Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18690 and BBR_RS18695 are separated by 1 nucleotides BBR_RS18695 and BBR_RS18700 are separated by 51 nucleotides BBR_RS18700 and BBR_RS18705 are separated by 495 nucleotides
BBR_RS18690: BBR_RS18690 - diacylglycerol kinase, at 1,981,209 to 1,982,390
_RS18690
BBR_RS18695: BBR_RS18695 - PRD domain-containing protein, at 1,982,392 to 1,983,285
_RS18695
BBR_RS18700: BBR_RS18700 - PTS beta-glucoside transporter subunit EIIBCA, at 1,983,337 to 1,985,427
_RS18700
BBR_RS18705: BBR_RS18705 - alpha-D-glucose phosphate-specific phosphoglucomutase, at 1,985,923 to 1,987,599
_RS18705
Position (kb)
1983
1984
1985
1986 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1982.452 kb on + strand at 1982.589 kb on - strand, within BBR_RS18695 at 1982.615 kb on + strand, within BBR_RS18695 at 1982.623 kb on - strand, within BBR_RS18695 at 1982.828 kb on + strand, within BBR_RS18695 at 1982.838 kb on + strand, within BBR_RS18695 at 1982.882 kb on + strand, within BBR_RS18695 at 1982.911 kb on - strand, within BBR_RS18695 at 1982.981 kb on - strand, within BBR_RS18695 at 1983.030 kb on + strand, within BBR_RS18695 at 1983.130 kb on + strand, within BBR_RS18695 at 1983.131 kb on - strand, within BBR_RS18695 at 1983.138 kb on - strand, within BBR_RS18695 at 1983.143 kb on - strand, within BBR_RS18695 at 1983.191 kb on + strand, within BBR_RS18695 at 1983.327 kb on - strand at 1983.327 kb on - strand at 1983.335 kb on - strand at 1983.338 kb on - strand at 1983.357 kb on - strand at 1983.418 kb on + strand at 1983.479 kb on + strand at 1983.493 kb on - strand at 1983.523 kb on - strand at 1983.523 kb on - strand at 1983.660 kb on - strand, within BBR_RS18700 at 1983.700 kb on - strand, within BBR_RS18700 at 1983.710 kb on - strand, within BBR_RS18700 at 1983.741 kb on + strand, within BBR_RS18700 at 1983.741 kb on + strand, within BBR_RS18700 at 1983.749 kb on - strand, within BBR_RS18700 at 1983.749 kb on - strand, within BBR_RS18700 at 1983.880 kb on - strand, within BBR_RS18700 at 1983.906 kb on - strand, within BBR_RS18700 at 1984.157 kb on - strand, within BBR_RS18700 at 1984.194 kb on + strand, within BBR_RS18700 at 1984.364 kb on + strand, within BBR_RS18700 at 1984.402 kb on + strand, within BBR_RS18700 at 1984.402 kb on + strand, within BBR_RS18700 at 1984.407 kb on + strand, within BBR_RS18700 at 1984.456 kb on + strand, within BBR_RS18700 at 1984.496 kb on - strand, within BBR_RS18700 at 1984.635 kb on - strand, within BBR_RS18700 at 1984.679 kb on - strand, within BBR_RS18700 at 1984.711 kb on + strand, within BBR_RS18700 at 1984.730 kb on + strand, within BBR_RS18700 at 1984.786 kb on - strand, within BBR_RS18700 at 1984.792 kb on - strand, within BBR_RS18700 at 1984.830 kb on - strand, within BBR_RS18700 at 1984.861 kb on - strand, within BBR_RS18700 at 1984.986 kb on + strand, within BBR_RS18700 at 1984.993 kb on - strand, within BBR_RS18700 at 1985.037 kb on + strand, within BBR_RS18700 at 1985.122 kb on - strand, within BBR_RS18700 at 1985.122 kb on - strand, within BBR_RS18700 at 1985.122 kb on - strand, within BBR_RS18700 at 1985.147 kb on - strand, within