Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19420 and BBR_RS19425 are separated by 32 nucleotides BBR_RS19425 and BBR_RS19430 are separated by 39 nucleotides BBR_RS19430 and BBR_RS19435 are separated by 214 nucleotides BBR_RS19435 and BBR_RS19440 are separated by 141 nucleotides
BBR_RS19420: BBR_RS19420 - transcription termination/antitermination factor NusG, at 2,140,906 to 2,141,733
_RS19420
BBR_RS19425: BBR_RS19425 - preprotein translocase subunit SecE, at 2,141,766 to 2,141,993
_RS19425
BBR_RS19430: BBR_RS19430 - tRNA-Trp, at 2,142,033 to 2,142,108
_RS19430
BBR_RS19435: BBR_RS19435 - pyridoxal phosphate-dependent aminotransferase, at 2,142,323 to 2,143,528
_RS19435
BBR_RS19440: BBR_RS19440 - polysaccharide deacetylase family protein, at 2,143,670 to 2,144,932
_RS19440
Position (kb)
2142
2143
2144 Strain fitness (log2 ratio)
-1
0
1
2 at 2142.209 kb on - strand at 2142.264 kb on - strand at 2142.268 kb on - strand at 2142.280 kb on + strand at 2142.280 kb on + strand at 2142.283 kb on - strand at 2142.283 kb on - strand at 2142.298 kb on + strand at 2142.298 kb on + strand at 2142.299 kb on - strand at 2142.304 kb on - strand at 2142.304 kb on - strand at 2142.304 kb on - strand at 2142.316 kb on - strand at 2142.322 kb on - strand at 2142.345 kb on - strand at 2142.353 kb on - strand at 2142.746 kb on + strand, within BBR_RS19435 at 2142.746 kb on + strand, within BBR_RS19435 at 2142.829 kb on - strand, within BBR_RS19435 at 2142.887 kb on + strand, within BBR_RS19435 at 2142.895 kb on - strand, within BBR_RS19435 at 2143.004 kb on + strand, within BBR_RS19435 at 2143.038 kb on - strand, within BBR_RS19435 at 2143.142 kb on + strand, within BBR_RS19435 at 2143.431 kb on - strand at 2143.455 kb on + strand at 2143.499 kb on - strand at 2143.499 kb on - strand at 2143.499 kb on - strand at 2143.529 kb on + strand at 2143.537 kb on - strand at 2143.549 kb on + strand at 2143.549 kb on + strand at 2143.557 kb on - strand at 2143.557 kb on - strand at 2143.557 kb on - strand at 2143.563 kb on + strand at 2143.563 kb on + strand at 2143.571 kb on - strand at 2143.571 kb on - strand at 2143.571 kb on - strand at 2143.571 kb on - strand at 2143.575 kb on + strand at 2143.586 kb on - strand at 2143.610 kb on + strand at 2143.618 kb on + strand at 2143.620 kb on + strand at 2143.626 kb on - strand at 2143.626 kb on - strand at 2143.626 kb on - strand at 2143.626 kb on - strand at 2143.631 kb on + strand at 2143.641 kb on + strand at 2143.649 kb on - strand at 2143.649 kb on - strand at 2143.649 kb on - strand at 2143.649 kb on - strand at 2143.656 kb on + strand at 2143.666 kb on + strand at 2143.688 kb on + strand at 2143.696 kb on - strand at 2143.719 kb on - strand at 2143.719 kb on - strand at 2143.719 kb on - strand at 2143.722 kb on - strand at 2143.725 kb on + strand at 2143.727 kb on - strand at 2143.729 kb on - strand at 2143.733 kb on - strand at 2143.738 kb on - strand at 2143.751 kb on - strand at 2143.777 kb on - strand at 2143.777 kb on - strand at 2143.786 kb on - strand