Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01615

Experiment: L-aspartic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01610 and BWI76_RS01615 are separated by 15 nucleotidesBWI76_RS01615 and BWI76_RS01620 are separated by 4 nucleotidesBWI76_RS01620 and BWI76_RS01625 are separated by 142 nucleotidesBWI76_RS01625 and BWI76_RS01630 are separated by 53 nucleotides BWI76_RS01610: BWI76_RS01610 - phosphoribosylamine--glycine ligase, at 326,521 to 327,813 _RS01610 BWI76_RS01615: BWI76_RS01615 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/inosine monophosphate cyclohydrolase, at 327,829 to 329,418 _RS01615 BWI76_RS01620: BWI76_RS01620 - hypothetical protein, at 329,423 to 329,650 _RS01620 BWI76_RS01625: BWI76_RS01625 - hypothetical protein, at 329,793 to 329,998 _RS01625 BWI76_RS01630: BWI76_RS01630 - 16S ribosomal RNA, at 330,052 to 331,605 _RS01630 Position (kb) 327 328 329 330Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 326.977 kb on - strand, within BWI76_RS01610at 327.022 kb on - strand, within BWI76_RS01610at 327.081 kb on + strand, within BWI76_RS01610at 327.113 kb on - strand, within BWI76_RS01610at 327.206 kb on + strand, within BWI76_RS01610at 327.516 kb on + strand, within BWI76_RS01610at 327.586 kb on - strand, within BWI76_RS01610at 327.628 kb on + strand, within BWI76_RS01610at 327.648 kb on - strand, within BWI76_RS01610at 327.712 kb on - strandat 327.862 kb on - strandat 328.002 kb on + strand, within BWI76_RS01615at 328.075 kb on + strand, within BWI76_RS01615at 328.100 kb on + strand, within BWI76_RS01615at 328.127 kb on + strand, within BWI76_RS01615at 328.135 kb on - strand, within BWI76_RS01615at 328.249 kb on + strand, within BWI76_RS01615at 328.268 kb on + strand, within BWI76_RS01615at 328.357 kb on + strand, within BWI76_RS01615at 328.543 kb on + strand, within BWI76_RS01615at 328.608 kb on - strand, within BWI76_RS01615at 328.639 kb on - strand, within BWI76_RS01615at 328.689 kb on - strand, within BWI76_RS01615at 328.739 kb on + strand, within BWI76_RS01615at 328.775 kb on - strand, within BWI76_RS01615at 328.858 kb on + strand, within BWI76_RS01615at 328.896 kb on + strand, within BWI76_RS01615at 328.999 kb on - strand, within BWI76_RS01615at 329.130 kb on + strand, within BWI76_RS01615at 329.218 kb on - strand, within BWI76_RS01615at 329.306 kb on + strandat 329.312 kb on - strandat 329.368 kb on - strandat 329.428 kb on - strandat 329.594 kb on + strand, within BWI76_RS01620at 329.594 kb on + strand, within BWI76_RS01620at 329.600 kb on + strand, within BWI76_RS01620at 329.605 kb on + strand, within BWI76_RS01620

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Per-strain Table

Position Strand Gene LocusTag Fraction L-aspartic (N)
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326,977 - BWI76_RS01610 0.35 -2.9
327,022 - BWI76_RS01610 0.39 -4.0
327,081 + BWI76_RS01610 0.43 -5.4
327,113 - BWI76_RS01610 0.46 -2.1
327,206 + BWI76_RS01610 0.53 -5.0
327,516 + BWI76_RS01610 0.77 -2.8
327,586 - BWI76_RS01610 0.82 -4.6
327,628 + BWI76_RS01610 0.86 -2.4
327,648 - BWI76_RS01610 0.87 -5.4
327,712 - -3.8
327,862 - -4.4
328,002 + BWI76_RS01615 0.11 -3.5
328,075 + BWI76_RS01615 0.15 -2.8
328,100 + BWI76_RS01615 0.17 +0.6
328,127 + BWI76_RS01615 0.19 -4.9
328,135 - BWI76_RS01615 0.19 -6.5
328,249 + BWI76_RS01615 0.26 -4.9
328,268 + BWI76_RS01615 0.28 -4.6
328,357 + BWI76_RS01615 0.33 -5.2
328,543 + BWI76_RS01615 0.45 -2.9
328,608 - BWI76_RS01615 0.49 -3.2
328,639 - BWI76_RS01615 0.51 -3.1
328,689 - BWI76_RS01615 0.54 -2.3
328,739 + BWI76_RS01615 0.57 -3.0
328,775 - BWI76_RS01615 0.59 -0.8
328,858 + BWI76_RS01615 0.65 -4.0
328,896 + BWI76_RS01615 0.67 -2.0
328,999 - BWI76_RS01615 0.74 -1.7
329,130 + BWI76_RS01615 0.82 -4.9
329,218 - BWI76_RS01615 0.87 -5.8
329,306 + -1.2
329,312 - -2.2
329,368 - -3.6
329,428 - -2.4
329,594 + BWI76_RS01620 0.75 -0.2
329,594 + BWI76_RS01620 0.75 +0.1
329,600 + BWI76_RS01620 0.78 -0.7
329,605 + BWI76_RS01620 0.80 -0.5

Or see this region's nucleotide sequence