Experiment: L-aspartic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20435 and BWI76_RS20440 are separated by 37 nucleotides BWI76_RS20440 and BWI76_RS20445 are separated by 167 nucleotides BWI76_RS20445 and BWI76_RS20450 are separated by 20 nucleotides
BWI76_RS20435: BWI76_RS20435 - chorismate synthase, at 4,277,326 to 4,278,411
_RS20435
BWI76_RS20440: BWI76_RS20440 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 4,278,449 to 4,279,381
_RS20440
BWI76_RS20445: BWI76_RS20445 - endonuclease SmrB, at 4,279,549 to 4,280,100
_RS20445
BWI76_RS20450: BWI76_RS20450 - phosphohistidine phosphatase, at 4,280,121 to 4,280,606
_RS20450
Position (kb) 4278
4279
4280 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4277.528 kb on + strand, within BWI76_RS20435 at 4278.248 kb on - strand, within BWI76_RS20435 at 4278.348 kb on - strand at 4278.588 kb on - strand, within BWI76_RS20440 at 4278.639 kb on + strand, within BWI76_RS20440 at 4278.867 kb on + strand, within BWI76_RS20440 at 4278.867 kb on + strand, within BWI76_RS20440 at 4279.395 kb on - strand at 4279.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-aspartic (N) remove 4,277,528 + BWI76_RS20435 0.19 -0.5 4,278,248 - BWI76_RS20435 0.85 -2.9 4,278,348 - -5.2 4,278,588 - BWI76_RS20440 0.15 +0.2 4,278,639 + BWI76_RS20440 0.20 -2.0 4,278,867 + BWI76_RS20440 0.45 -1.7 4,278,867 + BWI76_RS20440 0.45 -2.4 4,279,395 - -0.1 4,279,396 - -1.0
Or see this region's nucleotide sequence