Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18040 and BBR_RS18045 are separated by 88 nucleotides BBR_RS18045 and BBR_RS18050 overlap by 4 nucleotides BBR_RS18050 and BBR_RS18055 are separated by 134 nucleotides
BBR_RS18040: BBR_RS18040 - IMP dehydrogenase, at 1,837,161 to 1,838,714
_RS18040
BBR_RS18045: BBR_RS18045 - undecaprenyl/decaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase, at 1,838,803 to 1,840,050
_RS18045
BBR_RS18050: BBR_RS18050 - threonylcarbamoyl-AMP synthase, at 1,840,047 to 1,840,733
_RS18050
BBR_RS18055: BBR_RS18055 - ABC transporter ATP-binding protein, at 1,840,868 to 1,841,572
_RS18055
Position (kb)
1838
1839
1840
1841 Strain fitness (log2 ratio)
-2
-1
0
1 at 1838.747 kb on + strand at 1838.764 kb on - strand at 1838.764 kb on - strand at 1838.807 kb on - strand at 1838.818 kb on - strand at 1838.818 kb on - strand at 1838.882 kb on - strand at 1838.987 kb on - strand, within BBR_RS18045 at 1840.061 kb on - strand at 1840.074 kb on - strand at 1840.082 kb on - strand at 1840.146 kb on - strand, within BBR_RS18050 at 1840.173 kb on - strand, within BBR_RS18050 at 1840.293 kb on - strand, within BBR_RS18050 at 1840.326 kb on - strand, within BBR_RS18050 at 1840.489 kb on - strand, within BBR_RS18050 at 1840.542 kb on - strand, within BBR_RS18050 at 1840.542 kb on - strand, within BBR_RS18050 at 1840.634 kb on - strand, within BBR_RS18050 at 1840.707 kb on + strand at 1840.717 kb on + strand at 1840.725 kb on - strand at 1840.771 kb on - strand at 1840.795 kb on - strand at 1840.801 kb on + strand at 1840.801 kb on + strand at 1840.801 kb on + strand at 1840.809 kb on - strand at 1840.809 kb on - strand at 1840.851 kb on - strand at 1840.853 kb on + strand at 1840.861 kb on - strand at 1840.861 kb on - strand at 1840.877 kb on + strand at 1840.879 kb on + strand at 1840.927 kb on + strand at 1840.964 kb on - strand, within BBR_RS18055 at 1840.979 kb on - strand, within BBR_RS18055 at 1840.984 kb on - strand, within BBR_RS18055 at 1840.984 kb on - strand, within BBR_RS18055 at 1841.005 kb on - strand, within BBR_RS18055
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,838,747 + -0.7 1,838,764 - -0.0 1,838,764 - -1.0 1,838,807 - +0.3 1,838,818 - -2.5 1,838,818 - -0.2 1,838,882 - -0.5 1,838,987 - BBR_RS18045 0.15 -0.6 1,840,061 - -0.2 1,840,074 - +0.1 1,840,082 - +0.6 1,840,146 - BBR_RS18050 0.14 -1.3 1,840,173 - BBR_RS18050 0.18 -1.3 1,840,293 - BBR_RS18050 0.36 -1.4 1,840,326 - BBR_RS18050 0.41 -0.1 1,840,489 - BBR_RS18050 0.64 -0.0 1,840,542 - BBR_RS18050 0.72 +0.3 1,840,542 - BBR_RS18050 0.72 -1.3 1,840,634 - BBR_RS18050 0.85 +0.6 1,840,707 + -0.2 1,840,717 + -0.1 1,840,725 - -0.1 1,840,771 - +1.0 1,840,795 - +0.4 1,840,801 + -0.8 1,840,801 + -0.8 1,840,801 + -0.3 1,840,809 - +0.6 1,840,809 - -1.0 1,840,851 - +0.0 1,840,853 + -0.3 1,840,861 - +0.1 1,840,861 - -0.4 1,840,877 + -0.8 1,840,879 + -0.3 1,840,927 + +0.9 1,840,964 - BBR_RS18055 0.14 -0.0 1,840,979 - BBR_RS18055 0.16 -0.1 1,840,984 - BBR_RS18055 0.16 +0.2 1,840,984 - BBR_RS18055 0.16 -1.1 1,841,005 - BBR_RS18055 0.19 -0.5
Or see this region's nucleotide sequence