Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06120

Experiment: L-aspartic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06110 and BWI76_RS06115 overlap by 4 nucleotidesBWI76_RS06115 and BWI76_RS06120 are separated by 255 nucleotidesBWI76_RS06120 and BWI76_RS06125 are separated by 151 nucleotides BWI76_RS06110: BWI76_RS06110 - hypothetical protein, at 1,284,849 to 1,285,955 _RS06110 BWI76_RS06115: BWI76_RS06115 - membrane protein, at 1,285,952 to 1,286,254 _RS06115 BWI76_RS06120: BWI76_RS06120 - membrane protein, at 1,286,510 to 1,286,713 _RS06120 BWI76_RS06125: BWI76_RS06125 - D-alanine--D-alanine ligase A, at 1,286,865 to 1,287,962 _RS06125 Position (kb) 1286 1287Strain fitness (log2 ratio) -2 -1 0 1 2at 1285.944 kb on - strandat 1286.548 kb on - strand, within BWI76_RS06120at 1286.553 kb on - strand, within BWI76_RS06120at 1286.766 kb on + strandat 1286.965 kb on + strandat 1286.972 kb on + strandat 1287.054 kb on - strand, within BWI76_RS06125at 1287.253 kb on + strand, within BWI76_RS06125at 1287.364 kb on + strand, within BWI76_RS06125at 1287.477 kb on + strand, within BWI76_RS06125at 1287.490 kb on - strand, within BWI76_RS06125at 1287.506 kb on + strand, within BWI76_RS06125at 1287.657 kb on + strand, within BWI76_RS06125at 1287.705 kb on + strand, within BWI76_RS06125

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Per-strain Table

Position Strand Gene LocusTag Fraction L-aspartic (N)
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1,285,944 - -0.6
1,286,548 - BWI76_RS06120 0.19 -1.4
1,286,553 - BWI76_RS06120 0.21 +0.0
1,286,766 + +0.3
1,286,965 + +0.4
1,286,972 + -2.7
1,287,054 - BWI76_RS06125 0.17 -0.1
1,287,253 + BWI76_RS06125 0.35 +1.9
1,287,364 + BWI76_RS06125 0.45 -0.0
1,287,477 + BWI76_RS06125 0.56 +1.6
1,287,490 - BWI76_RS06125 0.57 +0.1
1,287,506 + BWI76_RS06125 0.58 +0.3
1,287,657 + BWI76_RS06125 0.72 +0.6
1,287,705 + BWI76_RS06125 0.77 +1.2

Or see this region's nucleotide sequence