Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18210 and BBR_RS18215 overlap by 1 nucleotides BBR_RS18215 and BBR_RS18220 are separated by 77 nucleotides
BBR_RS18210: BBR_RS18210 - N-acetylglucosaminyltransferase, at 1,872,562 to 1,873,785
_RS18210
BBR_RS18215: BBR_RS18215 - AI-2E family transporter, at 1,873,785 to 1,875,377
_RS18215
BBR_RS18220: BBR_RS18220 - glycosyltransferase family 2 protein, at 1,875,455 to 1,876,381
_RS18220
Position (kb)
1873
1874
1875
1876 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1873.791 kb on - strand at 1873.796 kb on - strand at 1873.806 kb on - strand at 1873.817 kb on - strand at 1873.821 kb on + strand at 1873.842 kb on + strand at 1873.842 kb on + strand at 1873.868 kb on + strand at 1873.955 kb on - strand, within BBR_RS18215 at 1873.955 kb on - strand, within BBR_RS18215 at 1873.955 kb on - strand, within BBR_RS18215 at 1873.972 kb on - strand, within BBR_RS18215 at 1873.989 kb on - strand, within BBR_RS18215 at 1873.989 kb on - strand, within BBR_RS18215 at 1874.032 kb on - strand, within BBR_RS18215 at 1874.055 kb on - strand, within BBR_RS18215 at 1874.114 kb on - strand, within BBR_RS18215 at 1874.232 kb on - strand, within BBR_RS18215 at 1874.242 kb on - strand, within BBR_RS18215 at 1874.254 kb on - strand, within BBR_RS18215 at 1874.256 kb on - strand, within BBR_RS18215 at 1874.268 kb on - strand, within BBR_RS18215 at 1874.334 kb on - strand, within BBR_RS18215 at 1874.438 kb on - strand, within BBR_RS18215 at 1874.460 kb on - strand, within BBR_RS18215 at 1874.460 kb on - strand, within BBR_RS18215 at 1874.553 kb on - strand, within BBR_RS18215 at 1874.555 kb on - strand, within BBR_RS18215 at 1874.622 kb on - strand, within BBR_RS18215 at 1874.685 kb on - strand, within BBR_RS18215 at 1874.781 kb on - strand, within BBR_RS18215 at 1874.834 kb on - strand, within BBR_RS18215 at 1874.971 kb on + strand, within BBR_RS18215 at 1874.971 kb on + strand, within BBR_RS18215 at 1874.971 kb on + strand, within BBR_RS18215 at 1874.971 kb on + strand, within BBR_RS18215 at 1874.971 kb on + strand, within BBR_RS18215 at 1874.971 kb on + strand, within BBR_RS18215 at 1874.971 kb on + strand, within BBR_RS18215 at 1874.979 kb on - strand, within BBR_RS18215 at 1874.979 kb on - strand, within BBR_RS18215 at 1874.979 kb on - strand, within BBR_RS18215 at 1874.979 kb on - strand, within BBR_RS18215 at 1875.051 kb on - strand, within BBR_RS18215 at 1875.065 kb on - strand, within BBR_RS18215 at 1875.106 kb on + strand, within BBR_RS18215 at 1875.124 kb on - strand, within BBR_RS18215 at 1875.184 kb on - strand, within BBR_RS18215 at 1875.231 kb on - strand at 1875.236 kb on - strand at 1875.236 kb on - strand at 1875.279 kb on - strand at 1875.279 kb on - strand at 1875.279 kb on - strand at 1875.288 kb on - strand at 1875.309 kb on - strand at 1875.389 kb on + strand at 1875.392 kb on - strand at 1875.399 kb on + strand at 1875.417 kb on + strand at 1875.425 kb on + strand at 1875.425 kb on + strand at 1875.425 kb on + strand at 1875.425 kb on + strand at 1875.446 kb on + strand at 1875.493 kb on + strand at 1875.500 kb on - strand at 1875.523 kb on - strand at 1875.525 kb on - strand at 1875.529 kb on + strand at 1875.533 kb on - strand at 1875.545 kb on - strand at 1875.547 kb on - strand at 1875.547 kb on - strand at 1875.547 kb on - strand at 1875.547 kb on - strand at 1875.547 kb on - strand at 1875.565 