Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19120 and BBR_RS19125 are separated by 8 nucleotides BBR_RS19125 and BBR_RS19130 are separated by 258 nucleotides BBR_RS19130 and BBR_RS19135 are separated by 109 nucleotides
BBR_RS19120: BBR_RS19120 - helicase, at 2,061,587 to 2,065,207
_RS19120
BBR_RS19125: BBR_RS19125 - hypothetical protein, at 2,065,216 to 2,066,526
_RS19125
BBR_RS19130: BBR_RS19130 - 50S ribosomal protein L7/L12, at 2,066,785 to 2,067,165
_RS19130
BBR_RS19135: BBR_RS19135 - 50S ribosomal protein L10, at 2,067,275 to 2,067,796
_RS19135
Position (kb)
2065
2066
2067 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2064.314 kb on - strand, within BBR_RS19120 at 2064.396 kb on + strand, within BBR_RS19120 at 2064.404 kb on - strand, within BBR_RS19120 at 2064.404 kb on - strand, within BBR_RS19120 at 2064.404 kb on - strand, within BBR_RS19120 at 2064.413 kb on - strand, within BBR_RS19120 at 2064.447 kb on + strand, within BBR_RS19120 at 2064.455 kb on - strand, within BBR_RS19120 at 2064.455 kb on - strand, within BBR_RS19120 at 2064.455 kb on - strand, within BBR_RS19120 at 2064.460 kb on - strand, within BBR_RS19120 at 2064.539 kb on - strand, within BBR_RS19120 at 2064.563 kb on - strand, within BBR_RS19120 at 2064.608 kb on - strand, within BBR_RS19120 at 2064.635 kb on - strand, within BBR_RS19120 at 2064.637 kb on - strand, within BBR_RS19120 at 2064.679 kb on - strand, within BBR_RS19120 at 2064.695 kb on - strand, within BBR_RS19120 at 2064.696 kb on - strand, within BBR_RS19120 at 2064.717 kb on - strand, within BBR_RS19120 at 2064.742 kb on + strand, within BBR_RS19120 at 2064.742 kb on + strand, within BBR_RS19120 at 2064.750 kb on - strand, within BBR_RS19120 at 2064.750 kb on - strand, within BBR_RS19120 at 2064.790 kb on + strand, within BBR_RS19120 at 2064.795 kb on - strand, within BBR_RS19120 at 2064.803 kb on + strand, within BBR_RS19120 at 2064.852 kb on + strand at 2064.860 kb on - strand at 2064.860 kb on - strand at 2064.873 kb on + strand at 2064.881 kb on - strand at 2064.903 kb on - strand at 2064.905 kb on - strand at 2064.957 kb on - strand at 2064.960 kb on + strand at 2064.962 kb on - strand at 2064.992 kb on - strand at 2065.000 kb on - strand at 2065.002 kb on + strand at 2065.061 kb on - strand at 2065.071 kb on - strand at 2065.085 kb on - strand at 2065.205 kb on - strand at 2065.208 kb on + strand at 2065.219 kb on + strand at 2065.221 kb on + strand at 2065.221 kb on + strand at 2065.227 kb on - strand at 2065.239 kb on + strand at 2065.255 kb on - strand at 2065.261 kb on + strand at 2065.261 kb on + strand at 2065.269 kb on - strand at 2065.269 kb on - strand at 2065.269 kb on - strand at 2065.269 kb on - strand at 2065.269 kb on - strand at 2065.271 kb on - strand at 2065.282 kb on + strand at 2065.316 kb on - strand at 2065.318 kb on - strand at 2065.326 kb on + strand at 2065.326 kb on + strand at 2065.326 kb on + strand at 2065.326 kb on + strand at 2065.364 kb on - strand, within BBR_RS19125 at 2065.364 kb on - strand, within BBR_RS19125 at 2065.364 kb on - strand, within BBR_RS19125 at 2065.371 kb on - strand, within BBR_RS19125 at 2065.381 kb on - strand, within BBR_RS19125 at 2065.381 kb on - strand, within BBR_RS19125 at 2065.390 kb on + strand, within BBR_RS19125 at 2065.464 kb on - strand, within BBR_RS19125 at 2065.464 kb on - strand, within BBR_RS19125 at 2065.478 kb on + strand, within BBR_RS19125 at 2065.488 kb on - strand, within BBR_RS19125 at 2065.488 kb on - strand, within BBR_RS19125 at 2065.488 kb on - strand, within BBR_RS19125 at 2065.494 kb on - strand, within BBR_RS19125 at 2065.516 kb on - strand, within BBR_RS19125 at 2065.516 kb on - strand, within BBR_RS19125 at 2065.521 kb on - strand, within BBR_RS19125 at 2065.526 kb on - strand, within BBR_RS19125 at 2065.546 kb on - strand, within BBR_RS19125 at 2065.563 kb on - strand, within BBR_RS19125 at 2065.581 kb on - strand, within BBR_RS19125 at 2065.581 kb on - strand, within BBR_RS19125 at 2065.595 kb on - strand, within BBR_RS19125 at 2065.630 kb on - strand, within BBR_RS19125 at 2065.637 kb on + strand, within BBR_RS19125 at 2065.640 kb on - strand, within BBR_RS19125 at 2065.640 kb on - strand, within BBR_RS19125 at 2065.645 kb on - strand, within BBR_RS19125 at 2065.653 kb on - strand, within BBR_RS19125 at 2065.658 kb on - strand, within BBR_RS19125 at 2065.675 kb on - strand, within BBR_RS19125 at 2065.675 kb on - strand, within BBR_RS19125 at 2065.681 kb on - strand, within BBR_RS19125 at 2065.735 kb on + strand, within BBR_RS19125 at 2065.741 kb on - strand, within BBR_RS19125 at 2065.745 kb on - strand, within BBR_RS19125 at 2065.745 kb on - strand, within BBR_RS19125 at 2065.753 kb on - strand, within BBR_RS19125 at 2065.753 kb on - strand, within BBR_RS19125 at 2065.774 kb on - strand, within BBR_RS19125 at 2065.778 kb on - strand, within BBR_RS19125 at 2065.779 kb on + strand, within BBR_RS19125 at 2065.797 kb on - strand, within BBR_RS19125 at 2065.800 kb on + strand, within BBR_RS19125 at 2065.808 kb on - strand, within BBR_RS19125 at 2065.808 kb on - strand, within BBR_RS19125 at 2065.876 kb on - strand, within BBR_RS19125 at 2065.876 kb on - strand, within BBR_RS19125 at 2065.881 kb on - strand, within BBR_RS19125 at 2065.889 kb on - strand, within BBR_RS19125 at 2065.955 kb on - strand, within BBR_RS19125 at 2065.957 kb on - strand, within BBR_RS19125 at 2065.967 kb on - strand, within BBR_RS19125 at 2065.978 kb on - strand, within BBR_RS19125 at 2066.023 kb on - strand, within BBR_RS19125 at 2066.097 kb on - strand, within BBR_RS19125 at 2066.169 kb on - strand, within BBR_RS19125 at 2066.199 kb on - strand, within BBR_RS19125 at 2066.199 kb on - strand, within BBR_RS19125 at 2066.210 kb on + strand, within BBR_RS19125 at 2066.218 kb on - strand, within BBR_RS19125 at 2066.252 kb on - strand, within BBR_RS19125 at 2066.252 kb on - strand, within BBR_RS19125 at 2066.252 kb on - strand, within BBR_RS19125 at 2066.303 kb on - strand, within BBR_RS19125 at 2066.313 kb on - strand, within BBR_RS19125 at 2066.324 kb on - strand, within BBR_RS19125 at 2066.357 kb on - strand, within BBR_RS19125 at 2066.362 kb on - strand, within BBR_RS19125 at 2066.364 kb on - strand, within BBR_RS19125 at 2066.397 kb on - strand at 2066.403 kb on + strand at 2066.411 kb on - strand at 2066.411 kb on - strand at 2066.431 kb on - strand at 2066.503 kb on + strand at 2066.503 kb on - strand at 2066.503 kb on - strand at 2066.511 kb on - strand at 2066.511 kb on - strand at 2066.513 