Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17635 and BBR_RS17640 are separated by 166 nucleotides BBR_RS17640 and BBR_RS17645 are separated by 285 nucleotides
BBR_RS17635: BBR_RS17635 - dihydroxy-acid dehydratase, at 1,734,196 to 1,736,058
_RS17635
BBR_RS17640: BBR_RS17640 - DNA-directed RNA polymerase subunit omega, at 1,736,225 to 1,736,509
_RS17640
BBR_RS17645: BBR_RS17645 - methionine adenosyltransferase, at 1,736,795 to 1,738,015
_RS17645
Position (kb)
1736
1737 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1735.225 kb on + strand, within BBR_RS17635 at 1735.226 kb on + strand, within BBR_RS17635 at 1735.226 kb on + strand, within BBR_RS17635 at 1735.233 kb on - strand, within BBR_RS17635 at 1735.233 kb on - strand, within BBR_RS17635 at 1735.234 kb on - strand, within BBR_RS17635 at 1735.234 kb on - strand, within BBR_RS17635 at 1735.234 kb on - strand, within BBR_RS17635 at 1735.236 kb on + strand, within BBR_RS17635 at 1735.244 kb on - strand, within BBR_RS17635 at 1735.244 kb on - strand, within BBR_RS17635 at 1735.244 kb on - strand, within BBR_RS17635 at 1735.252 kb on - strand, within BBR_RS17635 at 1735.307 kb on - strand, within BBR_RS17635 at 1735.321 kb on - strand, within BBR_RS17635 at 1735.360 kb on - strand, within BBR_RS17635 at 1735.360 kb on - strand, within BBR_RS17635 at 1735.369 kb on - strand, within BBR_RS17635 at 1735.380 kb on - strand, within BBR_RS17635 at 1735.382 kb on - strand, within BBR_RS17635 at 1735.397 kb on - strand, within BBR_RS17635 at 1735.410 kb on + strand, within BBR_RS17635 at 1735.418 kb on - strand, within BBR_RS17635 at 1735.418 kb on - strand, within BBR_RS17635 at 1735.456 kb on + strand, within BBR_RS17635 at 1735.456 kb on + strand, within BBR_RS17635 at 1735.464 kb on - strand, within BBR_RS17635 at 1735.473 kb on + strand, within BBR_RS17635 at 1735.504 kb on + strand, within BBR_RS17635 at 1735.546 kb on + strand, within BBR_RS17635 at 1735.549 kb on - strand, within BBR_RS17635 at 1735.552 kb on - strand, within BBR_RS17635 at 1735.557 kb on - strand, within BBR_RS17635 at 1735.563 kb on + strand, within BBR_RS17635 at 1735.570 kb on + strand, within BBR_RS17635 at 1735.571 kb on - strand, within BBR_RS17635 at 1735.578 kb on - strand, within BBR_RS17635 at 1735.588 kb on + strand, within BBR_RS17635 at 1735.604 kb on - strand, within BBR_RS17635 at 1735.609 kb on - strand, within BBR_RS17635 at 1735.654 kb on - strand, within BBR_RS17635 at 1735.656 kb on + strand, within BBR_RS17635 at 1735.658 kb on - strand, within BBR_RS17635 at 1735.661 kb on + strand, within BBR_RS17635 at 1735.667 kb on + strand, within BBR_RS17635 at 1735.676 kb on + strand, within BBR_RS17635 at 1735.691 kb on - strand, within BBR_RS17635 at 1735.713 kb on + strand, within BBR_RS17635 at 1735.721 kb on - strand, within BBR_RS17635 at 1735.732 kb on + strand, within BBR_RS17635 at 1735.735 kb on - strand, within BBR_RS17635 at 1735.756 kb on - strand, within BBR_RS17635 at 1735.778 kb on + strand, within BBR_RS17635 at 1735.778 kb on + strand, within BBR_RS17635 at 1735.778 kb on + strand, within BBR_RS17635 at 1735.781 kb on - strand, within BBR_RS17635 at 1735.781 kb on - strand, within BBR_RS17635 at 1735.781 kb on - strand, within BBR_RS17635 at 1735.786 kb on - strand, within BBR_RS17635 at 1735.786 kb on - strand, within BBR_RS17635 at 1735.793 kb on + strand, within BBR_RS17635 at 1735.806 kb on - strand, within BBR_RS17635 at 1735.829 kb on + strand, within BBR_RS17635 at 1735.845 kb on + strand, within BBR_RS17635 at 1735.848 kb on - strand, within BBR_RS17635 at 1735.889 kb on + strand at 1735.921 kb on - strand at 1735.921 kb on - strand at 1735.921 kb on - strand at 1735.921 kb on - strand at 1735.931 kb on + strand at 1735.980 kb on + strand at 1735.988 kb on - strand