kb on + strand, within BBR_RS18220 at 1875.589 kb on - strand, within BBR_RS18220 at 1875.604 kb on - strand, within BBR_RS18220 at 1875.604 kb on - strand, within BBR_RS18220 at 1875.605 kb on - strand, within BBR_RS18220 at 1875.628 kb on - strand, within BBR_RS18220 at 1875.642 kb on - strand, within BBR_RS18220 at 1875.649 kb on - strand, within BBR_RS18220 at 1875.692 kb on - strand, within BBR_RS18220 at 1875.715 kb on + strand, within BBR_RS18220 at 1875.798 kb on - strand, within BBR_RS18220 at 1875.809 kb on + strand, within BBR_RS18220 at 1875.815 kb on - strand, within BBR_RS18220 at 1875.818 kb on + strand, within BBR_RS18220 at 1875.841 kb on - strand, within BBR_RS18220 at 1875.844 kb on - strand, within BBR_RS18220 at 1875.846 kb on + strand, within BBR_RS18220 at 1875.846 kb on + strand, within BBR_RS18220 at 1875.867 kb on + strand, within BBR_RS18220 at 1875.925 kb on + strand, within BBR_RS18220 at 1875.932 kb on + strand, within BBR_RS18220 at 1875.971 kb on - strand, within BBR_RS18220 at 1875.979 kb on + strand, within BBR_RS18220 at 1876.022 kb on + strand, within BBR_RS18220 at 1876.022 kb on + strand, within BBR_RS18220 at 1876.037 kb on + strand, within BBR_RS18220 at 1876.038 kb on - strand, within BBR_RS18220 at 1876.046 kb on + strand, within BBR_RS18220 at 1876.055 kb on - strand, within BBR_RS18220 at 1876.102 kb on + strand, within BBR_RS18220 at 1876.107 kb on + strand, within BBR_RS18220 at 1876.151 kb on - strand, within BBR_RS18220 at 1876.157 kb on + strand, within BBR_RS18220 at 1876.182 kb on - strand, within BBR_RS18220 at 1876.212 kb on - strand, within BBR_RS18220 at 1876.212 kb on - strand, within BBR_RS18220 at 1876.217 kb on + strand, within BBR_RS18220 at 1876.217 kb on + strand, within BBR_RS18220 at 1876.222 kb on + strand, within BBR_RS18220 at 1876.222 kb on + strand, within BBR_RS18220 at 1876.222 kb on + strand, within BBR_RS18220 at 1876.227 kb on + strand, within BBR_RS18220 at 1876.230 kb on - strand, within BBR_RS18220 at 1876.230 kb on - strand, within BBR_RS18220 at 1876.230 kb on - strand, within BBR_RS18220 at 1876.235 kb on - strand, within BBR_RS18220 at 1876.244 kb on + strand, within BBR_RS18220 at 1876.246 kb on + strand, within BBR_RS18220 at 1876.252 kb on - strand, within BBR_RS18220 at 1876.256 kb on + strand, within BBR_RS18220 at 1876.262 kb on - strand, within BBR_RS18220 at 1876.262 kb on - strand, within BBR_RS18220 at 1876.270 kb on + strand, within BBR_RS18220 at 1876.314 kb on - strand at 1876.314 kb on - strand at 1876.327 kb on + strand at 1876.327 kb on + strand at 1876.327 kb on + strand at 1876.335 kb on - strand at 1876.335 kb on - strand at 1876.349 kb on + strand at 1876.354 kb on + strand at 1876.362 kb on - strand at 1876.363 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,873,791 - +0.3 1,873,796 - +0.3 1,873,806 - -0.0 1,873,817 - -0.4 1,873,821 + -1.3 1,873,842 + -0.5 1,873,842 + -1.9 1,873,868 + +0.3 1,873,955 - BBR_RS18215 0.11 -2.3 1,873,955 - BBR_RS18215 0.11 -2.5 1,873,955 - BBR_RS18215 0.11 -0.8 1,873,972 - BBR_RS18215 0.12 -1.5 1,873,989 - BBR_RS18215 0.13 -1.0 1,873,989 - BBR_RS18215 0.13 +0.5 1,874,032 - BBR_RS18215 0.16 -1.3 1,874,055 - BBR_RS18215 0.17 -2.0 1,874,114 - BBR_RS18215 0.21 +0.1 1,874,232 - BBR_RS18215 0.28 -0.3 1,874,242 - BBR_RS18215 0.29 +0.1 1,874,254 - BBR_RS18215 0.29 -1.0 1,874,256 - BBR_RS18215 0.30 -1.7 1,874,268 - BBR_RS18215 