Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13410 and BBR_RS13415 are separated by 57 nucleotides BBR_RS13415 and BBR_RS20775 are separated by 157 nucleotides BBR_RS20775 and BBR_RS13420 are separated by 9 nucleotides BBR_RS13420 and BBR_RS13425 are separated by 126 nucleotides
BBR_RS13410: BBR_RS13410 - hypothetical protein, at 779,965 to 781,011
_RS13410
BBR_RS13415: BBR_RS13415 - hypothetical protein, at 781,069 to 781,701
_RS13415
BBR_RS20775: BBR_RS20775 - DUF4230 domain-containing protein, at 781,859 to 781,969
_RS20775
BBR_RS13420: BBR_RS13420 - DUF4230 domain-containing protein, at 781,979 to 782,266
_RS13420
BBR_RS13425: BBR_RS13425 - hypothetical protein, at 782,393 to 783,205
_RS13425
Position (kb) 781
782 Strain fitness (log2 ratio) -1
0
1 at 780.870 kb on + strand, within BBR_RS13410 at 780.883 kb on - strand, within BBR_RS13410 at 780.895 kb on - strand, within BBR_RS13410 at 780.895 kb on - strand, within BBR_RS13410 at 780.895 kb on - strand, within BBR_RS13410 at 780.980 kb on - strand at 781.027 kb on - strand at 781.034 kb on - strand at 781.107 kb on - strand at 781.138 kb on - strand, within BBR_RS13415 at 781.156 kb on + strand, within BBR_RS13415 at 781.164 kb on - strand, within BBR_RS13415 at 781.182 kb on + strand, within BBR_RS13415 at 781.182 kb on + strand, within BBR_RS13415 at 781.192 kb on - strand, within BBR_RS13415 at 781.244 kb on - strand, within BBR_RS13415 at 781.254 kb on - strand, within BBR_RS13415 at 781.269 kb on - strand, within BBR_RS13415 at 781.281 kb on + strand, within BBR_RS13415 at 781.297 kb on + strand, within BBR_RS13415 at 781.297 kb on + strand, within BBR_RS13415 at 781.305 kb on - strand, within BBR_RS13415 at 781.305 kb on - strand, within BBR_RS13415 at 781.305 kb on - strand, within BBR_RS13415 at 781.305 kb on - strand, within BBR_RS13415 at 781.305 kb on - strand, within BBR_RS13415 at 781.341 kb on - strand, within BBR_RS13415 at 781.367 kb on + strand, within BBR_RS13415 at 781.408 kb on + strand, within BBR_RS13415 at 781.408 kb on + strand, within BBR_RS13415 at 781.408 kb on + strand, within BBR_RS13415 at 781.413 kb on - strand, within BBR_RS13415 at 781.416 kb on - strand, within BBR_RS13415 at 781.416 kb on - strand, within BBR_RS13415 at 781.572 kb on + strand, within BBR_RS13415 at 781.597 kb on - strand, within BBR_RS13415 at 781.599 kb on - strand, within BBR_RS13415 at 781.599 kb on - strand, within BBR_RS13415 at 781.599 kb on - strand, within BBR_RS13415 at 781.628 kb on - strand, within BBR_RS13415 at 781.681 kb on + strand at 781.681 kb on - strand at 781.683 kb on - strand at 781.701 kb on - strand at 781.713 kb on - strand at 781.728 kb on - strand at 781.737 kb on - strand at 781.763 kb on - strand at 781.765 kb on - strand at 781.778 kb on + strand at 781.791 kb on + strand at 781.791 kb on + strand at 781.817 kb on + strand at 781.830 kb on - strand at 781.861 kb on + strand at 781.900 kb on - strand, within BBR_RS20775 at 781.941 kb on + strand, within BBR_RS20775 at 781.941 kb on + strand, within BBR_RS20775 at 782.070 kb on + strand, within BBR_RS13420 at 782.139 kb on - strand, within