Experiment: L-aspartic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05825 and BWI76_RS05830 are separated by 163 nucleotides BWI76_RS05830 and BWI76_RS05835 are separated by 112 nucleotides BWI76_RS05835 and BWI76_RS05840 are separated by 10 nucleotides
BWI76_RS05825: BWI76_RS05825 - putative site specific integrase, at 1,223,637 to 1,224,821
_RS05825
BWI76_RS05830: BWI76_RS05830 - tRNA-Thr, at 1,224,985 to 1,225,060
_RS05830
BWI76_RS05835: BWI76_RS05835 - gamma-glutamyl-phosphate reductase, at 1,225,173 to 1,226,426
_RS05835
BWI76_RS05840: BWI76_RS05840 - glutamate 5-kinase, at 1,226,437 to 1,227,540
_RS05840
Position (kb)
1225
1226
1227 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1224.182 kb on - strand, within BWI76_RS05825 at 1224.224 kb on + strand, within BWI76_RS05825 at 1224.249 kb on - strand, within BWI76_RS05825 at 1224.302 kb on + strand, within BWI76_RS05825 at 1224.302 kb on + strand, within BWI76_RS05825 at 1224.310 kb on - strand, within BWI76_RS05825 at 1224.310 kb on - strand, within BWI76_RS05825 at 1224.310 kb on - strand, within BWI76_RS05825 at 1224.310 kb on - strand, within BWI76_RS05825 at 1224.315 kb on - strand, within BWI76_RS05825 at 1224.323 kb on - strand, within BWI76_RS05825 at 1224.370 kb on + strand, within BWI76_RS05825 at 1224.378 kb on - strand, within BWI76_RS05825 at 1224.378 kb on - strand, within BWI76_RS05825 at 1224.508 kb on + strand, within BWI76_RS05825 at 1224.722 kb on + strand at 1224.765 kb on - strand at 1224.766 kb on + strand at 1224.772 kb on - strand at 1224.869 kb on - strand at 1225.339 kb on + strand, within BWI76_RS05835 at 1225.528 kb on - strand, within BWI76_RS05835 at 1226.142 kb on + strand, within BWI76_RS05835 at 1226.142 kb on + strand, within BWI76_RS05835 at 1226.310 kb on + strand at 1226.318 kb on + strand at 1226.364 kb on + strand at 1226.501 kb on - strand at 1226.919 kb on - strand, within BWI76_RS05840 at 1226.955 kb on + strand, within BWI76_RS05840 at 1226.980 kb on - strand, within BWI76_RS05840 at 1227.056 kb on - strand, within BWI76_RS05840 at 1227.180 kb on + strand, within BWI76_RS05840 at 1227.356 kb on - strand, within BWI76_RS05840 at 1227.387 kb on + strand, within BWI76_RS05840
Per-strain Table
Position Strand Gene LocusTag Fraction L-aspartic (N) remove 1,224,182 - BWI76_RS05825 0.46 -2.4 1,224,224 + BWI76_RS05825 0.50 +0.6 1,224,249 - BWI76_RS05825 0.52 +0.3 1,224,302 + BWI76_RS05825 0.56 -0.3 1,224,302 + BWI76_RS05825 0.56 +0.3 1,224,310 - BWI76_RS05825 0.57 -2.2 1,224,310 - BWI76_RS05825 0.57 -1.2 1,224,310 - BWI76_RS05825 0.57 +0.7 1,224,310 - BWI76_RS05825 0.57 +1.2 1,224,315 - BWI76_RS05825 0.57 +0.3 1,224,323 - BWI76_RS05825 0.58 +0.5 1,224,370 + BWI76_RS05825 0.62 +0.2 1,224,378 - BWI76_RS05825 0.63 -0.6 1,224,378 - BWI76_RS05825 0.63 +1.7 1,224,508 + BWI76_RS05825 0.74 +0.3 1,224,722 + +0.2 1,224,765 - +0.5 1,224,766 + +2.1 1,224,772 - -0.6 1,224,869 - -0.3 1,225,339 + BWI76_RS05835 0.13 -4.1 1,225,528 - BWI76_RS05835 0.28 -4.1 1,226,142 + BWI76_RS05835 0.77 -3.5 1,226,142 + BWI76_RS05835 0.77 -2.2 1,226,310 + -4.1 1,226,318 + -3.9 1,226,364 + -0.6 1,226,501 - -2.1 1,226,919 - BWI76_RS05840 0.44 -2.0 1,226,955 + BWI76_RS05840 0.47 -3.3 1,226,980 - BWI76_RS05840 0.49 -5.5 1,227,056 - BWI76_RS05840 0.56 -1.0 1,227,180 + BWI76_RS05840 0.67 -2.9 1,227,356 - BWI76_RS05840 0.83 -2.6 1,227,387 + BWI76_RS05840 0.86 -2.3
Or see this region's nucleotide sequence