Experiment: L-aspartic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21455 and BWI76_RS21460 are separated by 271 nucleotides BWI76_RS21460 and BWI76_RS21465 are separated by 261 nucleotides BWI76_RS21465 and BWI76_RS21470 overlap by 4 nucleotides BWI76_RS21470 and BWI76_RS21475 are separated by 68 nucleotides
BWI76_RS21455: BWI76_RS21455 - outer membrane protein assembly factor BamD, at 4,494,985 to 4,495,722
_RS21455
BWI76_RS21460: BWI76_RS21460 - translation inhibitor protein RaiA, at 4,495,994 to 4,496,329
_RS21460
BWI76_RS21465: BWI76_RS21465 - bifunctional chorismate mutase/prephenate dehydratase, at 4,496,591 to 4,497,751
_RS21465
BWI76_RS21470: BWI76_RS21470 - gluconolaconase, at 4,497,748 to 4,498,620
_RS21470
BWI76_RS21475: BWI76_RS21475 - bifunctional chorismate mutase/prephenate dehydrogenase, at 4,498,689 to 4,499,810
_RS21475
Position (kb) 4496
4497
4498 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 4495.793 kb on - strand at 4495.796 kb on + strand at 4495.864 kb on + strand at 4495.864 kb on + strand at 4495.864 kb on + strand at 4495.871 kb on - strand at 4496.123 kb on - strand, within BWI76_RS21460 at 4496.414 kb on + strand at 4496.535 kb on - strand at 4496.535 kb on - strand at 4497.319 kb on + strand, within BWI76_RS21465 at 4497.341 kb on + strand, within BWI76_RS21465 at 4497.349 kb on - strand, within BWI76_RS21465 at 4497.354 kb on - strand, within BWI76_RS21465 at 4497.634 kb on - strand, within BWI76_RS21465 at 4497.644 kb on - strand at 4497.655 kb on + strand at 4497.709 kb on - strand at 4497.709 kb on - strand at 4497.888 kb on - strand, within BWI76_RS21470 at 4498.016 kb on + strand, within BWI76_RS21470 at 4498.440 kb on + strand, within BWI76_RS21470 at 4498.521 kb on + strand, within BWI76_RS21470 at 4498.563 kb on + strand at 4498.730 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-aspartic (N) remove 4,495,793 - -0.7 4,495,796 + +0.1 4,495,864 + +0.4 4,495,864 + -1.4 4,495,864 + +0.3 4,495,871 - -0.9 4,496,123 - BWI76_RS21460 0.38 +0.5 4,496,414 + +0.6 4,496,535 - -2.7 4,496,535 - -3.4 4,497,319 + BWI76_RS21465 0.63 -3.5 4,497,341 + BWI76_RS21465 0.65 -3.2 4,497,349 - BWI76_RS21465 0.65 -4.1 4,497,354 - BWI76_RS21465 0.66 -5.1 4,497,634 - BWI76_RS21465 0.90 -0.8 4,497,644 - -0.9 4,497,655 + -0.0 4,497,709 - -1.0 4,497,709 - +0.1 4,497,888 - BWI76_RS21470 0.16 -0.3 4,498,016 + BWI76_RS21470 0.31 -0.1 4,498,440 + BWI76_RS21470 0.79 +0.1 4,498,521 + BWI76_RS21470 0.89 +1.8 4,498,563 + +1.1 4,498,730 - -2.0
Or see this region's nucleotide sequence