Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18170 and BBR_RS18175 are separated by 197 nucleotides BBR_RS18175 and BBR_RS18180 are separated by 135 nucleotides BBR_RS18180 and BBR_RS18185 overlap by 4 nucleotides BBR_RS18185 and BBR_RS18190 overlap by 14 nucleotides
BBR_RS18170: BBR_RS18170 - dihydropteroate synthase, at 1,862,194 to 1,863,069
_RS18170
BBR_RS18175: BBR_RS18175 - GTP cyclohydrolase I FolE, at 1,863,267 to 1,863,887
_RS18175
BBR_RS18180: BBR_RS18180 - ATP-dependent zinc metalloprotease FtsH, at 1,864,023 to 1,866,122
_RS18180
BBR_RS18185: BBR_RS18185 - hypoxanthine phosphoribosyltransferase, at 1,866,119 to 1,866,682
_RS18185
BBR_RS18190: BBR_RS18190 - tRNA lysidine(34) synthetase TilS, at 1,866,669 to 1,867,820
_RS18190
Position (kb)
1864
1865
1866
1867 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1863.093 kb on + strand at 1863.098 kb on - strand at 1863.108 kb on + strand at 1863.116 kb on - strand at 1863.116 kb on - strand at 1863.116 kb on - strand at 1863.122 kb on - strand at 1863.126 kb on - strand at 1863.127 kb on - strand at 1863.198 kb on + strand at 1863.211 kb on - strand at 1863.211 kb on - strand at 1863.220 kb on + strand at 1863.228 kb on - strand at 1863.236 kb on - strand at 1863.251 kb on + strand at 1863.310 kb on + strand at 1863.464 kb on - strand, within BBR_RS18175 at 1863.489 kb on - strand, within BBR_RS18175 at 1863.975 kb on + strand at 1864.012 kb on - strand at 1864.068 kb on + strand at 1864.090 kb on - strand at 1864.118 kb on + strand at 1864.136 kb on - strand at 1864.136 kb on - strand at 1864.146 kb on - strand at 1864.163 kb on - strand at 1864.194 kb on - strand at 1864.286 kb on - strand, within BBR_RS18180 at 1864.339 kb on - strand, within BBR_RS18180 at 1864.339 kb on - strand, within BBR_RS18180 at 1864.339 kb on - strand, within BBR_RS18180 at 1864.361 kb on - strand, within BBR_RS18180 at 1864.372 kb on + strand, within BBR_RS18180 at 1864.380 kb on - strand, within BBR_RS18180 at 1864.499 kb on - strand, within BBR_RS18180 at 1864.499 kb on - strand, within BBR_RS18180 at 1864.570 kb on - strand, within BBR_RS18180 at 1864.575 kb on - strand, within BBR_RS18180 at 1864.593 kb on - strand, within BBR_RS18180 at 1864.593 kb on - strand, within BBR_RS18180 at 1864.647 kb on - strand, within BBR_RS18180 at 1864.667 kb on - strand, within BBR_RS18180 at 1864.683 kb on - strand, within BBR_RS18180 at 1864.683 kb on - strand, within BBR_RS18180 at 1864.731 kb on - strand, within BBR_RS18180 at 1864.771 kb on - strand, within BBR_RS18180 at 1864.795 kb on - strand, within BBR_RS18180 at 1864.817 kb on - strand, within BBR_RS18180 at 1864.850 kb on - strand, within BBR_RS18180 at 1864.856 kb on + strand, within BBR_RS18180 at 1864.931 kb on - strand, within BBR_RS18180 at 1864.945 kb on - strand, within BBR_RS18180 at 1864.945 kb on - strand, within BBR_RS18180 at 1864.945 kb on - strand, within BBR_RS18180 at 1864.965 kb on - strand, within BBR_RS18180 at 1865.059 kb on - strand, within BBR_RS18180 at 1865.122 kb on - strand, within BBR_RS18180 at 1865.236 kb on - strand, within BBR_RS18180 at 1865.318 kb on - strand, within BBR_RS18180 at 1865.342 kb on - strand, within BBR_RS18180 at 1865.352 kb on - strand, within BBR_RS18180 at 1865.451 kb on - strand, within BBR_RS18180 at 1865.471 kb on + strand, within BBR_RS18180 at 1865.509 kb on - strand, within BBR_RS18180 at 1865.614 kb on - strand, within BBR_RS18180 at 1865.614 kb on - strand, within BBR_RS18180 at 1865.655 kb on - strand, within BBR_RS18180 at 1865.655 kb on - strand, within BBR_RS18180 at 1865.697 kb on - strand, within BBR_RS18180 at 1865.699 kb on + strand, within BBR_RS18180 at 1865.707 kb on - strand, within BBR_RS18180 at 1865.722 kb on + strand, within BBR_RS18180 at 1865.785 kb on - strand, within BBR_RS18180 at 1865.853 kb on - strand, within BBR_RS18180 at 1865.862 kb on - strand, within