at 2143.798 kb on - strand, within BBR_RS19440 at 2143.853 kb on + strand, within BBR_RS19440 at 2143.866 kb on - strand, within BBR_RS19440 at 2143.866 kb on - strand, within BBR_RS19440 at 2143.871 kb on - strand, within BBR_RS19440 at 2143.877 kb on + strand, within BBR_RS19440 at 2143.878 kb on - strand, within BBR_RS19440 at 2143.905 kb on + strand, within BBR_RS19440 at 2143.915 kb on + strand, within BBR_RS19440 at 2143.918 kb on - strand, within BBR_RS19440 at 2143.966 kb on - strand, within BBR_RS19440 at 2144.031 kb on + strand, within BBR_RS19440 at 2144.041 kb on - strand, within BBR_RS19440 at 2144.041 kb on - strand, within BBR_RS19440 at 2144.059 kb on + strand, within BBR_RS19440 at 2144.059 kb on + strand, within BBR_RS19440 at 2144.059 kb on + strand, within BBR_RS19440 at 2144.059 kb on + strand, within BBR_RS19440 at 2144.059 kb on + strand, within BBR_RS19440 at 2144.059 kb on + strand, within BBR_RS19440 at 2144.059 kb on + strand, within BBR_RS19440 at 2144.064 kb on + strand, within BBR_RS19440 at 2144.066 kb on + strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.067 kb on - strand, within BBR_RS19440 at 2144.074 kb on - strand, within BBR_RS19440 at 2144.096 kb on - strand, within BBR_RS19440 at 2144.111 kb on - strand, within BBR_RS19440 at 2144.122 kb on - strand, within BBR_RS19440 at 2144.125 kb on - strand, within BBR_RS19440 at 2144.125 kb on - strand, within BBR_RS19440 at 2144.155 kb on + strand, within BBR_RS19440 at 2144.156 kb on - strand, within BBR_RS19440 at 2144.163 kb on - strand, within BBR_RS19440 at 2144.171 kb on - strand, within BBR_RS19440 at 2144.178 kb on - strand, within BBR_RS19440 at 2144.180 kb on + strand, within BBR_RS19440 at 2144.180 kb on + strand, within BBR_RS19440 at 2144.180 kb on + strand, within BBR_RS19440 at 2144.180 kb on + strand, within BBR_RS19440 at 2144.180 kb on + strand, within BBR_RS19440 at 2144.182 kb on + strand, within BBR_RS19440 at 2144.182 kb on + strand, within BBR_RS19440 at 2144.182 kb on + strand, within BBR_RS19440 at 2144.188 kb on - strand, within BBR_RS19440 at 2144.188 kb on - strand, within BBR_RS19440 at 2144.188 kb on - strand, within BBR_RS19440 at 2144.188 kb on - strand, within BBR_RS19440 at 2144.188 kb on - strand, within BBR_RS19440 at 2144.190 kb on - strand, within BBR_RS19440 at 2144.192 kb on + strand, within BBR_RS19440 at 2144.192 kb on + strand, within BBR_RS19440 at 2144.200 kb on - strand, within BBR_RS19440 at 2144.200 kb on - strand, within BBR_RS19440 at 2144.200 kb on - strand, within BBR_RS19440 at 2144.200 kb on - strand, within BBR_RS19440 at 2144.216 kb on - strand, within BBR_RS19440 at 2144.216 kb on - strand, within BBR_RS19440 at 2144.238 kb on + strand, within BBR_RS19440 at 2144.238 kb on + strand, within BBR_RS19440 at 2144.241 kb on - strand, within BBR_RS19440 at 2144.293 kb on - strand, within BBR_RS19440 at 2144.293 kb on - strand, within BBR_RS19440 at 2144.342 kb on + strand, within BBR_RS19440 at 2144.342 kb on + strand, within BBR_RS19440 at 2144.349 