Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18165 and BBR_RS18170 are separated by 116 nucleotides BBR_RS18170 and BBR_RS18175 are separated by 197 nucleotides BBR_RS18175 and BBR_RS18180 are separated by 135 nucleotides
BBR_RS18165: BBR_RS18165 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,860,467 to 1,862,077
_RS18165
BBR_RS18170: BBR_RS18170 - dihydropteroate synthase, at 1,862,194 to 1,863,069
_RS18170
BBR_RS18175: BBR_RS18175 - GTP cyclohydrolase I FolE, at 1,863,267 to 1,863,887
_RS18175
BBR_RS18180: BBR_RS18180 - ATP-dependent zinc metalloprotease FtsH, at 1,864,023 to 1,866,122
_RS18180
Position (kb)
1862
1863
1864 Strain fitness (log2 ratio)
-2
-1
0
1 at 1861.244 kb on + strand, within BBR_RS18165 at 1862.125 kb on - strand at 1862.150 kb on - strand at 1862.150 kb on - strand at 1862.247 kb on - strand at 1862.333 kb on - strand, within BBR_RS18170 at 1862.371 kb on - strand, within BBR_RS18170 at 1862.371 kb on - strand, within BBR_RS18170 at 1862.514 kb on + strand, within BBR_RS18170 at 1862.810 kb on - strand, within BBR_RS18170 at 1862.970 kb on - strand, within BBR_RS18170 at 1863.093 kb on + strand at 1863.098 kb on - strand at 1863.108 kb on + strand at 1863.116 kb on - strand at 1863.116 kb on - strand at 1863.116 kb on - strand at 1863.122 kb on - strand at 1863.126 kb on - strand at 1863.127 kb on - strand at 1863.198 kb on + strand at 1863.211 kb on - strand at 1863.211 kb on - strand at 1863.220 kb on + strand at 1863.228 kb on - strand at 1863.236 kb on - strand at 1863.251 kb on + strand at 1863.310 kb on + strand at 1863.464 kb on - strand, within BBR_RS18175 at 1863.489 kb on - strand, within BBR_RS18175 at 1863.975 kb on + strand at 1864.012 kb on - strand at 1864.068 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,861,244 + BBR_RS18165 0.48 +0.3 1,862,125 - -0.5 1,862,150 - +0.2 1,862,150 - -0.1 1,862,247 - -1.5 1,862,333 - BBR_RS18170 0.16 -0.1 1,862,371 - BBR_RS18170 0.20 -0.5 1,862,371 - BBR_RS18170 0.20 +0.2 1,862,514 + BBR_RS18170 0.37 +0.6 1,862,810 - BBR_RS18170 0.70 -1.6 1,862,970 - BBR_RS18170 0.89 -0.5 1,863,093 + -0.4 1,863,098 - +0.2 1,863,108 + +0.2 1,863,116 - -0.2 1,863,116 - -0.3 1,863,116 - +0.5 1,863,122 - +0.7 1,863,126 - +0.0 1,863,127 - -0.3 1,863,198 + +0.5 1,863,211 - -0.4 1,863,211 - +0.1 1,863,220 + +0.6 1,863,228 - -0.8 1,863,236 - -0.8 1,863,251 + +0.5 1,863,310 + +1.3 1,863,464 - BBR_RS18175 0.32 +0.7 1,863,489 - BBR_RS18175 0.36 +0.4 1,863,975 + -0.7 1,864,012 - +0.4 1,864,068 + -1.8
Or see this region's nucleotide sequence