kb on + strand at 2066.538 kb on + strand at 2066.539 kb on + strand at 2066.539 kb on + strand at 2066.545 kb on - strand at 2066.545 kb on - strand at 2066.545 kb on - strand at 2066.547 kb on + strand at 2066.547 kb on - strand at 2066.547 kb on - strand at 2066.547 kb on - strand at 2066.547 kb on - strand at 2066.553 kb on + strand at 2066.557 kb on - strand at 2066.561 kb on - strand at 2066.561 kb on - strand at 2066.565 kb on + strand at 2066.565 kb on + strand at 2066.569 kb on - strand at 2066.571 kb on - strand at 2066.573 kb on - strand at 2066.580 kb on + strand at 2066.648 kb on - strand at 2066.668 kb on + strand at 2066.673 kb on + strand at 2066.673 kb on + strand at 2066.673 kb on + strand at 2066.673 kb on + strand at 2066.673 kb on + strand at 2066.673 kb on + strand at 2066.678 kb on + strand at 2066.678 kb on + strand at 2066.678 kb on + strand at 2066.678 kb on + strand at 2066.681 kb on - strand at 2066.683 kb on + strand at 2066.689 kb on + strand at 2066.694 kb on + strand at 2066.694 kb on + strand at 2066.694 kb on + strand at 2066.694 kb on + strand at 2066.702 kb on - strand at 2066.707 kb on + strand at 2066.708 kb on - strand at 2066.715 kb on - strand at 2066.732 kb on - strand at 2066.742 kb on - strand at 2066.742 kb on - strand at 2066.742 kb on - strand at 2066.762 kb on - strand at 2066.776 kb on - strand at 2066.786 kb on - strand at 2066.786 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 2,064,314 - BBR_RS19120 0.75 -3.6 2,064,396 + BBR_RS19120 0.78 +0.1 2,064,404 - BBR_RS19120 0.78 -3.3 2,064,404 - BBR_RS19120 0.78 -2.7 2,064,404 - BBR_RS19120 0.78 +1.4 2,064,413 - BBR_RS19120 0.78 -3.0 2,064,447 + BBR_RS19120 0.79 -2.1 2,064,455 - BBR_RS19120 0.79 -0.9 2,064,455 - BBR_RS19120 0.79 -1.3 2,064,455 - BBR_RS19120 0.79 -3.2 2,064,460 - BBR_RS19120 0.79 +0.8 2,064,539 - BBR_RS19120 0.82 -1.8 2,064,563 - BBR_RS19120 0.82 -1.6 2,064,608 - BBR_RS19120 0.83 -1.2 2,064,635 - BBR_RS19120 0.84 -1.2 2,064,637 - BBR_RS19120 0.84 -2.1 2,064,679 - BBR_RS19120 0.85 -1.7 2,064,695 - BBR_RS19120 0.86 -1.4 2,064,696 - BBR_RS19120 0.86 -3.4 2,064,717 - BBR_RS19120 0.86 -0.9 2,064,742 + BBR_RS19120 0.87 -0.8 2,064,742 + BBR_RS19120 0.87 -2.9 2,064,750 - BBR_RS19120 0.87 -2.1 2,064,750 - BBR_RS19120 0.87 -1.5 2,064,790 + BBR_RS19120 0.88 -0.9 2,064,795 - BBR_RS19120 0.89 -1.4 2,064,803 + BBR_RS19120 0.89 +1.4 2,064,852 + +0.8 2,064,860 - -1.2 2,064,860 - -1.1 2,064,873 + -2.7 2,064,881 - -0.6 2,064,903 - -1.8 2,064,905 - -1.8 2,064,957 - -2.1 2,064,960 + +1.4 2,064,962 - -1.6 2,064,992 - -0.5 2,065,000 - -1.2 2,065,002 + +1.4 2,065,061 - -1.2 2,065,071 - -0.2 2,065,085 - -1.7 2,065,205 - -0.6 2,065,208 + -2.1 2,065,219 + -0.8 2,065,221 + -0.2 2,065,221 + -1.2 2,065,227 - -0.7 2,065,239 + -2.3 2,065,255 - +0.8 2,065,261 + -0.2 2,065,261 + +0.1 2,065,269 - +0.1 2,065,269 - -2.1 2,065,269 - -0.4 2,065,269 - -1.3 2,065,269 - +0.4 2,065,271 - -0.8 2,065,282 + -1.0 2,065,316 - -3.0 2,065,318 - -3.5 2,065,326 + -0.6 2,065,326 + -2.5 2,065,326 + -0.8 2,065,326 + -0.6 2,065,364 - BBR_RS19125 0.11 -1.8 2,065,364 - BBR_RS19125 0.11 -1.8 2,065,364 - BBR_RS19125 0.11 -2.1 2,065,371 - BBR_RS19125 0.12 +1.0 2,065,381 - BBR_RS19125 0.13 -1.7 2,065,381 - BBR_RS19125 0.13 -1.1 2,065,390 + BBR_RS19125 