Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS20720

Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS12605 and BBR_RS12610 overlap by 4 nucleotidesBBR_RS12610 and BBR_RS20720 overlap by 14 nucleotidesBBR_RS20720 and BBR_RS12620 are separated by 27 nucleotides BBR_RS12605: BBR_RS12605 - glycosyltransferase family 2 protein, at 584,085 to 585,095 _RS12605 BBR_RS12610: BBR_RS12610 - capsular polysaccharide biosynthesis protein, at 585,092 to 586,027 _RS12610 BBR_RS20720: BBR_RS20720 - DUF4422 domain-containing protein, at 586,014 to 586,844 _RS20720 BBR_RS12620: BBR_RS12620 - glycosyltransferase family 1 protein, at 586,872 to 587,945 _RS12620 Position (kb) 586 587Strain fitness (log2 ratio) -2 -1 0 1 2at 585.106 kb on + strandat 585.197 kb on - strand, within BBR_RS12610at 585.216 kb on - strand, within BBR_RS12610at 585.274 kb on - strand, within BBR_RS12610at 585.282 kb on - strand, within BBR_RS12610at 585.342 kb on + strand, within BBR_RS12610at 585.355 kb on - strand, within BBR_RS12610at 585.434 kb on + strand, within BBR_RS12610at 585.505 kb on + strand, within BBR_RS12610at 585.605 kb on - strand, within BBR_RS12610at 585.672 kb on - strand, within BBR_RS12610at 585.769 kb on - strand, within BBR_RS12610at 585.778 kb on + strand, within BBR_RS12610at 585.786 kb on - strand, within BBR_RS12610at 585.832 kb on + strand, within BBR_RS12610at 585.896 kb on - strand, within BBR_RS12610at 585.953 kb on - strandat 585.953 kb on - strandat 585.973 kb on - strandat 585.981 kb on + strandat 585.989 kb on - strandat 585.989 kb on - strandat 585.991 kb on - strandat 586.002 kb on + strandat 586.010 kb on - strandat 586.010 kb on - strandat 586.010 kb on - strandat 586.010 kb on - strandat 586.010 kb on - strandat 586.043 kb on - strandat 586.056 kb on - strandat 586.061 kb on + strandat 586.083 kb on - strandat 586.101 kb on - strand, within BBR_RS20720at 586.153 kb on + strand, within BBR_RS20720at 586.153 kb on + strand, within BBR_RS20720at 586.161 kb on - strand, within BBR_RS20720at 586.171 kb on + strand, within BBR_RS20720at 586.240 kb on - strand, within BBR_RS20720at 586.251 kb on - strand, within BBR_RS20720at 586.272 kb on - strand, within BBR_RS20720at 586.366 kb on + strand, within BBR_RS20720at 586.458 kb on - strand, within BBR_RS20720at 586.458 kb on - strand, within BBR_RS20720at 586.502 kb on + strand, within BBR_RS20720at 586.597 kb on + strand, within BBR_RS20720at 586.636 kb on - strand, within BBR_RS20720at 586.636 kb on - strand, within BBR_RS20720at 586.679 kb on - strand, within BBR_RS20720at 586.789 kb on + strandat 586.789 kb on - strandat 586.819 kb on - strandat 586.819 kb on - strandat 586.855 kb on - strandat 586.888 kb on + strandat 586.888 kb on + strandat 586.896 kb on - strandat 586.901 kb on - strandat 586.906 kb on + strandat 586.906 kb on - strandat 586.908 kb on + strandat 586.916 kb on - strandat 586.921 kb on - strandat 586.924 kb on - strandat 586.951 kb on + strandat 586.959 kb on - strandat 586.969 kb on - strandat 586.969 kb on - strandat 586.982 kb on - strand, within BBR_RS12620at 586.983 kb on - strand, within BBR_RS12620at 586.983 kb on - strand, within BBR_RS12620at 587.001 kb on - strand, within BBR_RS12620at 587.039 kb on - strand, within BBR_RS12620at 587.092 kb on - strand, within BBR_RS12620at 587.092 kb on - strand, within BBR_RS12620at 587.109 kb on + strand, within BBR_RS12620at 587.109 kb on + strand, within BBR_RS12620at 587.135 kb on - strand, within BBR_RS12620at 587.135 kb on - strand, within BBR_RS12620at 587.138 kb on + strand, within BBR_RS12620at 587.139 kb on + strand, within BBR_RS12620at 587.139 kb on + strand, within BBR_RS12620at 587.144 kb on + strand, within BBR_RS12620at 587.144 kb on + strand, within BBR_RS12620at 587.152 kb on - strand, within BBR_RS12620at 587.178 kb on - strand, within BBR_RS12620at 587.178 kb on - strand, within BBR_RS12620at 587.227 kb on - strand, within BBR_RS12620at 587.235 kb on + strand, within BBR_RS12620at 587.248 kb on + strand, within BBR_RS12620at 587.248 kb on + strand, within BBR_RS12620at 587.248 kb on + strand, within BBR_RS12620at 587.254 kb on + strand, within BBR_RS12620at 587.256 kb on - strand, within BBR_RS12620at 587.257 kb on - strand, within BBR_RS12620at 587.318 kb on - strand, within BBR_RS12620at 587.338 kb on - strand, within BBR_RS12620at 587.447 kb on + strand, within BBR_RS12620at 587.470 kb on - strand, within BBR_RS12620at 587.488 kb on + strand, within BBR_RS12620at 587.488 kb on + strand, within BBR_RS12620at 587.530 kb on + strand, within BBR_RS12620at 587.530 kb on + strand, within BBR_RS12620at 587.540 kb on + strand, within BBR_RS12620at 587.550 kb on + strand, within BBR_RS12620at 587.555 kb on + strand, within BBR_RS12620at 587.558 kb on + strand, within BBR_RS12620at 587.563 kb on + strand, within BBR_RS12620at 587.571 kb on - strand, within BBR_RS12620at 587.640 kb on - strand, within BBR_RS12620at 587.648 kb on - strand, within BBR_RS12620at 587.704 kb on - strand, within BBR_RS12620at 587.716 kb on - strand, within BBR_RS12620at 587.758 kb on - strand, within BBR_RS12620at 587.787 kb on + strand, within BBR_RS12620at 587.787 kb on + strand, within BBR_RS12620at 587.828 kb on - strand, within BBR_RS12620

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Per-strain Table

Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
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585,106 + +0.8
585,197 - BBR_RS12610 0.11 -0.0
585,216 - BBR_RS12610 0.13 +0.0
585,274 - BBR_RS12610 0.19 -0.7
585,282 - BBR_RS12610 0.20 -1.0
585,342 + BBR_RS12610 0.27 -1.3
585,355 - BBR_RS12610 0.28 +0.0
585,434 + BBR_RS12610 0.37 -1.1
585,505 + BBR_RS12610 0.44 +1.6
585,605 - BBR_RS12610 0.55 -0.5