BBR_RS18180 at 1865.988 kb on - strand at 1866.010 kb on + strand at 1866.155 kb on - strand at 1866.156 kb on - strand at 1866.173 kb on - strand at 1866.173 kb on - strand at 1866.238 kb on - strand, within BBR_RS18185 at 1866.306 kb on - strand, within BBR_RS18185 at 1866.306 kb on - strand, within BBR_RS18185 at 1866.381 kb on - strand, within BBR_RS18185 at 1866.447 kb on + strand, within BBR_RS18185 at 1866.448 kb on - strand, within BBR_RS18185 at 1866.470 kb on - strand, within BBR_RS18185 at 1866.498 kb on - strand, within BBR_RS18185 at 1866.501 kb on + strand, within BBR_RS18185 at 1866.503 kb on - strand, within BBR_RS18185 at 1866.509 kb on - strand, within BBR_RS18185 at 1866.518 kb on - strand, within BBR_RS18185 at 1866.585 kb on + strand, within BBR_RS18185 at 1866.585 kb on + strand, within BBR_RS18185 at 1866.588 kb on - strand, within BBR_RS18185 at 1866.631 kb on - strand at 1866.636 kb on - strand at 1866.641 kb on - strand at 1866.656 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,863,093 + -0.4 1,863,098 - +0.2 1,863,108 + +0.2 1,863,116 - -0.2 1,863,116 - -0.3 1,863,116 - +0.5 1,863,122 - +0.7 1,863,126 - +0.0 1,863,127 - -0.3 1,863,198 + +0.5 1,863,211 - -0.4 1,863,211 - +0.1 1,863,220 + +0.6 1,863,228 - -0.8 1,863,236 - -0.8 1,863,251 + +0.5 1,863,310 + +1.3 1,863,464 - BBR_RS18175 0.32 +0.7 1,863,489 - BBR_RS18175 0.36 +0.4 1,863,975 + -0.7 1,864,012 - +0.4 1,864,068 + -1.8 1,864,090 - -0.1 1,864,118 + -0.8 1,864,136 - -1.2 1,864,136 - +0.0 1,864,146 - -0.7 1,864,163 - -0.9 1,864,194 - -0.7 1,864,286 - BBR_RS18180 0.13 -1.3 1,864,339 - BBR_RS18180 0.15 -0.8 1,864,339 - BBR_RS18180 0.15 -1.3 1,864,339 - BBR_RS18180 0.15 -1.0 1,864,361 - BBR_RS18180 0.16 -1.1 1,864,372 + BBR_RS18180 0.17 -0.3 1,864,380 - BBR_RS18180 0.17 -1.4 1,864,499 - BBR_RS18180 0.23 -1.1 1,864,499 - BBR_RS18180 0.23 -1.3 1,864,570 - BBR_RS18180 0.26 -0.8 1,864,575 - BBR_RS18180 0.26 -1.3 1,864,593 - BBR_RS18180 0.27 -0.6 1,864,593 - BBR_RS18180 0.27 -2.5 1,864,647 - BBR_RS18180 0.30 -0.6 1,864,667 - BBR_RS18180 0.31 -0.6 1,864,683 - BBR_RS18180 0.31 -1.0 1,864,683 - BBR_RS18180 0.31 -0.7 1,864,731 - BBR_RS18180 0.34 -2.3 1,864,771 - BBR_RS18180 0.36 -0.8 1,864,795 - BBR_RS18180 0.37 -1.2 1,864,817 - BBR_RS18180 0.38 -1.2 1,864,850 - BBR_RS18180 0.39 -1.3 1,864,856 + BBR_RS18180 0.40 -0.7 1,864,931 - BBR_RS18180 0.43 -0.4 1,864,945 - BBR_RS18180 0.44 +0.3 1,864,945 - BBR_RS18180 0.44 +0.0 1,864,945 - BBR_RS18180 0.44 -1.4 1,864,965 - BBR_RS18180 0.45 -1.7 1,865,059 - BBR_RS18180 0.49 -1.6 1,865,122 - BBR_RS18180 0.52 -1.6 1,865,236 - BBR_RS18180 0.58 -1.1 1,865,318 - BBR_RS18180 0.62 +0.3 1,865,342 - BBR_RS18180 0.63 +0.8 1,865,352 - BBR_RS18180 0.63 -2.1 1,865,451 - BBR_RS18180 0.68 -2.7 1,865,471 + BBR_RS18180 0.69 -0.5 1,865,509 - BBR_RS18180 0.71 -2.0 1,865,614 - BBR_RS18180 0.76 -1.4 1,865,614 - BBR_RS18180 0.76 +1.3 1,865,655 - BBR_RS18180 0.78 -2.0 1,865,655 - BBR_RS18180 0.78 -0.5 1,865,697 - BBR_RS18180 0.80 -1.5 1,865,699 + BBR_RS18180 0.80 +1.0 1,865,707 - BBR_RS18180 0.80 -1.2 1,865,722 + BBR_RS18180 0.81 -1.6 1,865,785 - BBR_RS18180 0.84 -0.1 1,865,853 - BBR_RS18180 0.87 -2.0 1,865,862 - BBR_RS18180 0.88 -2.0 1,865,988 - -1.7 1,866,010 + +1.0 1,866,155 - -0.1 1,866,156 - -0.9 1,866,173 - -0.4 1,866,173 - -0.7 1,866,238 - BBR_RS18185 0.21 -0.2 1,866,306 - BBR_RS18185 0.33 +0.2 1,866,306 - BBR_RS18185 0.33 -1.4 1,866,381 - BBR_RS18185 0.46 +1.3 1,866,447 + BBR_RS18185 0.58 +1.1 1,866,448 - BBR_RS18185 0.58 -0.0 1,866,470 - BBR_RS18185 0.62 -0.2 1,866,498 - BBR_RS18185 0.67 -0.0 1,866,501 + BBR_RS18185 0.68 +1.2 1,866,503 - BBR_RS18185 0.68 -1.8 1,866,509 - BBR_RS18185 0.69 +0.1 1,866,518 - BBR_RS18185 0.71 +2.2 1,866,585 + BBR_RS18185 0.83 -2.1 1,866,585 + BBR_RS18185 0.83 +0.3 1,866,588 - BBR_RS18185 0.83 +0.2 1,866,631 - +0.9 1,866,636 - +0.7 1,866,641 - +0.3 1,866,656 + +0.4
Or see this region's nucleotide sequence