Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15690 and BBR_RS20920 are separated by 154 nucleotides BBR_RS20920 and BBR_RS15695 overlap by 102 nucleotides
BBR_RS15690: BBR_RS15690 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase PurH, at 1,288,882 to 1,290,519
_RS15690
BBR_RS20920: BBR_RS20920 - hypothetical protein, at 1,290,674 to 1,290,976
_RS20920
BBR_RS15695: BBR_RS15695 - membrane channel protein, at 1,290,875 to 1,291,813
_RS15695
Position (kb)
1290
1291 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1290.643 kb on - strand at 1290.643 kb on - strand at 1290.677 kb on - strand at 1290.709 kb on + strand, within BBR_RS20920 at 1290.710 kb on + strand, within BBR_RS20920 at 1290.715 kb on + strand, within BBR_RS20920 at 1290.715 kb on + strand, within BBR_RS20920 at 1290.723 kb on - strand, within BBR_RS20920 at 1290.723 kb on - strand, within BBR_RS20920 at 1290.729 kb on + strand, within BBR_RS20920 at 1290.763 kb on + strand, within BBR_RS20920 at 1290.775 kb on + strand, within BBR_RS20920 at 1290.861 kb on - strand, within BBR_RS20920 at 1290.965 kb on + strand at 1290.972 kb on + strand at 1290.992 kb on - strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.127 kb on - strand, within BBR_RS15695 at 1291.127 kb on - strand, within BBR_RS15695 at 1291.198 kb on - strand, within BBR_RS15695 at 1291.250 kb on + strand, within BBR_RS15695 at 1291.258 kb on - strand, within BBR_RS15695 at 1291.373 kb on - strand, within BBR_RS15695 at 1291.379 kb on - strand, within BBR_RS15695 at 1291.446 kb on + strand, within BBR_RS15695 at 1291.510 kb on - strand, within BBR_RS15695 at 1291.561 kb on - strand, within BBR_RS15695 at 1291.561 kb on - strand, within BBR_RS15695 at 1291.658 kb on + strand, within BBR_RS15695 at 1291.658 kb on + strand, within BBR_RS15695 at 1291.677 kb on + strand, within BBR_RS15695 at 1291.682 kb on + strand, within BBR_RS15695 at 1291.697 kb on + strand, within BBR_RS15695 at 1291.710 kb on - strand, within BBR_RS15695 at 1291.720 kb on - strand at 1291.727 kb on - strand at 1291.735 kb on - strand at 1291.763 kb on - strand at 1291.791 kb on - strand at 1291.872 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,290,643 - -1.0 1,290,643 - -0.1 1,290,677 - -1.2 1,290,709 + BBR_RS20920 0.12 -0.3 1,290,710 + BBR_RS20920 0.12 -2.1 1,290,715 + BBR_RS20920 0.14 -1.0 1,290,715 + BBR_RS20920 0.14 -0.0 1,290,723 - BBR_RS20920 0.16 +0.1 1,290,723 - BBR_RS20920 0.16 -1.6 1,290,729 + BBR_RS20920 0.18 -1.8 1,290,763 + BBR_RS20920 0.29 -1.2 1,290,775 + BBR_RS20920 0.33 -0.9 1,290,861 - BBR_RS20920 0.62 -1.3 1,290,965 + -0.3 1,290,972 + -0.5 1,290,992 - BBR_RS15695 0.12 -0.3 1,291,041 + BBR_RS15695 0.18 -1.9 1,291,041 + BBR_RS15695 0.18 -0.4 1,291,041 + BBR_RS15695 0.18 -0.5 1,291,041 + BBR_RS15695 0.18 -1.2 1,291,041 + BBR_RS15695 0.18 +0.0 1,291,041 + BBR_RS15695 0.18 +0.8 1,291,041 + BBR_RS15695 0.18 -0.5 1,291,127 - BBR_RS15695 0.27 +1.3 1,291,127 - BBR_RS15695 0.27 +0.3 1,291,198 - BBR_RS15695 0.34 -0.7 1,291,250 + BBR_RS15695 0.40 -0.2 1,291,258 - BBR_RS15695 0.41 -1.8 1,291,373 - BBR_RS15695 0.53 +0.4 1,291,379 - BBR_RS15695 0.54 -1.5 1,291,446 + BBR_RS15695 0.61 +0.7 1,291,510 - BBR_RS15695 0.68 -0.9 1,291,561 - BBR_RS15695 0.73 +2.0 1,291,561 - BBR_RS15695 0.73 -0.2 1,291,658 + BBR_RS15695 0.83 +0.8 1,291,658 + BBR_RS15695 0.83 +0.4 1,291,677 + BBR_RS15695 0.85 -0.3 1,291,682 + BBR_RS15695 0.86 -0.3 1,291,697 + BBR_RS15695 0.88 -0.4 1,291,710 - BBR_RS15695 0.89 -0.2 1,291,720 - -0.4 1,291,727 - +0.1 1,291,735 - -1.1 1,291,763 - -2.2 1,291,791 - -0.2 1,291,872 + +0.7
Or see this region's nucleotide sequence