585,672 - BBR_RS12610 0.62 -0.1
585,769 - BBR_RS12610 0.72 -0.3
585,778 + BBR_RS12610 0.73 +0.2
585,786 - BBR_RS12610 0.74 -0.7
585,832 + BBR_RS12610 0.79 -0.7
585,896 - BBR_RS12610 0.86 -0.3
585,953 - -1.0
585,953 - -0.3
585,973 - -1.2
585,981 + -0.9
585,989 - +1.7
585,989 - -2.0
585,991 - -1.2
586,002 + -0.8
586,010 - +0.2
586,010 - +0.2
586,010 - +0.6
586,010 - +0.4
586,010 - +0.2
586,043 - +0.2
586,056 - +1.7
586,061 + +1.6
586,083 - -0.3
586,101 - BBR_RS20720 0.10 -0.6
586,153 + BBR_RS20720 0.17 -0.8
586,153 + BBR_RS20720 0.17 +0.8
586,161 - BBR_RS20720 0.18 -0.9
586,171 + BBR_RS20720 0.19 +0.2
586,240 - BBR_RS20720 0.27 -1.1
586,251 - BBR_RS20720 0.29 -0.3
586,272 - BBR_RS20720 0.31 -0.4
586,366 + BBR_RS20720 0.42 +0.4
586,458 - BBR_RS20720 0.53 -1.2
586,458 - BBR_RS20720 0.53 +0.2
586,502 + BBR_RS20720 0.59 +0.1
586,597 + BBR_RS20720 0.70 -1.7
586,636 - BBR_RS20720 0.75 -0.4
586,636 - BBR_RS20720 0.75 -1.6
586,679 - BBR_RS20720 0.80 -1.4
586,789 + +1.6
586,789 - -0.4
586,819 - -0.5
586,819 - -1.3
586,855 - +0.1
586,888 + -0.8
586,888 + -0.5
586,896 - -0.5
586,901 - -0.7
586,906 + +0.3
586,906 - -0.1
586,908 + -1.2
586,916 - -0.3
586,921 - -0.8
586,924 - -0.6
586,951 + -0.5
586,959 - -0.4
586,969 - -0.6
586,969 - -0.6
586,982 - BBR_RS12620 0.10 +0.0
586,983 - BBR_RS12620 0.10 -0.4
586,983 - BBR_RS12620 0.10 -0.5
587,001 - BBR_RS12620 0.12 +0.2
587,039 - BBR_RS12620 0.16 +1.1
587,092 - BBR_RS12620 0.20 -0.1
587,092 - BBR_RS12620 0.20 -0.6
587,109 + BBR_RS12620 0.22 -0.2
587,109 + BBR_RS12620 0.22 -0.4
587,135 - BBR_RS12620 0.24 -0.1
587,135 - BBR_RS12620 0.24 -0.3
587,138 + BBR_RS12620 0.25 -0.6
587,139 + BBR_RS12620 0.25 +1.9
587,139 + BBR_RS12620 0.25 -0.6
587,144 + BBR_RS12620 0.25 +0.2
587,144 + BBR_RS12620 0.25 -0.9
587,152 - BBR_RS12620 0.26 +0.5
587,178 - BBR_RS12620 0.28 -1.5
587,178 - BBR_RS12620 0.28 -0.2
587,227 - BBR_RS12620 0.33 -0.6
587,235 + BBR_RS12620 0.34 +0.3
587,248 + BBR_RS12620 0.35 +0.3
587,248 + BBR_RS12620 0.35 +0.8
587,248 + BBR_RS12620 0.35 +0.2
587,254 + BBR_RS12620 0.36 +0.8
587,256 - BBR_RS12620 0.36 -0.7
587,257 - BBR_RS12620 0.36 +0.5
587,318 - BBR_RS12620 0.42 +0.2
587,338 - BBR_RS12620 0.43 +0.9
587,447 + BBR_RS12620 0.54 -0.7
587,470 - BBR_RS12620 0.56 +0.9
587,488 + BBR_RS12620 0.57 -0.3
587,488 + BBR_RS12620 0.57 +0.1
587,530 + BBR_RS12620 0.61 -0.3
587,530 + BBR_RS12620 0.61 +0.6
587,540 + BBR_RS12620 0.62 +0.0
587,550 + BBR_RS12620 0.63 +1.1
587,555 + BBR_RS12620 0.64 -0.4
587,558 + BBR_RS12620 0.64 -0.4
587,563 + BBR_RS12620 0.64 -0.7
587,571 - BBR_RS12620 0.65 -0.8
587,640 - BBR_RS12620 0.72 -0.9
587,648 - BBR_RS12620 0.72 -0.3
587,704 - BBR_RS12620 0.77 -1.3
587,716 - BBR_RS12620 0.79 -1.0
587,758 - BBR_RS12620 0.82 +0.0
587,787 + BBR_RS12620 0.85 +0.3
587,787 + BBR_RS12620 0.85 -0.3
587,828 - BBR_RS12620 0.89 +0.3

Or see this region's nucleotide sequence