Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18625 and BBR_RS18630 are separated by 120 nucleotides BBR_RS18630 and BBR_RS18635 overlap by 4 nucleotides BBR_RS18635 and BBR_RS18640 are separated by 128 nucleotides
BBR_RS18625: BBR_RS18625 - alpha/beta hydrolase, at 1,964,241 to 1,965,965
_RS18625
BBR_RS18630: BBR_RS18630 - hypothetical protein, at 1,966,086 to 1,966,805
_RS18630
BBR_RS18635: BBR_RS18635 - PspC domain-containing protein, at 1,966,802 to 1,968,484
_RS18635
BBR_RS18640: BBR_RS18640 - PspC domain-containing protein, at 1,968,613 to 1,969,899
_RS18640
Position (kb)
1966
1967
1968
1969 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1965.841 kb on - strand at 1965.847 kb on + strand at 1965.847 kb on + strand at 1965.855 kb on - strand at 1965.859 kb on + strand at 1965.867 kb on - strand at 1965.891 kb on - strand at 1965.902 kb on - strand at 1965.946 kb on - strand at 1965.981 kb on - strand at 1965.986 kb on + strand at 1965.986 kb on + strand at 1965.987 kb on + strand at 1965.988 kb on + strand at 1965.994 kb on - strand at 1966.017 kb on + strand at 1966.025 kb on - strand at 1966.121 kb on - strand at 1966.835 kb on - strand at 1966.910 kb on - strand at 1966.917 kb on - strand at 1966.936 kb on - strand at 1966.953 kb on - strand at 1967.301 kb on - strand, within BBR_RS18635 at 1967.328 kb on - strand, within BBR_RS18635 at 1967.434 kb on - strand, within BBR_RS18635 at 1967.441 kb on - strand, within BBR_RS18635 at 1967.441 kb on - strand, within BBR_RS18635 at 1967.441 kb on - strand, within BBR_RS18635 at 1967.489 kb on - strand, within BBR_RS18635 at 1967.505 kb on - strand, within BBR_RS18635 at 1967.525 kb on - strand, within BBR_RS18635 at 1967.589 kb on - strand, within BBR_RS18635 at 1967.783 kb on - strand, within BBR_RS18635 at 1967.934 kb on - strand, within BBR_RS18635 at 1968.046 kb on - strand, within BBR_RS18635 at 1968.168 kb on + strand, within BBR_RS18635 at 1968.286 kb on - strand, within BBR_RS18635 at 1968.305 kb on - strand, within BBR_RS18635 at 1968.353 kb on - strand at 1968.377 kb on - strand at 1968.428 kb on - strand at 1968.429 kb on - strand at 1968.492 kb on - strand at 1968.492 kb on - strand at 1968.494 kb on + strand at 1968.504 kb on + strand at 1968.504 kb on + strand at 1968.541 kb on - strand at 1968.607 kb on + strand at 1968.608 kb on - strand at 1968.608 kb on - strand at 1968.611 kb on - strand at 1968.611 kb on - strand at 1968.652 kb on + strand at 1968.660 kb on - strand at 1968.660 kb on - strand at 1968.660 kb on - strand at 1968.663 kb on + strand at 1968.671 kb on - strand at 1968.720 kb on - strand at 1968.742 kb on - strand, within BBR_RS18640 at 1968.754 kb on + strand, within BBR_RS18640 at 1968.762 kb on - strand, within BBR_RS18640 at 1968.762 kb on - strand, within BBR_RS18640 at 1968.762 kb on - strand, within BBR_RS18640 at 1968.762 kb on - strand, within BBR_RS18640 at 1968.772 kb on - strand, within BBR_RS18640 at 1968.772 kb on - strand, within BBR_RS18640 at 1968.781 kb on + strand, within BBR_RS18640 at 1968.857 kb on - strand, within BBR_RS18640 at 1968.857 kb on - strand, within BBR_RS18640 at 1968.874 kb on - strand, within BBR_RS18640 at 1968.890 kb on + strand, within BBR_RS18640 at 1968.898 kb on - strand, within