kb on - strand, within BBR_RS19440 at 2144.349 kb on - strand, within BBR_RS19440 at 2144.384 kb on - strand, within BBR_RS19440 at 2144.403 kb on - strand, within BBR_RS19440 at 2144.409 kb on + strand, within BBR_RS19440 at 2144.409 kb on + strand, within BBR_RS19440 at 2144.409 kb on + strand, within BBR_RS19440 at 2144.409 kb on + strand, within BBR_RS19440 at 2144.417 kb on - strand, within BBR_RS19440 at 2144.417 kb on - strand, within BBR_RS19440 at 2144.417 kb on - strand, within BBR_RS19440 at 2144.417 kb on - strand, within BBR_RS19440 at 2144.419 kb on - strand, within BBR_RS19440 at 2144.432 kb on - strand, within BBR_RS19440 at 2144.443 kb on - strand, within BBR_RS19440 at 2144.443 kb on - strand, within BBR_RS19440 at 2144.443 kb on - strand, within BBR_RS19440 at 2144.450 kb on + strand, within BBR_RS19440 at 2144.472 kb on + strand, within BBR_RS19440 at 2144.496 kb on + strand, within BBR_RS19440 at 2144.503 kb on + strand, within BBR_RS19440 at 2144.511 kb on + strand, within BBR_RS19440 at 2144.511 kb on - strand, within BBR_RS19440 at 2144.511 kb on - strand, within BBR_RS19440 at 2144.516 kb on + strand, within BBR_RS19440 at 2144.527 kb on + strand, within BBR_RS19440
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 2,142,209 - +0.4 2,142,264 - +0.0 2,142,268 - +0.4 2,142,280 + +0.3 2,142,280 + +0.6 2,142,283 - +0.7 2,142,283 - -0.2 2,142,298 + +0.4 2,142,298 + +0.1 2,142,299 - -0.2 2,142,304 - +0.1 2,142,304 - +0.4 2,142,304 - -1.0 2,142,316 - -0.5 2,142,322 - -0.5 2,142,345 - +0.6 2,142,353 - -0.8 2,142,746 + BBR_RS19435 0.35 +1.4 2,142,746 + BBR_RS19435 0.35 -0.2 2,142,829 - BBR_RS19435 0.42 +0.8 2,142,887 + BBR_RS19435 0.47 -0.8 2,142,895 - BBR_RS19435 0.47 -0.0 2,143,004 + BBR_RS19435 0.56 -0.6 2,143,038 - BBR_RS19435 0.59 +0.4 2,143,142 + BBR_RS19435 0.68 +0.8 2,143,431 - +1.8 2,143,455 + -0.4 2,143,499 - -0.3 2,143,499 - +0.0 2,143,499 - +0.2 2,143,529 + +0.0 2,143,537 - -0.6 2,143,549 + +0.5 2,143,549 + -0.1 2,143,557 - -0.0 2,143,557 - -0.5 2,143,557 - +0.1 2,143,563 + +0.5 2,143,563 + -1.3 2,143,571 - -0.6 2,143,571 - +0.4 2,143,571 - -0.5 2,143,571 - +0.8 2,143,575 + +0.7 2,143,586 - +0.8 2,143,610 + +0.2 2,143,618 + -0.3 2,143,620 + -0.4 2,143,626 - +0.5 2,143,626 - -0.5 2,143,626 - -0.5 2,143,626 - +0.2 2,143,631 + +1.0 2,143,641 + -0.8 2,143,649 - +0.5 2,143,649 - +0.2 2,143,649 - +0.0 2,143,649 - -0.1 2,143,656 + -0.1 2,143,666 + +1.1 2,143,688 + -0.2 2,143,696 - -0.6 2,143,719 - +0.8 2,143,719 - +1.5 2,143,719 - +0.7 2,143,722 - +0.3 2,143,725 + +1.1 2,143,727 - -0.6 2,143,729 - +0.2 2,143,733 - -0.4 2,143,738 - +0.0 2,143,751 - +0.1 2,143,777 - +0.6 2,143,777 - -0.4 2,143,786 - +0.2 2,143,798 - BBR_RS19440 0.10 -0.4 2,143,853 + BBR_RS19440 0.14 +0.7 2,143,866 - BBR_RS19440 0.16 +0.5 2,143,866 - BBR_RS19440 0.16 -0.0 2,143,871 - BBR_RS19440 0.16 -0.4 2,143,877 + BBR_RS19440 0.16 +0.3 2,143,878 - BBR_RS19440 0.16 -0.4 2,143,905 + BBR_RS19440 0.19 +0.9 2,143,915 + BBR_RS19440 0.19 +1.2 2,143,918 - BBR_RS19440 0.20 +0.4 2,143,966 - BBR_RS19440 