Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18720 and BBR_RS18725 are separated by 54 nucleotides BBR_RS18725 and BBR_RS18730 are separated by 130 nucleotides
BBR_RS18720: BBR_RS18720 - pyridine nucleotide-disulfide oxidoreductase, at 1,990,160 to 1,991,668
_RS18720
BBR_RS18725: BBR_RS18725 - ribonuclease HI, at 1,991,723 to 1,992,820
_RS18725
BBR_RS18730: BBR_RS18730 - ribose-5-phosphate isomerase RpiA, at 1,992,951 to 1,993,649
_RS18730
Position (kb)
1991
1992
1993 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1990.724 kb on + strand, within BBR_RS18720 at 1990.727 kb on - strand, within BBR_RS18720 at 1990.732 kb on - strand, within BBR_RS18720 at 1990.733 kb on + strand, within BBR_RS18720 at 1990.770 kb on - strand, within BBR_RS18720 at 1990.772 kb on + strand, within BBR_RS18720 at 1990.784 kb on + strand, within BBR_RS18720 at 1990.784 kb on + strand, within BBR_RS18720 at 1990.789 kb on + strand, within BBR_RS18720 at 1990.792 kb on - strand, within BBR_RS18720 at 1990.792 kb on - strand, within BBR_RS18720 at 1990.792 kb on - strand, within BBR_RS18720 at 1990.817 kb on + strand, within BBR_RS18720 at 1990.847 kb on + strand, within BBR_RS18720 at 1990.857 kb on - strand, within BBR_RS18720 at 1990.857 kb on - strand, within BBR_RS18720 at 1990.863 kb on - strand, within BBR_RS18720 at 1990.891 kb on - strand, within BBR_RS18720 at 1990.912 kb on + strand, within BBR_RS18720 at 1990.933 kb on - strand, within BBR_RS18720 at 1990.940 kb on + strand, within BBR_RS18720 at 1990.945 kb on + strand, within BBR_RS18720 at 1990.957 kb on + strand, within BBR_RS18720 at 1990.965 kb on - strand, within BBR_RS18720 at 1990.970 kb on - strand, within BBR_RS18720 at 1990.995 kb on + strand, within BBR_RS18720 at 1991.000 kb on + strand, within BBR_RS18720 at 1991.019 kb on + strand, within BBR_RS18720 at 1991.022 kb on - strand, within BBR_RS18720 at 1991.022 kb on - strand, within BBR_RS18720 at 1991.029 kb on + strand, within BBR_RS18720 at 1991.032 kb on - strand, within BBR_RS18720 at 1991.040 kb on - strand, within BBR_RS18720 at 1991.049 kb on + strand, within BBR_RS18720 at 1991.049 kb on + strand, within BBR_RS18720 at 1991.049 kb on - strand, within BBR_RS18720 at 1991.052 kb on - strand, within BBR_RS18720 at 1991.060 kb on + strand, within BBR_RS18720 at 1991.101 kb on - strand, within BBR_RS18720 at 1991.101 kb on - strand, within BBR_RS18720 at 1991.121 kb on - strand, within BBR_RS18720 at 1991.142 kb on + strand, within BBR_RS18720 at 1991.150 kb on - strand, within BBR_RS18720 at 1991.150 kb on - strand, within BBR_RS18720 at 1991.150 kb on - strand, within BBR_RS18720 at 1991.161 kb on - strand, within BBR_RS18720 at 1991.180 kb on + strand, within BBR_RS18720 at 1991.188 kb on + strand, within BBR_RS18720 at 1991.196 kb on - strand, within BBR_RS18720 at 1991.203 kb on + strand, within BBR_RS18720 at 1991.209 kb on - strand, within BBR_RS18720 at 1991.221 kb on - strand, within BBR_RS18720 at 1991.230 kb on + strand, within BBR_RS18720 at 1991.232 kb on + strand, within BBR_RS18720 at 1991.232 kb on - strand, within BBR_RS18720 at 1991.246 kb on + strand, within BBR_RS18720 at 1991.269 kb on - strand, within BBR_RS18720 at 1991.269 kb on - strand, within BBR_RS18720 at 1991.271 kb on - strand, within BBR_RS18720 at 1991.280 kb on + strand, within BBR_RS18720 at 1991.285 kb