BBR_RS18640 at 1968.908 kb on - strand, within BBR_RS18640 at 1968.908 kb on - strand, within BBR_RS18640 at 1968.933 kb on + strand, within BBR_RS18640 at 1968.987 kb on - strand, within BBR_RS18640 at 1969.002 kb on - strand, within BBR_RS18640 at 1969.069 kb on - strand, within BBR_RS18640 at 1969.079 kb on - strand, within BBR_RS18640 at 1969.081 kb on + strand, within BBR_RS18640 at 1969.120 kb on - strand, within BBR_RS18640 at 1969.120 kb on - strand, within BBR_RS18640 at 1969.172 kb on - strand, within BBR_RS18640 at 1969.174 kb on + strand, within BBR_RS18640 at 1969.208 kb on - strand, within BBR_RS18640 at 1969.313 kb on + strand, within BBR_RS18640 at 1969.428 kb on - strand, within BBR_RS18640 at 1969.428 kb on - strand, within BBR_RS18640 at 1969.428 kb on - strand, within BBR_RS18640
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,965,841 - -0.5 1,965,847 + -0.4 1,965,847 + +1.0 1,965,855 - -1.0 1,965,859 + -0.9 1,965,867 - -0.5 1,965,891 - +1.1 1,965,902 - +0.3 1,965,946 - +0.2 1,965,981 - -0.3 1,965,986 + +0.2 1,965,986 + -0.8 1,965,987 + +0.8 1,965,988 + -0.2 1,965,994 - +0.1 1,966,017 + +0.0 1,966,025 - -1.2 1,966,121 - +0.5 1,966,835 - -1.1 1,966,910 - -0.8 1,966,917 - -1.0 1,966,936 - -0.7 1,966,953 - -2.1 1,967,301 - BBR_RS18635 0.30 -2.2 1,967,328 - BBR_RS18635 0.31 +2.4 1,967,434 - BBR_RS18635 0.38 -1.9 1,967,441 - BBR_RS18635 0.38 -1.2 1,967,441 - BBR_RS18635 0.38 -2.1 1,967,441 - BBR_RS18635 0.38 -1.0 1,967,489 - BBR_RS18635 0.41 -1.7 1,967,505 - BBR_RS18635 0.42 -0.4 1,967,525 - BBR_RS18635 0.43 +0.3 1,967,589 - BBR_RS18635 0.47 -1.5 1,967,783 - BBR_RS18635 0.58 +1.0 1,967,934 - BBR_RS18635 0.67 +0.4 1,968,046 - BBR_RS18635 0.74 -1.4 1,968,168 + BBR_RS18635 0.81 +2.4 1,968,286 - BBR_RS18635 0.88 -0.8 1,968,305 - BBR_RS18635 0.89 -0.2 1,968,353 - -0.7 1,968,377 - -0.8 1,968,428 - -1.1 1,968,429 - -0.4 1,968,492 - +0.2 1,968,492 - -0.4 1,968,494 + -0.4 1,968,504 + -1.0 1,968,504 + +0.8 1,968,541 - -1.1 1,968,607 + -1.0 1,968,608 - +0.1 1,968,608 - +0.2 1,968,611 - +0.2 1,968,611 - -0.0 1,968,652 + +0.5 1,968,660 - +0.5 1,968,660 - +0.1 1,968,660 - -0.2 1,968,663 + +0.1 1,968,671 - +1.4 1,968,720 - +0.5 1,968,742 - BBR_RS18640 0.10 +0.6 1,968,754 + BBR_RS18640 0.11 +0.6 1,968,762 - BBR_RS18640 0.12 +1.4 1,968,762 - BBR_RS18640 0.12 -0.0 1,968,762 - BBR_RS18640 0.12 +1.4 1,968,762 - BBR_RS18640 0.12 +0.2 1,968,772 - BBR_RS18640 0.12 +0.4 1,968,772 - BBR_RS18640 0.12 +0.1 1,968,781 + BBR_RS18640 0.13 -0.2 1,968,857 - BBR_RS18640 0.19 -1.7 1,968,857 - BBR_RS18640 0.19 +1.3 1,968,874 - BBR_RS18640 0.20 +0.8 1,968,890 + BBR_RS18640 0.22 +0.4 1,968,898 - BBR_RS18640 0.22 +1.0 1,968,908 - BBR_RS18640 0.23 +1.0 1,968,908 - BBR_RS18640 0.23 +0.6 1,968,933 + BBR_RS18640 0.25 -1.1 1,968,987 - BBR_RS18640 0.29 +0.5 1,969,002 - BBR_RS18640 0.30 +0.2 1,969,069 - BBR_RS18640 0.35 +1.3 1,969,079 - BBR_RS18640 0.36 +0.2 1,969,081 + BBR_RS18640 0.36 +1.0 1,969,120 - BBR_RS18640 0.39 +0.2 1,969,120 - BBR_RS18640 0.39 -1.4 1,969,172 - BBR_RS18640 0.43 +1.0 1,969,174 + BBR_RS18640 0.44 +0.0 1,969,208 - BBR_RS18640 0.46 +0.4 1,969,313 + BBR_RS18640 0.54 -1.0 1,969,428 - BBR_RS18640 0.63 +1.0 1,969,428 - BBR_RS18640 0.63 -0.4 1,969,428 - BBR_RS18640 0.63 -0.4
Or see this region's nucleotide sequence