0.30 -0.7 1,874,334 - BBR_RS18215 0.34 -0.9 1,874,438 - BBR_RS18215 0.41 -1.3 1,874,460 - BBR_RS18215 0.42 -1.8 1,874,460 - BBR_RS18215 0.42 +0.1 1,874,553 - BBR_RS18215 0.48 +0.1 1,874,555 - BBR_RS18215 0.48 -2.1 1,874,622 - BBR_RS18215 0.53 -3.3 1,874,685 - BBR_RS18215 0.56 -0.9 1,874,781 - BBR_RS18215 0.63 -1.2 1,874,834 - BBR_RS18215 0.66 -1.1 1,874,971 + BBR_RS18215 0.74 -1.6 1,874,971 + BBR_RS18215 0.74 -1.2 1,874,971 + BBR_RS18215 0.74 -1.5 1,874,971 + BBR_RS18215 0.74 +0.8 1,874,971 + BBR_RS18215 0.74 -2.5 1,874,971 + BBR_RS18215 0.74 -0.9 1,874,971 + BBR_RS18215 0.74 -2.1 1,874,979 - BBR_RS18215 0.75 -0.2 1,874,979 - BBR_RS18215 0.75 -0.0 1,874,979 - BBR_RS18215 0.75 +0.8 1,874,979 - BBR_RS18215 0.75 -0.2 1,875,051 - BBR_RS18215 0.79 -0.4 1,875,065 - BBR_RS18215 0.80 -0.8 1,875,106 + BBR_RS18215 0.83 +0.2 1,875,124 - BBR_RS18215 0.84 -1.7 1,875,184 - BBR_RS18215 0.88 -1.7 1,875,231 - +0.1 1,875,236 - -1.5 1,875,236 - -1.7 1,875,279 - +0.0 1,875,279 - -0.9 1,875,279 - -1.8 1,875,288 - -0.2 1,875,309 - -0.4 1,875,389 + +0.2 1,875,392 - +1.0 1,875,399 + +0.0 1,875,417 + -0.5 1,875,425 + -0.2 1,875,425 + +0.8 1,875,425 + +0.9 1,875,425 + -0.5 1,875,446 + -1.8 1,875,493 + -3.1 1,875,500 - -0.5 1,875,523 - -0.7 1,875,525 - -1.9 1,875,529 + -1.9 1,875,533 - -1.9 1,875,545 - -2.2 1,875,547 - -1.1 1,875,547 - -1.6 1,875,547 - -0.4 1,875,547 - -1.2 1,875,547 - -1.0 1,875,565 + BBR_RS18220 0.12 -1.2 1,875,589 - BBR_RS18220 0.14 -2.7 1,875,604 - BBR_RS18220 0.16 -1.5 1,875,604 - BBR_RS18220 0.16 -2.7 1,875,605 - BBR_RS18220 0.16 -1.6 1,875,628 - BBR_RS18220 0.19 -1.1 1,875,642 - BBR_RS18220 0.20 -1.0 1,875,649 - BBR_RS18220 0.21 -1.5 1,875,692 - BBR_RS18220 0.26 -2.6 1,875,715 + BBR_RS18220 0.28 -0.6 1,875,798 - BBR_RS18220 0.37 -1.5 1,875,809 + BBR_RS18220 0.38 -1.8 1,875,815 - BBR_RS18220 0.39 -0.9 1,875,818 + BBR_RS18220 0.39 -2.2 1,875,841 - BBR_RS18220 0.42 -0.3 1,875,844 - BBR_RS18220 0.42 -1.3 1,875,846 + BBR_RS18220 0.42 -1.6 1,875,846 + BBR_RS18220 0.42 -1.5 1,875,867 + BBR_RS18220 0.44 -0.8 1,875,925 + BBR_RS18220 0.51 -2.2 1,875,932 + BBR_RS18220 0.51 -1.1 1,875,971 - BBR_RS18220 0.56 -0.5 1,875,979 + BBR_RS18220 0.57 -0.9 1,876,022 + BBR_RS18220 0.61 -1.3 1,876,022 + BBR_RS18220 0.61 -1.4 1,876,037 + BBR_RS18220 0.63 -0.7 1,876,038 - BBR_RS18220 0.63 -2.2 1,876,046 + BBR_RS18220 0.64 -0.7 1,876,055 - BBR_RS18220 0.65 -0.9 1,876,102 + BBR_RS18220 0.70 -1.2 1,876,107 + BBR_RS18220 0.70 -1.2 1,876,151 - BBR_RS18220 0.75 -1.2 1,876,157 + BBR_RS18220 0.76 -1.5 1,876,182 - BBR_RS18220 0.78 -2.7 1,876,212 - BBR_RS18220 0.82 +0.1 1,876,212 - BBR_RS18220 0.82 -2.7 1,876,217 + BBR_RS18220 0.82 -2.4 1,876,217 + BBR_RS18220 0.82 -2.6 1,876,222 + BBR_RS18220 0.83 -1.1 1,876,222 + BBR_RS18220 0.83 -2.0 1,876,222 + BBR_RS18220 0.83 -2.4 1,876,227 + BBR_RS18220 0.83 -1.4 1,876,230 - BBR_RS18220 0.84 -1.9 1,876,230 - BBR_RS18220 0.84 +0.3 1,876,230 - BBR_RS18220 0.84 -2.1 1,876,235 - BBR_RS18220 0.84 -0.6 1,876,244 + BBR_RS18220 0.85 -0.7 1,876,246 + BBR_RS18220 0.85 -1.1 1,876,252 - BBR_RS18220 0.86 -2.5 1,876,256 + BBR_RS18220 0.86 -0.2 1,876,262 - BBR_RS18220 0.87 -1.9 1,876,262 - BBR_RS18220 0.87 -2.1 1,876,270 + BBR_RS18220 0.88 -1.5 1,876,314 - -0.1 1,876,314 - -0.2 1,876,327 + +0.9 1,876,327 + -0.3 1,876,327 + +0.6 1,876,335 - -1.1 1,876,335 - -0.5 1,876,349 + +0.7 1,876,354 + -0.4 1,876,362 - -1.0 1,876,363 - -1.1
Or see this region's nucleotide sequence