Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20480 and BBR_RS21170 overlap by 4 nucleotides BBR_RS21170 and BBR_RS20485 are separated by 3 nucleotides BBR_RS20485 and BBR_RS20490 are separated by 31 nucleotides
BBR_RS20480: BBR_RS20480 - membrane protein, at 2,420,522 to 2,421,532
_RS20480
BBR_RS21170: BBR_RS21170 - membrane protein insertion efficiency factor YidD, at 2,421,529 to 2,421,846
_RS21170
BBR_RS20485: BBR_RS20485 - ribonuclease P protein component, at 2,421,850 to 2,422,200
_RS20485
BBR_RS20490: BBR_RS20490 - 50S ribosomal protein L34, at 2,422,232 to 2,422,366
_RS20490
Position (kb)
2421
2422 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2420.600 kb on - strand at 2420.634 kb on - strand, within BBR_RS20480 at 2421.470 kb on - strand at 2421.482 kb on - strand at 2421.497 kb on - strand at 2421.521 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.572 kb on - strand, within BBR_RS21170 at 2421.577 kb on - strand, within BBR_RS21170 at 2421.577 kb on - strand, within BBR_RS21170 at 2421.585 kb on - strand, within BBR_RS21170 at 2421.587 kb on - strand, within BBR_RS21170 at 2421.587 kb on - strand, within BBR_RS21170 at 2421.587 kb on - strand, within BBR_RS21170 at 2421.587 kb on - strand, within BBR_RS21170 at 2421.595 kb on - strand, within BBR_RS21170 at 2421.595 kb on - strand, within BBR_RS21170 at 2421.630 kb on - strand, within BBR_RS21170 at 2421.644 kb on - strand, within BBR_RS21170 at 2421.644 kb on - strand, within BBR_RS21170 at 2421.660 kb on - strand, within BBR_RS21170 at 2421.660 kb on - strand, within BBR_RS21170 at 2421.660 kb on - strand, within BBR_RS21170 at 2421.660 kb on - strand, within BBR_RS21170 at 2421.693 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.715 kb on - strand, within BBR_RS21170 at 2421.715 kb on - strand, within BBR_RS21170 at 2421.715 kb on - strand, within BBR_RS21170 at 2421.715 kb on - strand, within BBR_RS21170 at 2421.746 kb on - strand, within BBR_RS21170 at 2421.760 kb on - strand, within BBR_RS21170 at 2421.767 kb on - strand, within BBR_RS21170 at 2421.796 kb on - strand, within BBR_RS21170 at 2421.806 kb on - strand, within BBR_RS21170 at 2421.807 kb on - strand, within BBR_RS21170 at 2421.813 kb on - strand, within BBR_RS21170 at 2421.825 kb on - strand at 2421.835 kb on - strand at 2421.835 kb on - strand at 2421.840 kb on - strand at 2421.840 kb on - strand at 2421.840 kb on - strand at 2421.844 kb on - strand at 2421.845 kb on - strand at 2422.192 kb on - strand at 2422.192 kb on - strand at 2422.201 kb on - strand at 2422.209 kb on + strand at 2422.244 kb on + strand at 2422.443 kb on + strand at 2422.443 kb on + strand at 2422.490 kb on + strand at 2422.522 kb on - strand at 2422.523 kb on + strand at 2422.523 kb on + strand at 2422.544 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 2,420,600 - -0.1 2,420,634 - BBR_RS20480 0.11 -1.0 2,421,470 - -2.1 2,421,482 - -1.9 2,421,497 - -1.0 2,421,521 - -1.4 2,421,551 - -0.3 2,421,551 - -1.0 2,421,551 - -1.2 2,421,551 - -1.9 2,421,551 - -0.3 2,421,551 - -1.0 2,421,551 - -0.5 2,421,551 - -1.4 2,421,551 - -1.2 2,421,551 - -0.3 2,421,551 - -1.2 2,421,551 - -0.5 2,421,572 - BBR_RS21170 0.14 -1.1 2,421,577 - BBR_RS21170 0.15 +0.3 2,421,577 - BBR_RS21170 0.15 -0.8 2,421,585 - BBR_RS21170 0.18 -2.0 2,421,587 - BBR_RS21170 0.18 -3.0 2,421,587 - BBR_RS21170 0.18 -0.4 2,421,587 - BBR_RS21170 0.18 -1.7 2,421,587 - BBR_RS21170 0.18 -2.1 2,421,595 - BBR_RS21170 0.21 -1.0 2,421,595 - BBR_RS21170 0.21 -1.9 2,421,630 - BBR_RS21170 0.32 -2.0 2,421,644 - BBR_RS21170 0.36 -1.6 2,421,644 - BBR_RS21170 0.36 -1.2 2,421,660 - BBR_RS21170 0.41 -1.3 2,421,660 - BBR_RS21170 0.41 -3.2 2,421,660 - BBR_RS21170 0.41 -0.3 2,421,660 - BBR_RS21170 0.41 -1.5 2,421,693 - BBR_RS21170 0.52 -2.0 2,421,699 - BBR_RS21170 0.53 +0.4 2,421,699 - BBR_RS21170 0.53 +0.1 2,421,699 - BBR_RS21170 0.53 -2.1 2,421,699 - BBR_RS21170 0.53 -1.9 2,421,699 - BBR_RS21170 0.53 -2.0 2,421,715 - BBR_RS21170 0.58 -1.5 2,421,715 - BBR_RS21170 0.58 -1.9 2,421,715 - BBR_RS21170 0.58 -0.2 2,421,715 - BBR_RS21170 0.58 -1.7 2,421,746 - BBR_RS21170 0.68 -1.0 2,421,760 - BBR_RS21170 0.73 -1.8 2,421,767 - BBR_RS21170 0.75 -2.5 2,421,796 - BBR_RS21170 0.84 -0.8 2,421,806 - BBR_RS21170 0.87 -2.2 2,421,807 - BBR_RS21170 0.87 -1.2 2,421,813 - BBR_RS21170 0.89 -1.4 2,421,825 - -1.7 2,421,835 - -2.5 2,421,835 - -2.1 2,421,840 - -2.6 2,421,840 - -2.1 2,421,840 - -2.6 2,421,844 - -1.3 2,421,845 - -0.8 2,422,192 - -1.9 2,422,192 - -1.5 2,422,201 - -1.1 2,422,209 + +0.9 2,422,244 + -0.1 2,422,443 + -2.4 2,422,443 + -0.7 2,422,490 + +0.4 2,422,522 - +0.4 2,422,523 + +0.1 2,422,523 + +0.3 2,422,544 + -0.0
Or see this region's nucleotide sequence