Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18685 and BBR_RS18690 are separated by 239 nucleotides BBR_RS18690 and BBR_RS18695 are separated by 1 nucleotides BBR_RS18695 and BBR_RS18700 are separated by 51 nucleotides
BBR_RS18685: BBR_RS18685 - serine--tRNA ligase, at 1,979,683 to 1,980,969
_RS18685
BBR_RS18690: BBR_RS18690 - diacylglycerol kinase, at 1,981,209 to 1,982,390
_RS18690
BBR_RS18695: BBR_RS18695 - PRD domain-containing protein, at 1,982,392 to 1,983,285
_RS18695
BBR_RS18700: BBR_RS18700 - PTS beta-glucoside transporter subunit EIIBCA, at 1,983,337 to 1,985,427
_RS18700
Position (kb)
1981
1982
1983 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1981.021 kb on + strand at 1981.026 kb on + strand at 1981.029 kb on - strand at 1981.119 kb on + strand at 1981.133 kb on - strand at 1981.133 kb on - strand at 1981.160 kb on - strand at 1981.163 kb on + strand at 1981.184 kb on - strand at 1981.366 kb on - strand, within BBR_RS18690 at 1981.394 kb on - strand, within BBR_RS18690 at 1981.467 kb on + strand, within BBR_RS18690 at 1981.467 kb on + strand, within BBR_RS18690 at 1981.478 kb on + strand, within BBR_RS18690 at 1981.478 kb on + strand, within BBR_RS18690 at 1981.506 kb on + strand, within BBR_RS18690 at 1981.516 kb on + strand, within BBR_RS18690 at 1981.524 kb on - strand, within BBR_RS18690 at 1981.593 kb on + strand, within BBR_RS18690 at 1981.593 kb on + strand, within BBR_RS18690 at 1981.606 kb on - strand, within BBR_RS18690 at 1981.671 kb on + strand, within BBR_RS18690 at 1981.678 kb on - strand, within BBR_RS18690 at 1981.679 kb on - strand, within BBR_RS18690 at 1981.707 kb on + strand, within BBR_RS18690 at 1981.823 kb on + strand, within BBR_RS18690 at 1981.924 kb on + strand, within BBR_RS18690 at 1981.924 kb on + strand, within BBR_RS18690 at 1981.927 kb on + strand, within BBR_RS18690 at 1981.929 kb on + strand, within BBR_RS18690 at 1981.932 kb on - strand, within BBR_RS18690 at 1982.077 kb on + strand, within BBR_RS18690 at 1982.085 kb on - strand, within BBR_RS18690 at 1982.130 kb on - strand, within BBR_RS18690 at 1982.135 kb on - strand, within BBR_RS18690 at 1982.165 kb on + strand, within BBR_RS18690 at 1982.165 kb on + strand, within BBR_RS18690 at 1982.166 kb on + strand, within BBR_RS18690 at 1982.166 kb on + strand, within BBR_RS18690 at 1982.172 kb on + strand, within BBR_RS18690 at 1982.174 kb on - strand, within BBR_RS18690 at 1982.210 kb on + strand, within BBR_RS18690 at 1982.210 kb on + strand, within BBR_RS18690 at 1982.212 kb on - strand, within BBR_RS18690 at 1982.216 kb on + strand, within BBR_RS18690 at 1982.218 kb on - strand, within BBR_RS18690 at 1982.263 kb on - strand, within BBR_RS18690 at 1982.309 kb on + strand at 1982.317 kb on - strand at 1982.317 kb on - strand at 1982.331 kb on + strand at 1982.336 kb on + strand at 1982.452 kb on + strand at 1982.589 kb on - strand, within BBR_RS18695 at 1982.615 