshi_0325 at 313.370 kb on - strand, within Dshi_0325 at 313.383 kb on + strand, within Dshi_0325
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cephalothin 0.0001 mg/ml remove 312,893 + +1.2 312,893 + -1.1 312,901 - +1.3 312,901 - +0.6 312,901 - -1.2 312,901 - -1.1 312,937 - +0.9 313,027 + Dshi_0325 0.14 -1.2 313,027 - Dshi_0325 0.14 +0.3 313,187 + Dshi_0325 0.36 -0.5 313,343 - Dshi_0325 0.57 -1.0 313,356 + Dshi_0325 0.59 -0.6 313,370 - Dshi_0325 0.61 -1.2 313,383 + Dshi_0325 0.63 -0.8
Or see this region's nucleotide sequence