Experiment: Pyruvate-Thiosulfate (60-15 mM) + 0.1% Yeast Extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt miaB and DORF8626 are separated by 132 nucleotides DORF8626 and DVU0987 are separated by 191 nucleotides DVU0987 and cbhK are separated by 457 nucleotides
DVU0984: miaB - tRNA-i(6)A37 modification enzyme MiaB (TIGR), at 1,080,262 to 1,081,611
miaB
DORF8626: DORF8626 - no description, at 1,081,744 to 1,081,989
DORF8626
DVU0987: DVU0987 - heavy metal-binding domain protein (TIGR), at 1,082,181 to 1,082,384
DVU0987
DVU0988: cbhK - carbohydrate kinase, PfkB family (TIGR), at 1,082,842 to 1,083,768
cbhK
Position (kb)
1082
1083 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1081.194 kb on - strand, within miaB at 1081.432 kb on + strand, within miaB at 1081.441 kb on - strand, within miaB at 1081.456 kb on + strand, within miaB at 1081.466 kb on + strand, within miaB at 1081.487 kb on + strand at 1081.487 kb on + strand at 1081.535 kb on - strand at 1081.598 kb on + strand at 1081.662 kb on - strand at 1081.669 kb on - strand at 1081.676 kb on + strand at 1081.800 kb on - strand, within DORF8626 at 1081.806 kb on - strand, within DORF8626 at 1081.859 kb on + strand, within DORF8626 at 1081.869 kb on + strand, within DORF8626 at 1081.869 kb on + strand, within DORF8626 at 1081.874 kb on + strand, within DORF8626 at 1081.877 kb on - strand, within DORF8626 at 1081.877 kb on - strand, within DORF8626 at 1081.877 kb on - strand, within DORF8626 at 1081.877 kb on - strand, within DORF8626 at 1081.879 kb on + strand, within DORF8626 at 1081.887 kb on - strand, within DORF8626 at 1081.944 kb on - strand, within DORF8626 at 1081.965 kb on - strand at 1082.016 kb on + strand at 1082.024 kb on - strand at 1082.042 kb on - strand at 1082.065 kb on - strand at 1082.115 kb on + strand at 1082.147 kb on + strand at 1082.196 kb on - strand at 1082.196 kb on - strand at 1082.243 kb on + strand, within DVU0987 at 1082.308 kb on + strand, within DVU0987 at 1082.316 kb on - strand, within DVU0987 at 1082.417 kb on - strand at 1082.541 kb on - strand at 1082.543 kb on + strand at 1082.551 kb on - strand at 1082.660 kb on - strand at 1082.681 kb on + strand at 1082.694 kb on - strand at 1082.758 kb on - strand at 1082.774 kb on - strand at 1082.784 kb on + strand at 1082.784 kb on + strand at 1082.866 kb on - strand at 1082.896 kb on - strand at 1082.904 kb on - strand at 1082.967 kb on + strand, within cbhK at 1082.967 kb on + strand, within cbhK at 1082.975 kb on - strand, within cbhK at 1082.975 kb on - strand, within cbhK at 1082.975 kb on - strand, within cbhK at 1083.046 kb on + strand, within cbhK at 1083.082 kb on + strand, within cbhK at 1083.196 kb on + strand, within cbhK
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Thiosulfate (60-15 mM) + 0.1% Yeast Extract remove 1,081,194 - miaB DVU0984 0.69 -0.3 1,081,432 + miaB DVU0984 0.87 -0.0 1,081,441 - miaB DVU0984 0.87 +0.1 1,081,456 + miaB DVU0984 0.88 -0.1 1,081,466 + miaB DVU0984 0.89 -0.2 1,081,487 + -4.1 1,081,487 + +0.1 1,081,535 - -0.2 1,081,598 + -0.6 1,081,662 - +1.2 1,081,669 - -0.6 1,081,676 + +0.2 1,081,800 - DORF8626 0.23 +1.0 1,081,806 - DORF8626 0.25 +1.0 1,081,859 + DORF8626 0.47 -0.5 1,081,869 + DORF8626 0.51 -0.3 1,081,869 + DORF8626 0.51 -0.1 1,081,874 + DORF8626 0.53 -0.0 1,081,877 - DORF8626 0.54 -1.4 1,081,877 - DORF8626 0.54 -0.3 1,081,877 - DORF8626 0.54 -0.5 1,081,877 - DORF8626 0.54 -0.4 1,081,879 + DORF8626 0.55 -0.3 1,081,887 - DORF8626 0.58 -0.8 1,081,944 - DORF8626 0.81 -0.8 1,081,965 - -0.7 1,082,016 + -0.1 1,082,024 - +0.6 1,082,042 - -0.2 1,082,065 - -1.1 1,082,115 + +0.7 1,082,147 + -0.1 1,082,196 - +2.5 1,082,196 - +0.2 1,082,243 + DVU0987 0.30 -0.0 1,082,308 + DVU0987 0.62 +0.8 1,082,316 - DVU0987 0.66 -0.0 1,082,417 - -1.0 1,082,541 - +0.5 1,082,543 + -0.0 1,082,551 - +2.0 1,082,660 - +1.0 1,082,681 + +0.5 1,082,694 - -0.1 1,082,758 - +0.2 1,082,774 - +1.4 1,082,784 + +2.3 1,082,784 + -0.0 1,082,866 - -0.3 1,082,896 - -1.0 1,082,904 - -0.2 1,082,967 + cbhK DVU0988 0.13 -0.1 1,082,967 + cbhK DVU0988 0.13 -0.6 1,082,975 - cbhK DVU0988 0.14 +2.5 1,082,975 - cbhK DVU0988 0.14 -0.1 1,082,975 - cbhK DVU0988 0.14 -0.0 1,083,046 + cbhK DVU0988 0.22 +0.1 1,083,082 + cbhK DVU0988 0.26 +1.0 1,083,196 + cbhK DVU0988 0.38 +0.1
Or see this region's nucleotide sequence