0.13 -0.8 2,065,464 - BBR_RS19125 0.19 -2.1 2,065,464 - BBR_RS19125 0.19 -0.9 2,065,478 + BBR_RS19125 0.20 -1.5 2,065,488 - BBR_RS19125 0.21 -1.7 2,065,488 - BBR_RS19125 0.21 -2.2 2,065,488 - BBR_RS19125 0.21 -0.4 2,065,494 - BBR_RS19125 0.21 -1.2 2,065,516 - BBR_RS19125 0.23 -3.0 2,065,516 - BBR_RS19125 0.23 -1.9 2,065,521 - BBR_RS19125 0.23 -2.0 2,065,526 - BBR_RS19125 0.24 -1.0 2,065,546 - BBR_RS19125 0.25 -2.5 2,065,563 - BBR_RS19125 0.26 -1.6 2,065,581 - BBR_RS19125 0.28 -0.3 2,065,581 - BBR_RS19125 0.28 +0.1 2,065,595 - BBR_RS19125 0.29 -1.8 2,065,630 - BBR_RS19125 0.32 -2.1 2,065,637 + BBR_RS19125 0.32 +0.8 2,065,640 - BBR_RS19125 0.32 -1.8 2,065,640 - BBR_RS19125 0.32 -2.7 2,065,645 - BBR_RS19125 0.33 +0.1 2,065,653 - BBR_RS19125 0.33 -0.7 2,065,658 - BBR_RS19125 0.34 -1.6 2,065,675 - BBR_RS19125 0.35 -1.0 2,065,675 - BBR_RS19125 0.35 -0.9 2,065,681 - BBR_RS19125 0.35 -0.8 2,065,735 + BBR_RS19125 0.40 +2.4 2,065,741 - BBR_RS19125 0.40 -1.2 2,065,745 - BBR_RS19125 0.40 -1.2 2,065,745 - BBR_RS19125 0.40 -1.7 2,065,753 - BBR_RS19125 0.41 -2.2 2,065,753 - BBR_RS19125 0.41 -0.8 2,065,774 - BBR_RS19125 0.43 -2.2 2,065,778 - BBR_RS19125 0.43 -2.4 2,065,779 + BBR_RS19125 0.43 -0.2 2,065,797 - BBR_RS19125 0.44 -2.1 2,065,800 + BBR_RS19125 0.45 +2.4 2,065,808 - BBR_RS19125 0.45 -0.5 2,065,808 - BBR_RS19125 0.45 +0.5 2,065,876 - BBR_RS19125 0.50 -3.1 2,065,876 - BBR_RS19125 0.50 -2.6 2,065,881 - BBR_RS19125 0.51 -2.0 2,065,889 - BBR_RS19125 0.51 -1.2 2,065,955 - BBR_RS19125 0.56 -1.3 2,065,957 - BBR_RS19125 0.57 -2.1 2,065,967 - BBR_RS19125 0.57 -0.4 2,065,978 - BBR_RS19125 0.58 -0.1 2,066,023 - BBR_RS19125 0.62 +0.1 2,066,097 - BBR_RS19125 0.67 +0.6 2,066,169 - BBR_RS19125 0.73 -1.7 2,066,199 - BBR_RS19125 0.75 -3.3 2,066,199 - BBR_RS19125 0.75 -2.9 2,066,210 + BBR_RS19125 0.76 -1.7 2,066,218 - BBR_RS19125 0.76 -1.8 2,066,252 - BBR_RS19125 0.79 -2.9 2,066,252 - BBR_RS19125 0.79 -2.5 2,066,252 - BBR_RS19125 0.79 +0.7 2,066,303 - BBR_RS19125 0.83 +0.6 2,066,313 - BBR_RS19125 0.84 -1.7 2,066,324 - BBR_RS19125 0.85 -2.9 2,066,357 - BBR_RS19125 0.87 -1.0 2,066,362 - BBR_RS19125 0.87 -2.6 2,066,364 - BBR_RS19125 0.88 -2.8 2,066,397 - -1.4 2,066,403 + +1.4 2,066,411 - -1.4 2,066,411 - -2.9 2,066,431 - -0.9 2,066,503 + +0.8 2,066,503 - -0.1 2,066,503 - -1.3 2,066,511 - -1.3 2,066,511 - -2.1 2,066,513 + -2.2 2,066,538 + -0.8 2,066,539 + +0.1 2,066,539 + -0.6 2,066,545 - -0.4 2,066,545 - -0.0 2,066,545 - -0.4 2,066,547 + -0.4 2,066,547 - +0.2 2,066,547 - -0.8 2,066,547 - -0.7 2,066,547 - -0.2 2,066,553 + -0.8 2,066,557 - -0.5 2,066,561 - -1.2 2,066,561 - -0.8 2,066,565 + -2.2 2,066,565 + -1.8 2,066,569 - -1.5 2,066,571 - -1.3 2,066,573 - -0.1 2,066,580 + +0.2 2,066,648 - -0.1 2,066,668 + +1.1 2,066,673 + -0.1 2,066,673 + +0.5 2,066,673 + +0.3 2,066,673 + +0.5 2,066,673 + +1.3 2,066,673 + -0.2 2,066,678 + +0.4 2,066,678 + +0.2 2,066,678 + +0.7 2,066,678 + +0.3 2,066,681 - +0.2 2,066,683 + +0.2 2,066,689 + +1.1 2,066,694 + +0.3 2,066,694 + -0.5 2,066,694 + -0.4 2,066,694 + -1.0 2,066,702 - +0.2 2,066,707 + -0.6 2,066,708 - +1.1 2,066,715 - -0.8 2,066,732 - -0.1 2,066,742 - -1.2 2,066,742 - -0.2 2,066,742 - -0.1 2,066,762 - +0.1 2,066,776 - -0.2 2,066,786 - -0.1 2,066,786 - -0.1
Or see this region's nucleotide sequence