BBR_RS13420 at 782.155 kb on + strand, within BBR_RS13420 at 782.179 kb on + strand, within BBR_RS13420 at 782.210 kb on - strand, within BBR_RS13420 at 782.242 kb on + strand at 782.247 kb on - strand at 782.260 kb on - strand at 782.339 kb on - strand at 782.408 kb on - strand at 782.417 kb on + strand at 782.424 kb on - strand at 782.445 kb on + strand at 782.499 kb on + strand, within BBR_RS13425 at 782.499 kb on + strand, within BBR_RS13425 at 782.556 kb on + strand, within BBR_RS13425 at 782.676 kb on - strand, within BBR_RS13425 at 782.676 kb on - strand, within BBR_RS13425 at 782.765 kb on + strand, within BBR_RS13425 at 782.788 kb on + strand, within BBR_RS13425 at 782.789 kb on - strand, within BBR_RS13425 at 782.802 kb on - strand, within BBR_RS13425 at 782.919 kb on + strand, within BBR_RS13425 at 782.925 kb on - strand, within BBR_RS13425
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 780,870 + BBR_RS13410 0.86 +0.2 780,883 - BBR_RS13410 0.88 -0.0 780,895 - BBR_RS13410 0.89 +0.1 780,895 - BBR_RS13410 0.89 +0.2 780,895 - BBR_RS13410 0.89 -0.2 780,980 - +0.5 781,027 - +0.6 781,034 - +0.3 781,107 - -0.2 781,138 - BBR_RS13415 0.11 -0.1 781,156 + BBR_RS13415 0.14 -0.0 781,164 - BBR_RS13415 0.15 +0.0 781,182 + BBR_RS13415 0.18 +0.3 781,182 + BBR_RS13415 0.18 +0.3 781,192 - BBR_RS13415 0.19 -0.3 781,244 - BBR_RS13415 0.28 -0.5 781,254 - BBR_RS13415 0.29 -0.2 781,269 - BBR_RS13415 0.32 -0.6 781,281 + BBR_RS13415 0.33 +0.8 781,297 + BBR_RS13415 0.36 -0.1 781,297 + BBR_RS13415 0.36 +0.1 781,305 - BBR_RS13415 0.37 -1.2 781,305 - BBR_RS13415 0.37 +0.4 781,305 - BBR_RS13415 0.37 -0.3 781,305 - BBR_RS13415 0.37 -0.8 781,305 - BBR_RS13415 0.37 +0.7 781,341 - BBR_RS13415 0.43 +0.0 781,367 + BBR_RS13415 0.47 -0.2 781,408 + BBR_RS13415 0.54 -0.8 781,408 + BBR_RS13415 0.54 +0.2 781,408 + BBR_RS13415 0.54 +0.3 781,413 - BBR_RS13415 0.54 +0.5 781,416 - BBR_RS13415 0.55 -0.2 781,416 - BBR_RS13415 0.55 +0.7 781,572 + BBR_RS13415 0.79 +0.4 781,597 - BBR_RS13415 0.83 +0.4 781,599 - BBR_RS13415 0.84 +0.2 781,599 - BBR_RS13415 0.84 -0.1 781,599 - BBR_RS13415 0.84 +0.1 781,628 - BBR_RS13415 0.88 -0.3 781,681 + +0.3 781,681 - +0.8 781,683 - +0.5 781,701 - -0.0 781,713 - -0.6 781,728 - -0.7 781,737 - +0.3 781,763 - -0.7 781,765 - -0.0 781,778 + +0.4 781,791 + +0.5 781,791 + +0.8 781,817 + -0.0 781,830 - +0.6 781,861 + +0.5 781,900 - BBR_RS20775 0.37 -0.1 781,941 + BBR_RS20775 0.74 -0.4 781,941 + BBR_RS20775 0.74 -1.5 782,070 + BBR_RS13420 0.32 -0.4 782,139 - BBR_RS13420 0.56 +0.7 782,155 + BBR_RS13420 0.61 -0.2 782,179 + BBR_RS13420 0.69 +1.2 782,210 - BBR_RS13420 0.80 +0.4 782,242 + -0.0 782,247 - +0.7 782,260 - +0.1 782,339 - +0.3 782,408 - -0.2 782,417 + +0.8 782,424 - +0.1 782,445 + +0.0 782,499 + BBR_RS13425 0.13 +0.4 782,499 + BBR_RS13425 0.13 +0.9 782,556 + BBR_RS13425 0.20 -0.6 782,676 - BBR_RS13425 0.35 +0.3 782,676 - BBR_RS13425 0.35 +1.3 782,765 + BBR_RS13425 0.46 +0.4 782,788 + BBR_RS13425 0.49 +0.4 782,789 - BBR_RS13425 0.49 -0.5 782,802 - BBR_RS13425 0.50 +0.3 782,919 + BBR_RS13425 0.65 -0.2 782,925 - BBR_RS13425 0.65 +0.3
Or see this region's nucleotide sequence