Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08525

Experiment: L-aspartic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08515 and BWI76_RS08520 are separated by 5 nucleotidesBWI76_RS08520 and BWI76_RS08525 are separated by 3 nucleotidesBWI76_RS08525 and BWI76_RS08530 are separated by 105 nucleotides BWI76_RS08515: BWI76_RS08515 - acyl-CoA thioesterase YbgC, at 1,803,387 to 1,803,791 _RS08515 BWI76_RS08520: BWI76_RS08520 - colicin transporter TolQ, at 1,803,797 to 1,804,480 _RS08520 BWI76_RS08525: BWI76_RS08525 - colicin transporter, at 1,804,484 to 1,804,912 _RS08525 BWI76_RS08530: BWI76_RS08530 - protein TolA, at 1,805,018 to 1,806,355 _RS08530 Position (kb) 1804 1805Strain fitness (log2 ratio) -2 -1 0 1at 1803.764 kb on + strandat 1803.812 kb on - strandat 1803.823 kb on + strandat 1804.000 kb on - strand, within BWI76_RS08520at 1804.358 kb on + strand, within BWI76_RS08520at 1804.368 kb on + strand, within BWI76_RS08520at 1804.421 kb on - strandat 1804.433 kb on + strandat 1804.486 kb on + strandat 1804.491 kb on + strandat 1804.546 kb on + strand, within BWI76_RS08525at 1804.697 kb on + strand, within BWI76_RS08525at 1804.753 kb on + strand, within BWI76_RS08525at 1805.247 kb on + strand, within BWI76_RS08530

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Per-strain Table

Position Strand Gene LocusTag Fraction L-aspartic (N)
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1,803,764 + -0.1
1,803,812 - +0.2
1,803,823 + -1.5
1,804,000 - BWI76_RS08520 0.30 -1.3
1,804,358 + BWI76_RS08520 0.82 -0.8
1,804,368 + BWI76_RS08520 0.83 -2.1
1,804,421 - -1.4
1,804,433 + -0.3
1,804,486 + -2.3
1,804,491 + -1.4
1,804,546 + BWI76_RS08525 0.14 -2.6
1,804,697 + BWI76_RS08525 0.50 -2.1
1,804,753 + BWI76_RS08525 0.63 -0.2
1,805,247 + BWI76_RS08530 0.17 -0.6

Or see this region's nucleotide sequence