Experiment: Pyruvate-Thiosulfate (60-15 mM) + 0.1% Yeast Extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2149 and dksA are separated by 19 nucleotides dksA and tRNA-Gly are separated by 745 nucleotides tRNA-Gly and tRNA-Cys are separated by 11 nucleotides tRNA-Cys and tRNA-Gly are separated by 7 nucleotides
DVU2149: DVU2149 - conserved hypothetical protein (TIGR), at 2,247,518 to 2,249,257
DVU2149
DVU2150: dksA - dnaK suppressor protein, putative (TIGR), at 2,249,277 to 2,249,639
dksA
DVU_tRNA-Gly-1: tRNA-Gly - tRNA with anticodon GCC for Gly (VIMSS-AUTO), at 2,250,385 to 2,250,459
tRNA-Gly
DVU_tRNA-Cys-1: tRNA-Cys - tRNA with anticodon GCA for Cys (VIMSS-AUTO), at 2,250,471 to 2,250,545
tRNA-Cys
DVU_tRNA-Gly-2: tRNA-Gly - tRNA with anticodon GCC for Gly (VIMSS-AUTO), at 2,250,553 to 2,250,627
tRNA-Gly
Position (kb)
2249
2250 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2248.277 kb on - strand, within DVU2149 at 2248.328 kb on - strand, within DVU2149 at 2248.390 kb on - strand, within DVU2149 at 2248.422 kb on + strand, within DVU2149 at 2248.473 kb on + strand, within DVU2149 at 2248.473 kb on + strand, within DVU2149 at 2248.484 kb on + strand, within DVU2149 at 2248.564 kb on + strand, within DVU2149 at 2248.571 kb on + strand, within DVU2149 at 2248.572 kb on - strand, within DVU2149 at 2248.572 kb on - strand, within DVU2149 at 2248.576 kb on + strand, within DVU2149 at 2248.579 kb on - strand, within DVU2149 at 2248.579 kb on - strand, within DVU2149 at 2248.584 kb on - strand, within DVU2149 at 2248.588 kb on + strand, within DVU2149 at 2248.595 kb on + strand, within DVU2149 at 2248.596 kb on - strand, within DVU2149 at 2248.718 kb on - strand, within DVU2149 at 2248.722 kb on + strand, within DVU2149 at 2248.793 kb on + strand, within DVU2149 at 2248.830 kb on - strand, within DVU2149 at 2248.843 kb on + strand, within DVU2149 at 2248.928 kb on - strand, within DVU2149 at 2248.968 kb on - strand, within DVU2149 at 2249.010 kb on + strand, within DVU2149 at 2249.037 kb on - strand, within DVU2149 at 2249.092 kb on + strand at 2249.154 kb on + strand at 2249.216 kb on + strand at 2249.282 kb on - strand at 2249.347 kb on - strand, within dksA at 2249.454 kb on + strand, within dksA at 2249.550 kb on + strand, within dksA at 2249.558 kb on - strand, within dksA at 2249.606 kb on - strand at 2249.863 kb on + strand at 2249.871 kb on - strand at 2249.871 kb on - strand at 2249.898 kb on + strand at 2249.903 kb on + strand at 2250.167 kb on + strand at 2250.188 kb on + strand at 2250.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Thiosulfate (60-15 mM) + 0.1% Yeast Extract remove 2,248,277 - DVU2149 0.44 +0.8 2,248,328 - DVU2149 0.47 +0.2 2,248,390 - DVU2149 0.50 -0.1 2,248,422 + DVU2149 0.52 -1.3 2,248,473 + DVU2149 0.55 +0.5 2,248,473 + DVU2149 0.55 +0.1 2,248,484 + DVU2149 0.56 +1.0 2,248,564 + DVU2149 0.60 -1.0 2,248,571 + DVU2149 0.61 +0.3 2,248,572 - DVU2149 0.61 -0.4 2,248,572 - DVU2149 0.61 -0.3 2,248,576 + DVU2149 0.61 -0.8 2,248,579 - DVU2149 0.61 -2.0 2,248,579 - DVU2149 0.61 +0.3 2,248,584 - DVU2149 0.61 +0.9 2,248,588 + DVU2149 0.61 +2.0 2,248,595 + DVU2149 0.62 +0.4 2,248,596 - DVU2149 0.62 -0.1 2,248,718 - DVU2149 0.69 -0.6 2,248,722 + DVU2149 0.69 -1.6 2,248,793 + DVU2149 0.73 +0.5 2,248,830 - DVU2149 0.75 -0.5 2,248,843 + DVU2149 0.76 -0.3 2,248,928 - DVU2149 0.81 -0.3 2,248,968 - DVU2149 0.83 -0.4 2,249,010 + DVU2149 0.86 -0.6 2,249,037 - DVU2149 0.87 -0.3 2,249,092 + -0.6 2,249,154 + +0.0 2,249,216 + +0.6 2,249,282 - +0.1 2,249,347 - dksA DVU2150 0.19 +1.7 2,249,454 + dksA DVU2150 0.49 -0.4 2,249,550 + dksA DVU2150 0.75 +1.0 2,249,558 - dksA DVU2150 0.77 +1.0 2,249,606 - +2.0 2,249,863 + -1.4 2,249,871 - -0.6 2,249,871 - -0.7 2,249,898 + -2.5 2,249,903 + -0.7 2,250,167 + +1.0 2,250,188 + -3.6 2,250,234 - -0.5
Or see this region's nucleotide sequence