at 1736.001 kb on - strand at 1736.006 kb on - strand at 1736.068 kb on - strand at 1736.068 kb on - strand at 1736.073 kb on - strand at 1736.085 kb on + strand at 1736.090 kb on - strand at 1736.173 kb on + strand at 1736.248 kb on - strand at 1736.373 kb on - strand, within BBR_RS17640 at 1736.373 kb on - strand, within BBR_RS17640 at 1736.408 kb on + strand, within BBR_RS17640 at 1736.410 kb on + strand, within BBR_RS17640 at 1736.431 kb on + strand, within BBR_RS17640 at 1736.463 kb on + strand, within BBR_RS17640 at 1736.532 kb on + strand at 1736.533 kb on - strand at 1736.533 kb on - strand at 1736.576 kb on + strand at 1736.674 kb on + strand at 1736.679 kb on + strand at 1736.689 kb on + strand at 1736.701 kb on + strand at 1736.711 kb on - strand at 1736.744 kb on + strand at 1736.764 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v remove 1,735,225 + BBR_RS17635 0.55 +0.4 1,735,226 + BBR_RS17635 0.55 -0.2 1,735,226 + BBR_RS17635 0.55 +0.7 1,735,233 - BBR_RS17635 0.56 +1.3 1,735,233 - BBR_RS17635 0.56 -0.4 1,735,234 - BBR_RS17635 0.56 +0.7 1,735,234 - BBR_RS17635 0.56 +0.8 1,735,234 - BBR_RS17635 0.56 +0.3 1,735,236 + BBR_RS17635 0.56 +0.5 1,735,244 - BBR_RS17635 0.56 -2.3 1,735,244 - BBR_RS17635 0.56 -0.0 1,735,244 - BBR_RS17635 0.56 +0.2 1,735,252 - BBR_RS17635 0.57 -1.0 1,735,307 - BBR_RS17635 0.60 -0.5 1,735,321 - BBR_RS17635 0.60 +0.2 1,735,360 - BBR_RS17635 0.62 -0.3 1,735,360 - BBR_RS17635 0.62 +0.2 1,735,369 - BBR_RS17635 0.63 +0.0 1,735,380 - BBR_RS17635 0.64 +0.2 1,735,382 - BBR_RS17635 0.64 -0.2 1,735,397 - BBR_RS17635 0.64 +0.8 1,735,410 + BBR_RS17635 0.65 -0.0 1,735,418 - BBR_RS17635 0.66 -0.1 1,735,418 - BBR_RS17635 0.66 -0.3 1,735,456 + BBR_RS17635 0.68 -0.5 1,735,456 + BBR_RS17635 0.68 -0.6 1,735,464 - BBR_RS17635 0.68 -1.2 1,735,473 + BBR_RS17635 0.69 -0.3 1,735,504 + BBR_RS17635 0.70 -0.0 1,735,546 + BBR_RS17635 0.72 +2.1 1,735,549 - BBR_RS17635 0.73 -0.3 1,735,552 - BBR_RS17635 0.73 +1.8 1,735,557 - BBR_RS17635 0.73 +0.2 1,735,563 + BBR_RS17635 0.73 +0.1 1,735,570 + BBR_RS17635 0.74 -0.2 1,735,571 - BBR_RS17635 0.74 -1.0 1,735,578 - BBR_RS17635 0.74 +0.2 1,735,588 + BBR_RS17635 0.75 -0.1 1,735,604 - BBR_RS17635 0.76 +0.5 1,735,609 - BBR_RS17635 0.76 +0.8 1,735,654 - BBR_RS17635 0.78 -1.3 1,735,656 + BBR_RS17635 0.78 -0.4 1,735,658 - BBR_RS17635 0.78 +0.2 1,735,661 + BBR_RS17635 0.79 -1.1 1,735,667 + BBR_RS17635 0.79 +0.4 1,735,676 + BBR_RS17635 0.79 -0.1 1,735,691 - BBR_RS17635 0.80 -0.2 1,735,713 + BBR_RS17635 0.81 +0.3 1,735,721 - BBR_RS17635 0.82 +0.2 1,735,732 + BBR_RS17635 0.82 -0.0 1,735,735 - BBR_RS17635 0.83 +0.9 1,735,756 - BBR_RS17635 0.84 -0.6 1,735,778 + BBR_RS17635 0.85 -1.5 1,735,778 + BBR_RS17635 0.85 +0.9 1,735,778 + BBR_RS17635 0.85 +0.8 1,735,781 - BBR_RS17635 0.85 +0.1 1,735,781 - BBR_RS17635 0.85 -0.4 1,735,781 - BBR_RS17635 0.85 -0.8 1,735,786 - BBR_RS17635 0.85 +0.7 1,735,786 - BBR_RS17635 0.85 -0.1 1,735,793 + BBR_RS17635 0.86 -0.0 1,735,806 - BBR_RS17635 0.86 -0.1 1,735,829 + BBR_RS17635 0.88 +0.5 1,735,845 + BBR_RS17635 0.89 -0.2 1,735,848 - BBR_RS17635 0.89 -0.1 1,735,889 + -0.3 1,735,921 - +0.2 1,735,921 - -0.7 1,735,921 - +0.2 1,735,921 - +0.5 1,735,931 + +0.1 1,735,980 + +0.8 1,735,988 - -0.1 1,736,001 - -0.8 1,736,006 - -0.6 1,736,068 - +1.7 1,736,068 - -0.5 1,736,073 - +0.3 1,736,085 + -0.8 1,736,090 - -0.4 1,736,173 + -0.4 1,736,248 - -2.2 1,736,373 - BBR_RS17640 0.52 -0.4 1,736,373 - BBR_RS17640 0.52 -1.0 1,736,408 + BBR_RS17640 0.64 -2.3 1,736,410 + BBR_RS17640 0.65 -0.3 1,736,431 + BBR_RS17640 0.72 -1.0 1,736,463 + BBR_RS17640 0.84 -1.2 1,736,532 + -0.6 1,736,533 - -0.2 1,736,533 - +0.2 1,736,576 + +0.3 1,736,674 + -0.6 1,736,679 + -0.1 1,736,689 + +0.5 1,736,701 + -0.8 1,736,711 - +0.4 1,736,744 + +0.5 1,736,764 - +0.6
Or see this region's nucleotide sequence