BBR_RS18700 at 1985.205 kb on + strand, within BBR_RS18700 at 1985.235 kb on + strand at 1985.249 kb on - strand at 1985.326 kb on - strand at 1985.382 kb on + strand at 1985.425 kb on - strand at 1985.451 kb on + strand at 1985.456 kb on + strand at 1985.456 kb on + strand at 1985.464 kb on - strand at 1985.464 kb on - strand at 1985.464 kb on - strand at 1985.469 kb on - strand at 1985.484 kb on + strand at 1985.484 kb on + strand at 1985.537 kb on + strand at 1985.550 kb on - strand at 1985.564 kb on - strand at 1985.573 kb on - strand at 1985.592 kb on - strand at 1985.618 kb on - strand at 1985.683 kb on - strand at 1985.726 kb on - strand at 1985.795 kb on + strand at 1985.808 kb on + strand at 1985.811 kb on - strand at 1985.839 kb on + strand at 1985.898 kb on + strand at 1985.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,982,452 + -4.1 1,982,589 - BBR_RS18695 0.22 -3.5 1,982,615 + BBR_RS18695 0.25 -1.8 1,982,623 - BBR_RS18695 0.26 -2.6 1,982,828 + BBR_RS18695 0.49 -3.1 1,982,838 + BBR_RS18695 0.50 -3.3 1,982,882 + BBR_RS18695 0.55 -2.6 1,982,911 - BBR_RS18695 0.58 -1.8 1,982,981 - BBR_RS18695 0.66 -1.8 1,983,030 + BBR_RS18695 0.71 -1.3 1,983,130 + BBR_RS18695 0.83 -2.9 1,983,131 - BBR_RS18695 0.83 -1.9 1,983,138 - BBR_RS18695 0.83 -1.2 1,983,143 - BBR_RS18695 0.84 -1.3 1,983,191 + BBR_RS18695 0.89 -3.4 1,983,327 - -0.1 1,983,327 - -0.3 1,983,335 - +0.4 1,983,338 - +0.2 1,983,357 - -0.3 1,983,418 + -2.4 1,983,479 + -5.0 1,983,493 - -0.6 1,983,523 - -1.7 1,983,523 - -1.5 1,983,660 - BBR_RS18700 0.15 -0.9 1,983,700 - BBR_RS18700 0.17 -0.7 1,983,710 - BBR_RS18700 0.18 -0.6 1,983,741 + BBR_RS18700 0.19 -0.9 1,983,741 + BBR_RS18700 0.19 -1.6 1,983,749 - BBR_RS18700 0.20 -0.5 1,983,749 - BBR_RS18700 0.20 -0.7 1,983,880 - BBR_RS18700 0.26 -0.9 1,983,906 - BBR_RS18700 0.27 -0.6 1,984,157 - BBR_RS18700 0.39 -5.0 1,984,194 + BBR_RS18700 0.41 -4.0 1,984,364 + BBR_RS18700 0.49 -3.1 1,984,402 + BBR_RS18700 0.51 -3.9 1,984,402 + BBR_RS18700 0.51 -1.7 1,984,407 + BBR_RS18700 0.51 -4.9 1,984,456 + BBR_RS18700 0.54 -4.9 1,984,496 - BBR_RS18700 0.55 -4.1 1,984,635 - BBR_RS18700 0.62 -3.3 1,984,679 - BBR_RS18700 0.64 -4.5 1,984,711 + BBR_RS18700 0.66 -4.3 1,984,730 + BBR_RS18700 0.67 -3.4 1,984,786 - BBR_RS18700 0.69 -4.4 1,984,792 - BBR_RS18700 0.70 -3.0 1,984,830 - BBR_RS18700 0.71 -0.2 1,984,861 - BBR_RS18700 0.73 -3.5 1,984,986 + BBR_RS18700 0.79 -4.3 1,984,993 - BBR_RS18700 0.79 -4.7 1,985,037 + BBR_RS18700 0.81 -2.8 1,985,122 - BBR_RS18700 0.85 -3.6 1,985,122 - BBR_RS18700 0.85 -4.7 1,985,122 - BBR_RS18700 0.85 -3.9 1,985,147 - BBR_RS18700 0.87 -4.3 1,985,205 + BBR_RS18700 0.89 -3.7 1,985,235 + -4.4 1,985,249 - -4.7 1,985,326 - -5.4 1,985,382 + -1.7 1,985,425 - -0.7 1,985,451 + -1.2 1,985,456 + -0.8 1,985,456 + -1.0 1,985,464 - +0.0 1,985,464 - -0.0 1,985,464 - -0.1 1,985,469 - -1.0 1,985,484 + -2.1 1,985,484 + -4.7 1,985,537 + -5.1 1,985,550 - +0.0 1,985,564 - +0.2 1,985,573 - -0.7 1,985,592 - -1.3 1,985,618 - -0.9 1,985,683 - +0.6 1,985,726 - +0.1 1,985,795 + -0.1 1,985,808 + +0.2 1,985,811 - -0.7 1,985,839 + +0.4 1,985,898 + -0.2 1,985,921 - -0.8
Or see this region's nucleotide sequence