Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00980

Experiment: L-aspartic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00975 and BWI76_RS00980 are separated by 2 nucleotidesBWI76_RS00980 and BWI76_RS00985 are separated by 58 nucleotides BWI76_RS00975: BWI76_RS00975 - dihydroxy-acid dehydratase, at 198,294 to 200,144 _RS00975 BWI76_RS00980: BWI76_RS00980 - PLP-dependent threonine dehydratase, at 200,147 to 201,691 _RS00980 BWI76_RS00985: BWI76_RS00985 - transcriptional regulator IlvY, at 201,750 to 202,640 _RS00985 Position (kb) 200 201 202Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 199.226 kb on + strand, within BWI76_RS00975at 199.226 kb on + strand, within BWI76_RS00975at 199.226 kb on + strand, within BWI76_RS00975at 199.226 kb on + strand, within BWI76_RS00975at 199.234 kb on - strand, within BWI76_RS00975at 199.234 kb on - strand, within BWI76_RS00975at 199.740 kb on - strand, within BWI76_RS00975at 199.806 kb on + strand, within BWI76_RS00975at 199.877 kb on - strand, within BWI76_RS00975at 199.960 kb on - strandat 200.009 kb on + strandat 200.062 kb on + strandat 200.070 kb on - strandat 200.192 kb on - strandat 200.207 kb on - strandat 200.207 kb on - strandat 200.229 kb on - strandat 200.244 kb on - strandat 200.325 kb on + strand, within BWI76_RS00980at 200.325 kb on + strand, within BWI76_RS00980at 200.325 kb on + strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.333 kb on - strand, within BWI76_RS00980at 200.558 kb on + strand, within BWI76_RS00980at 200.652 kb on - strand, within BWI76_RS00980at 200.792 kb on + strand, within BWI76_RS00980at 200.845 kb on - strand, within BWI76_RS00980at 200.845 kb on - strand, within BWI76_RS00980at 200.869 kb on - strand, within BWI76_RS00980at 200.929 kb on + strand, within BWI76_RS00980at 201.380 kb on - strand, within BWI76_RS00980at 201.454 kb on - strandat 201.505 kb on + strand, within BWI76_RS00980at 201.565 kb on - strandat 201.629 kb on - strandat 201.918 kb on - strand, within BWI76_RS00985at 201.928 kb on - strand, within BWI76_RS00985at 201.974 kb on + strand, within BWI76_RS00985at 202.030 kb on - strand, within BWI76_RS00985at 202.043 kb on - strand, within BWI76_RS00985at 202.101 kb on - strand, within BWI76_RS00985at 202.114 kb on + strand, within BWI76_RS00985at 202.114 kb on + strand, within BWI76_RS00985at 202.177 kb on + strand, within BWI76_RS00985at 202.264 kb on + strand, within BWI76_RS00985at 202.444 kb on + strand, within BWI76_RS00985at 202.515 kb on + strand, within BWI76_RS00985at 202.560 kb on + strandat 202.610 kb on + strandat 202.625 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-aspartic (N)
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199,226 + BWI76_RS00975 0.50 -4.9
199,226 + BWI76_RS00975 0.50 -0.6
199,226 + BWI76_RS00975 0.50 -6.6
199,226 + BWI76_RS00975 0.50 -0.9
199,234 - BWI76_RS00975 0.51 -2.7
199,234 - BWI76_RS00975 0.51 -4.9
199,740 - BWI76_RS00975 0.78 -0.7
199,806 + BWI76_RS00975 0.82 -1.7
199,877 - BWI76_RS00975 0.86 -4.6
199,960 - -3.0
200,009 + -3.0
200,062 + -0.7
200,070 - -3.6
200,192 - -2.5
200,207 - -4.1
200,207 - -3.6
200,229 - -2.6
200,244 - -3.8
200,325 + BWI76_RS00980 0.12 -3.6
200,325 + BWI76_RS00980 0.12 -2.6
200,325 + BWI76_RS00980 0.12 -3.8
200,333 - BWI76_RS00980 0.12 -3.2
200,333 - BWI76_RS00980 0.12 -3.3
200,333 - BWI76_RS00980 0.12 -4.5
200,333 - BWI76_RS00980 0.12 -3.3
200,333 - BWI76_RS00980 0.12 -4.2
200,333 - BWI76_RS00980 0.12 -2.5
200,333 - BWI76_RS00980 0.12 -2.2
200,333 - BWI76_RS00980 0.12 -3.2
200,333 - BWI76_RS00980 0.12 -4.6
200,333 - BWI76_RS00980 0.12 -3.1
200,558 + BWI76_RS00980 0.27 -5.5
200,652 - BWI76_RS00980 0.33 -2.1
200,792 + BWI76_RS00980 0.42 -3.2
200,845 - BWI76_RS00980 0.45 +0.7
200,845 - BWI76_RS00980 0.45 -4.3
200,869 - BWI76_RS00980 0.47 -4.4
200,929 + BWI76_RS00980 0.51 -4.8
201,380 - BWI76_RS00980 0.80 -1.3
201,454 - +0.3
201,505 + BWI76_RS00980 0.88 -3.6
201,565 - -1.6
201,629 - -2.0
201,918 - BWI76_RS00985 0.19 -1.7
201,928 - BWI76_RS00985 0.20 -3.3
201,974 + BWI76_RS00985 0.25 +0.6
202,030 - BWI76_RS00985 0.31 -2.4
202,043 - BWI76_RS00985 0.33 -0.6
202,101 - BWI76_RS00985 0.39 -1.3
202,114 + BWI76_RS00985 0.41 -0.4
202,114 + BWI76_RS00985 0.41 -2.3
202,177 + BWI76_RS00985 0.48 -2.4
202,264 + BWI76_RS00985 0.58 -2.3
202,444 + BWI76_RS00985 0.78 +0.3
202,515 + BWI76_RS00985 0.86 -0.4
202,560 + -1.3
202,610 + +1.1
202,625 - -2.5

Or see this region's nucleotide sequence