Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05835

Experiment: L-aspartic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05825 and BWI76_RS05830 are separated by 163 nucleotidesBWI76_RS05830 and BWI76_RS05835 are separated by 112 nucleotidesBWI76_RS05835 and BWI76_RS05840 are separated by 10 nucleotides BWI76_RS05825: BWI76_RS05825 - putative site specific integrase, at 1,223,637 to 1,224,821 _RS05825 BWI76_RS05830: BWI76_RS05830 - tRNA-Thr, at 1,224,985 to 1,225,060 _RS05830 BWI76_RS05835: BWI76_RS05835 - gamma-glutamyl-phosphate reductase, at 1,225,173 to 1,226,426 _RS05835 BWI76_RS05840: BWI76_RS05840 - glutamate 5-kinase, at 1,226,437 to 1,227,540 _RS05840 Position (kb) 1225 1226 1227Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1224.182 kb on - strand, within BWI76_RS05825at 1224.224 kb on + strand, within BWI76_RS05825at 1224.249 kb on - strand, within BWI76_RS05825at 1224.302 kb on + strand, within BWI76_RS05825at 1224.302 kb on + strand, within BWI76_RS05825at 1224.310 kb on - strand, within BWI76_RS05825at 1224.310 kb on - strand, within BWI76_RS05825at 1224.310 kb on - strand, within BWI76_RS05825at 1224.310 kb on - strand, within BWI76_RS05825at 1224.315 kb on - strand, within BWI76_RS05825at 1224.323 kb on - strand, within BWI76_RS05825at 1224.370 kb on + strand, within BWI76_RS05825at 1224.378 kb on - strand, within BWI76_RS05825at 1224.378 kb on - strand, within BWI76_RS05825at 1224.508 kb on + strand, within BWI76_RS05825at 1224.722 kb on + strandat 1224.765 kb on - strandat 1224.766 kb on + strandat 1224.772 kb on - strandat 1224.869 kb on - strandat 1225.339 kb on + strand, within BWI76_RS05835at 1225.528 kb on - strand, within BWI76_RS05835at 1226.142 kb on + strand, within BWI76_RS05835at 1226.142 kb on + strand, within BWI76_RS05835at 1226.310 kb on + strandat 1226.318 kb on + strandat 1226.364 kb on + strandat 1226.501 kb on - strandat 1226.919 kb on - strand, within BWI76_RS05840at 1226.955 kb on + strand, within BWI76_RS05840at 1226.980 kb on - strand, within BWI76_RS05840at 1227.056 kb on - strand, within BWI76_RS05840at 1227.180 kb on + strand, within BWI76_RS05840at 1227.356 kb on - strand, within BWI76_RS05840at 1227.387 kb on + strand, within BWI76_RS05840

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Per-strain Table

Position Strand Gene LocusTag Fraction L-aspartic (N)
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1,224,182 - BWI76_RS05825 0.46 -2.4
1,224,224 + BWI76_RS05825 0.50 +0.6
1,224,249 - BWI76_RS05825 0.52 +0.3
1,224,302 + BWI76_RS05825 0.56 -0.3
1,224,302 + BWI76_RS05825 0.56 +0.3
1,224,310 - BWI76_RS05825 0.57 -2.2
1,224,310 - BWI76_RS05825 0.57 -1.2
1,224,310 - BWI76_RS05825 0.57 +0.7
1,224,310 - BWI76_RS05825 0.57 +1.2
1,224,315 - BWI76_RS05825 0.57 +0.3
1,224,323 - BWI76_RS05825 0.58 +0.5
1,224,370 + BWI76_RS05825 0.62 +0.2
1,224,378 - BWI76_RS05825 0.63 -0.6
1,224,378 - BWI76_RS05825 0.63 +1.7
1,224,508 + BWI76_RS05825 0.74 +0.3
1,224,722 + +0.2
1,224,765 - +0.5
1,224,766 + +2.1
1,224,772 - -0.6
1,224,869 - -0.3
1,225,339 + BWI76_RS05835 0.13 -4.1
1,225,528 - BWI76_RS05835 0.28 -4.1
1,226,142 + BWI76_RS05835 0.77 -3.5
1,226,142 + BWI76_RS05835 0.77 -2.2
1,226,310 + -4.1
1,226,318 + -3.9
1,226,364 + -0.6
1,226,501 - -2.1
1,226,919 - BWI76_RS05840 0.44 -2.0
1,226,955 + BWI76_RS05840 0.47 -3.3
1,226,980 - BWI76_RS05840 0.49 -5.5
1,227,056 - BWI76_RS05840 0.56 -1.0
1,227,180 + BWI76_RS05840 0.67 -2.9
1,227,356 - BWI76_RS05840 0.83 -2.6
1,227,387 + BWI76_RS05840 0.86 -2.3

Or see this region's nucleotide sequence