Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04465

Experiment: L-aspartic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04460 and BWI76_RS04465 are separated by 457 nucleotidesBWI76_RS04465 and BWI76_RS04470 are separated by 18 nucleotides BWI76_RS04460: BWI76_RS04460 - 4-hydroxy-tetrahydrodipicolinate reductase, at 921,267 to 922,088 _RS04460 BWI76_RS04465: BWI76_RS04465 - carbamoyl-phosphate synthase small subunit, at 922,546 to 923,694 _RS04465 BWI76_RS04470: BWI76_RS04470 - carbamoyl phosphate synthase large subunit, at 923,713 to 926,937 _RS04470 Position (kb) 922 923 924Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 922.434 kb on + strandat 922.440 kb on - strandat 922.440 kb on - strandat 922.599 kb on + strandat 922.828 kb on + strand, within BWI76_RS04465at 922.836 kb on + strand, within BWI76_RS04465at 922.836 kb on - strand, within BWI76_RS04465at 922.897 kb on - strand, within BWI76_RS04465at 923.052 kb on - strand, within BWI76_RS04465at 923.206 kb on + strand, within BWI76_RS04465at 923.623 kb on - strandat 923.628 kb on - strandat 923.794 kb on - strandat 923.808 kb on + strandat 923.816 kb on - strandat 923.855 kb on + strandat 923.862 kb on + strandat 923.879 kb on - strandat 923.995 kb on + strandat 923.995 kb on + strandat 924.003 kb on - strandat 924.256 kb on + strand, within BWI76_RS04470at 924.264 kb on - strandat 924.575 kb on - strand, within BWI76_RS04470

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Per-strain Table

Position Strand Gene LocusTag Fraction L-aspartic (N)
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922,434 + +0.1
922,440 - -0.6
922,440 - +0.9
922,599 + -3.2
922,828 + BWI76_RS04465 0.25 -4.9
922,836 + BWI76_RS04465 0.25 -5.7
922,836 - BWI76_RS04465 0.25 -4.0
922,897 - BWI76_RS04465 0.31 -1.2
923,052 - BWI76_RS04465 0.44 -4.0
923,206 + BWI76_RS04465 0.57 -2.6
923,623 - -2.9
923,628 - -4.7
923,794 - -5.3
923,808 + -3.2
923,816 - -1.1
923,855 + -2.4
923,862 + -5.2
923,879 - -5.0
923,995 + -2.4
923,995 + -1.1
924,003 - -3.0
924,256 + BWI76_RS04470 0.17 -2.2
924,264 - -0.7
924,575 - BWI76_RS04470 0.27 -4.3

Or see this region's nucleotide sequence