0.23 -0.9 2,144,031 + BBR_RS19440 0.29 -0.8 2,144,041 - BBR_RS19440 0.29 +0.2 2,144,041 - BBR_RS19440 0.29 -0.3 2,144,059 + BBR_RS19440 0.31 +0.0 2,144,059 + BBR_RS19440 0.31 +0.4 2,144,059 + BBR_RS19440 0.31 +0.1 2,144,059 + BBR_RS19440 0.31 -0.8 2,144,059 + BBR_RS19440 0.31 +0.5 2,144,059 + BBR_RS19440 0.31 -0.7 2,144,059 + BBR_RS19440 0.31 +0.7 2,144,064 + BBR_RS19440 0.31 +0.2 2,144,066 + BBR_RS19440 0.31 +0.8 2,144,067 - BBR_RS19440 0.31 -0.5 2,144,067 - BBR_RS19440 0.31 +0.6 2,144,067 - BBR_RS19440 0.31 -0.2 2,144,067 - BBR_RS19440 0.31 +0.3 2,144,067 - BBR_RS19440 0.31 -0.5 2,144,067 - BBR_RS19440 0.31 -0.1 2,144,067 - BBR_RS19440 0.31 +1.1 2,144,067 - BBR_RS19440 0.31 -0.1 2,144,067 - BBR_RS19440 0.31 -0.4 2,144,067 - BBR_RS19440 0.31 +0.9 2,144,074 - BBR_RS19440 0.32 -0.1 2,144,096 - BBR_RS19440 0.34 -0.0 2,144,111 - BBR_RS19440 0.35 +0.4 2,144,122 - BBR_RS19440 0.36 +0.7 2,144,125 - BBR_RS19440 0.36 -0.2 2,144,125 - BBR_RS19440 0.36 -0.5 2,144,155 + BBR_RS19440 0.38 +0.9 2,144,156 - BBR_RS19440 0.38 +0.2 2,144,163 - BBR_RS19440 0.39 -0.1 2,144,171 - BBR_RS19440 0.40 -0.3 2,144,178 - BBR_RS19440 0.40 -0.3 2,144,180 + BBR_RS19440 0.40 +0.3 2,144,180 + BBR_RS19440 0.40 +0.0 2,144,180 + BBR_RS19440 0.40 +0.2 2,144,180 + BBR_RS19440 0.40 +0.7 2,144,180 + BBR_RS19440 0.40 +0.1 2,144,182 + BBR_RS19440 0.41 +0.2 2,144,182 + BBR_RS19440 0.41 +0.3 2,144,182 + BBR_RS19440 0.41 +0.0 2,144,188 - BBR_RS19440 0.41 -1.5 2,144,188 - BBR_RS19440 0.41 +0.2 2,144,188 - BBR_RS19440 0.41 -0.1 2,144,188 - BBR_RS19440 0.41 +0.6 2,144,188 - BBR_RS19440 0.41 +0.6 2,144,190 - BBR_RS19440 0.41 -0.1 2,144,192 + BBR_RS19440 0.41 +0.6 2,144,192 + BBR_RS19440 0.41 +0.0 2,144,200 - BBR_RS19440 0.42 +0.5 2,144,200 - BBR_RS19440 0.42 +0.7 2,144,200 - BBR_RS19440 0.42 +0.1 2,144,200 - BBR_RS19440 0.42 -1.4 2,144,216 - BBR_RS19440 0.43 +0.5 2,144,216 - BBR_RS19440 0.43 -1.0 2,144,238 + BBR_RS19440 0.45 +1.1 2,144,238 + BBR_RS19440 0.45 +0.5 2,144,241 - BBR_RS19440 0.45 +0.1 2,144,293 - BBR_RS19440 0.49 +0.1 2,144,293 - BBR_RS19440 0.49 +0.1 2,144,342 + BBR_RS19440 0.53 +1.2 2,144,342 + BBR_RS19440 0.53 -0.3 2,144,349 - BBR_RS19440 0.54 +0.4 2,144,349 - BBR_RS19440 0.54 +0.2 2,144,384 - BBR_RS19440 0.57 +0.8 2,144,403 - BBR_RS19440 0.58 +0.3 2,144,409 + BBR_RS19440 0.59 +0.9 2,144,409 + BBR_RS19440 0.59 +0.2 2,144,409 + BBR_RS19440 0.59 +0.5 2,144,409 + BBR_RS19440 0.59 +0.6 2,144,417 - BBR_RS19440 0.59 -0.0 2,144,417 - BBR_RS19440 0.59 -0.7 2,144,417 - BBR_RS19440 0.59 +0.7 2,144,417 - BBR_RS19440 0.59 -0.1 2,144,419 - BBR_RS19440 0.59 +0.6 2,144,432 - BBR_RS19440 0.60 +0.0 2,144,443 - BBR_RS19440 0.61 -0.0 2,144,443 - BBR_RS19440 0.61 -0.2 2,144,443 - BBR_RS19440 0.61 +1.0 2,144,450 + BBR_RS19440 0.62 +0.9 2,144,472 + BBR_RS19440 0.63 -0.3 2,144,496 + BBR_RS19440 0.65 +0.1 2,144,503 + BBR_RS19440 0.66 +0.9 2,144,511 + BBR_RS19440 0.67 +0.7 2,144,511 - BBR_RS19440 0.67 +0.2 2,144,511 - BBR_RS19440 0.67 +1.6 2,144,516 + BBR_RS19440 0.67 +1.3 2,144,527 + BBR_RS19440 0.68 -0.2
Or see this region's nucleotide sequence