on + strand, within BBR_RS18720 at 1991.303 kb on - strand, within BBR_RS18720 at 1991.303 kb on - strand, within BBR_RS18720 at 1991.305 kb on + strand, within BBR_RS18720 at 1991.305 kb on + strand, within BBR_RS18720 at 1991.313 kb on - strand, within BBR_RS18720 at 1991.313 kb on - strand, within BBR_RS18720 at 1991.313 kb on - strand, within BBR_RS18720 at 1991.313 kb on - strand, within BBR_RS18720 at 1991.313 kb on - strand, within BBR_RS18720 at 1991.343 kb on + strand, within BBR_RS18720 at 1991.346 kb on - strand, within BBR_RS18720 at 1991.346 kb on - strand, within BBR_RS18720 at 1991.361 kb on - strand, within BBR_RS18720 at 1991.364 kb on + strand, within BBR_RS18720 at 1991.370 kb on - strand, within BBR_RS18720 at 1991.372 kb on - strand, within BBR_RS18720 at 1991.418 kb on - strand, within BBR_RS18720 at 1991.418 kb on - strand, within BBR_RS18720 at 1991.477 kb on + strand, within BBR_RS18720 at 1991.523 kb on + strand at 1991.523 kb on + strand at 1991.523 kb on - strand at 1991.523 kb on - strand at 1991.531 kb on - strand at 1991.531 kb on - strand at 1991.533 kb on - strand at 1991.536 kb on - strand at 1991.565 kb on - strand at 1991.596 kb on + strand at 1991.596 kb on + strand at 1991.599 kb on - strand at 1991.604 kb on - strand at 1991.604 kb on - strand at 1991.627 kb on + strand at 1991.645 kb on + strand at 1991.645 kb on + strand at 1991.653 kb on - strand at 1991.656 kb on + strand at 1991.685 kb on + strand at 1991.702 kb on - strand at 1991.702 kb on - strand at 1991.702 kb on - strand at 1991.712 kb on - strand at 1991.721 kb on - strand at 1992.290 kb on + strand, within BBR_RS18725 at 1992.312 kb on + strand, within BBR_RS18725 at 1992.317 kb on + strand, within BBR_RS18725 at 1992.351 kb on - strand, within BBR_RS18725 at 1992.384 kb on - strand, within BBR_RS18725 at 1992.429 kb on + strand, within BBR_RS18725 at 1992.429 kb on + strand, within BBR_RS18725 at 1992.481 kb on - strand, within BBR_RS18725 at 1992.614 kb on - strand, within BBR_RS18725 at 1992.823 kb on - strand at 1992.884 kb on + strand at 1992.884 kb on + strand at 1992.886 kb on + strand at 1992.891 kb on + strand at 1992.892 kb on + strand at 1992.892 kb on + strand at 1992.892 kb on - strand at 1992.895 kb on - strand at 1992.899 kb on + strand at 1992.900 kb on - strand at 1992.900 kb on - strand at 1992.915 kb on - strand at 1992.915 kb on - strand at 1992.915 kb on - strand at 1993.626 kb on - strand at 1993.636 kb on + strand at 1993.644 kb on + strand at 1993.676 kb on - strand at 1993.710 kb on + strand at 1993.710 kb on - strand at 1993.710 kb on - strand at 1993.718 kb on - strand at 1993.748 kb on - strand at 1993.765 kb on - strand at 1993.766 kb on - strand at 1993.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,990,724 + BBR_RS18720 0.37 -0.2 1,990,727 - BBR_RS18720 0.38 +0.3 1,990,732 - BBR_RS18720 0.38 +0.3 1,990,733 + BBR_RS18720 0.38 +0.9 1,990,770 - BBR_RS18720 0.40 -0.2 1,990,772 + BBR_RS18720 0.41 -0.8 1,990,784 + BBR_RS18720 0.41 +0.6 1,990,784 + BBR_RS18720 0.41 +0.1 1,990,789 + BBR_RS18720 0.42 +0.5 1,990,792 - BBR_RS18720 0.42 +0.0 1,990,792 - BBR_RS18720 0.42 -0.5 1,990,792 - BBR_RS18720 0.42 -0.3 1,990,817 + BBR_RS18720 0.44 +0.1 1,990,847 + BBR_RS18720 0.46 +0.5 1,990,857 - BBR_RS18720 0.46 +0.2 1,990,857 - BBR_RS18720 0.46 +0.3 1,990,863 - BBR_RS18720 0.47 -1.5 