kb on + strand, within BBR_RS18695 at 1982.623 kb on - strand, within BBR_RS18695 at 1982.828 kb on + strand, within BBR_RS18695 at 1982.838 kb on + strand, within BBR_RS18695 at 1982.882 kb on + strand, within BBR_RS18695 at 1982.911 kb on - strand, within BBR_RS18695 at 1982.981 kb on - strand, within BBR_RS18695 at 1983.030 kb on + strand, within BBR_RS18695 at 1983.130 kb on + strand, within BBR_RS18695 at 1983.131 kb on - strand, within BBR_RS18695 at 1983.138 kb on - strand, within BBR_RS18695 at 1983.143 kb on - strand, within BBR_RS18695 at 1983.191 kb on + strand, within BBR_RS18695 at 1983.327 kb on - strand at 1983.327 kb on - strand at 1983.335 kb on - strand at 1983.338 kb on - strand at 1983.357 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,981,021 + -0.1 1,981,026 + +0.7 1,981,029 - +0.1 1,981,119 + +0.3 1,981,133 - -0.7 1,981,133 - -0.2 1,981,160 - -1.2 1,981,163 + +0.4 1,981,184 - -0.1 1,981,366 - BBR_RS18690 0.13 +0.8 1,981,394 - BBR_RS18690 0.16 -0.4 1,981,467 + BBR_RS18690 0.22 -0.6 1,981,467 + BBR_RS18690 0.22 -0.0 1,981,478 + BBR_RS18690 0.23 -1.3 1,981,478 + BBR_RS18690 0.23 -2.8 1,981,506 + BBR_RS18690 0.25 -0.0 1,981,516 + BBR_RS18690 0.26 +0.0 1,981,524 - BBR_RS18690 0.27 +0.4 1,981,593 + BBR_RS18690 0.32 +0.6 1,981,593 + BBR_RS18690 0.32 -1.5 1,981,606 - BBR_RS18690 0.34 -2.3 1,981,671 + BBR_RS18690 0.39 -0.6 1,981,678 - BBR_RS18690 0.40 -2.3 1,981,679 - BBR_RS18690 0.40 +1.8 1,981,707 + BBR_RS18690 0.42 -0.7 1,981,823 + BBR_RS18690 0.52 -1.5 1,981,924 + BBR_RS18690 0.60 -2.4 1,981,924 + BBR_RS18690 0.60 +1.4 1,981,927 + BBR_RS18690 0.61 -0.6 1,981,929 + BBR_RS18690 0.61 -1.9 1,981,932 - BBR_RS18690 0.61 +0.3 1,982,077 + BBR_RS18690 0.73 -1.6 1,982,085 - BBR_RS18690 0.74 -0.4 1,982,130 - BBR_RS18690 0.78 -2.1 1,982,135 - BBR_RS18690 0.78 -1.7 1,982,165 + BBR_RS18690 0.81 -0.5 1,982,165 + BBR_RS18690 0.81 +0.6 1,982,166 + BBR_RS18690 0.81 -0.8 1,982,166 + BBR_RS18690 0.81 -0.7 1,982,172 + BBR_RS18690 0.81 -2.0 1,982,174 - BBR_RS18690 0.82 -1.2 1,982,210 + BBR_RS18690 0.85 -2.5 1,982,210 + BBR_RS18690 0.85 -1.0 1,982,212 - BBR_RS18690 0.85 -0.4 1,982,216 + BBR_RS18690 0.85 -0.2 1,982,218 - BBR_RS18690 0.85 -2.6 1,982,263 - BBR_RS18690 0.89 +0.3 1,982,309 + +0.3 1,982,317 - +0.1 1,982,317 - +0.6 1,982,331 + -0.4 1,982,336 + +0.5 1,982,452 + -4.1 1,982,589 - BBR_RS18695 0.22 -3.5 1,982,615 + BBR_RS18695 0.25 -1.8 1,982,623 - BBR_RS18695 0.26 -2.6 1,982,828 + BBR_RS18695 0.49 -3.1 1,982,838 + BBR_RS18695 0.50 -3.3 1,982,882 + BBR_RS18695 0.55 -2.6 1,982,911 - BBR_RS18695 0.58 -1.8 1,982,981 - BBR_RS18695 0.66 -1.8 1,983,030 + BBR_RS18695 0.71 -1.3 1,983,130 + BBR_RS18695 0.83 -2.9 1,983,131 - BBR_RS18695 0.83 -1.9 1,983,138 - BBR_RS18695 0.83 -1.2 1,983,143 - BBR_RS18695 0.84 -1.3 1,983,191 + BBR_RS18695 0.89 -3.4 1,983,327 - -0.1 1,983,327 - -0.3 1,983,335 - +0.4 1,983,338 - +0.2 1,983,357 - -0.3
Or see this region's nucleotide sequence