Experiment: L-aspartic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04515 and BWI76_RS04520 are separated by 2 nucleotides BWI76_RS04520 and BWI76_RS04525 overlap by 4 nucleotides BWI76_RS04525 and BWI76_RS04530 are separated by 112 nucleotides
BWI76_RS04515: BWI76_RS04515 - Co2+/Mg2+ efflux protein ApaG, at 934,244 to 934,621
_RS04515
BWI76_RS04520: BWI76_RS04520 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase, at 934,624 to 935,445
_RS04520
BWI76_RS04525: BWI76_RS04525 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 935,442 to 936,431
_RS04525
BWI76_RS04530: BWI76_RS04530 - peptidylprolyl isomerase SurA, at 936,544 to 937,830
_RS04530
Position (kb) 935
936
937 Strain fitness (log2 ratio) -1
0
1
2 at 934.605 kb on - strand at 935.290 kb on + strand, within BWI76_RS04520 at 935.422 kb on + strand at 935.511 kb on + strand at 935.533 kb on - strand at 935.946 kb on - strand, within BWI76_RS04525 at 935.951 kb on - strand, within BWI76_RS04525 at 935.997 kb on - strand, within BWI76_RS04525 at 936.736 kb on + strand, within BWI76_RS04530 at 936.830 kb on - strand, within BWI76_RS04530 at 936.904 kb on - strand, within BWI76_RS04530 at 937.353 kb on + strand, within BWI76_RS04530
Per-strain Table
Position Strand Gene LocusTag Fraction L-aspartic (N) remove 934,605 - -0.2 935,290 + BWI76_RS04520 0.81 +0.2 935,422 + +0.1 935,511 + -1.4 935,533 - -0.7 935,946 - BWI76_RS04525 0.51 -1.4 935,951 - BWI76_RS04525 0.51 +0.2 935,997 - BWI76_RS04525 0.56 -1.5 936,736 + BWI76_RS04530 0.15 +0.8 936,830 - BWI76_RS04530 0.22 -0.6 936,904 - BWI76_RS04530 0.28 -0.1 937,353 + BWI76_RS04530 0.63 +2.1
Or see this region's nucleotide sequence