1,990,891 - BBR_RS18720 0.48 +1.0 1,990,912 + BBR_RS18720 0.50 -0.3 1,990,933 - BBR_RS18720 0.51 +0.2 1,990,940 + BBR_RS18720 0.52 -0.2 1,990,945 + BBR_RS18720 0.52 +1.4 1,990,957 + BBR_RS18720 0.53 +0.5 1,990,965 - BBR_RS18720 0.53 -0.2 1,990,970 - BBR_RS18720 0.54 -0.7 1,990,995 + BBR_RS18720 0.55 +0.0 1,991,000 + BBR_RS18720 0.56 +0.4 1,991,019 + BBR_RS18720 0.57 +0.3 1,991,022 - BBR_RS18720 0.57 +0.8 1,991,022 - BBR_RS18720 0.57 +0.4 1,991,029 + BBR_RS18720 0.58 +0.0 1,991,032 - BBR_RS18720 0.58 -0.1 1,991,040 - BBR_RS18720 0.58 +0.2 1,991,049 + BBR_RS18720 0.59 +0.9 1,991,049 + BBR_RS18720 0.59 +0.4 1,991,049 - BBR_RS18720 0.59 +0.0 1,991,052 - BBR_RS18720 0.59 +0.3 1,991,060 + BBR_RS18720 0.60 +0.4 1,991,101 - BBR_RS18720 0.62 +0.7 1,991,101 - BBR_RS18720 0.62 +0.0 1,991,121 - BBR_RS18720 0.64 +0.5 1,991,142 + BBR_RS18720 0.65 +0.1 1,991,150 - BBR_RS18720 0.66 -0.5 1,991,150 - BBR_RS18720 0.66 +0.6 1,991,150 - BBR_RS18720 0.66 -1.3 1,991,161 - BBR_RS18720 0.66 +0.5 1,991,180 + BBR_RS18720 0.68 +0.2 1,991,188 + BBR_RS18720 0.68 -1.4 1,991,196 - BBR_RS18720 0.69 +0.4 1,991,203 + BBR_RS18720 0.69 -0.2 1,991,209 - BBR_RS18720 0.70 +0.6 1,991,221 - BBR_RS18720 0.70 -0.3 1,991,230 + BBR_RS18720 0.71 +0.5 1,991,232 + BBR_RS18720 0.71 -0.4 1,991,232 - BBR_RS18720 0.71 -0.3 1,991,246 + BBR_RS18720 0.72 -0.9 1,991,269 - BBR_RS18720 0.73 +0.4 1,991,269 - BBR_RS18720 0.73 +0.7 1,991,271 - BBR_RS18720 0.74 +1.0 1,991,280 + BBR_RS18720 0.74 -0.2 1,991,285 + BBR_RS18720 0.75 -1.3 1,991,303 - BBR_RS18720 0.76 -0.3 1,991,303 - BBR_RS18720 0.76 +0.4 1,991,305 + BBR_RS18720 0.76 +0.2 1,991,305 + BBR_RS18720 0.76 -0.3 1,991,313 - BBR_RS18720 0.76 +0.6 1,991,313 - BBR_RS18720 0.76 -0.3 1,991,313 - BBR_RS18720 0.76 -0.1 1,991,313 - BBR_RS18720 0.76 +0.3 1,991,313 - BBR_RS18720 0.76 +0.5 1,991,343 + BBR_RS18720 0.78 +0.0 1,991,346 - BBR_RS18720 0.79 -0.0 1,991,346 - BBR_RS18720 0.79 +0.4 1,991,361 - BBR_RS18720 0.80 +0.1 1,991,364 + BBR_RS18720 0.80 +0.2 1,991,370 - BBR_RS18720 0.80 -0.3 1,991,372 - BBR_RS18720 0.80 +0.4 1,991,418 - BBR_RS18720 0.83 -0.3 1,991,418 - BBR_RS18720 0.83 +0.0 1,991,477 + BBR_RS18720 0.87 +0.9 1,991,523 + -0.0 1,991,523 + +0.1 1,991,523 - -0.4 1,991,523 - +0.0 1,991,531 - +0.1 1,991,531 - +0.3 1,991,533 - -0.7 1,991,536 - +0.5 1,991,565 - +0.1 1,991,596 + +0.6 1,991,596 + +1.1 1,991,599 - -0.5 1,991,604 - +0.3 1,991,604 - -0.3 1,991,627 + +0.5 1,991,645 + -0.0 1,991,645 + +0.7 1,991,653 - +0.5 1,991,656 + +0.2 1,991,685 + -0.0 1,991,702 - -0.1 1,991,702 - -0.8 1,991,702 - +0.8 1,991,712 - +0.5 1,991,721 - +0.5 1,992,290 + BBR_RS18725 0.52 +0.8 1,992,312 + BBR_RS18725 0.54 -0.9 1,992,317 + BBR_RS18725 0.54 +2.4 1,992,351 - BBR_RS18725 0.57 +1.2 1,992,384 - BBR_RS18725 0.60 -0.7 1,992,429 + BBR_RS18725 0.64 +0.2 1,992,429 + BBR_RS18725 0.64 -0.4 1,992,481 - BBR_RS18725 0.69 -1.2 1,992,614 - BBR_RS18725 0.81 -2.2 1,992,823 - +0.5 1,992,884 + -0.1 1,992,884 + +0.3 1,992,886 + -1.1 1,992,891 + -0.1 1,992,892 + +0.6 1,992,892 + -0.4 1,992,892 - -0.4 1,992,895 - +0.7 1,992,899 + +0.2 1,992,900 - +0.3 1,992,900 - -0.9 1,992,915 - -0.1 1,992,915 - -0.3 1,992,915 - -0.5 1,993,626 - -0.8 1,993,636 + +0.3 1,993,644 + +0.8 1,993,676 - -0.9 1,993,710 + +0.2 1,993,710 - -0.1 1,993,710 - -0.2 1,993,718 - -0.8 1,993,748 - +0.7 1,993,765 - +0.4 1,993,766 - -0.3 1,993,795 - +0.3
Or see this region's nucleotide sequence