Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS17510

Experiment: reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	reconstituted MRS with Bioecolians-prebiotic 0.5 %w/v
1,706,045	+		BBR_RS17505	0.13	-0.6
1,706,053	-		BBR_RS17505	0.13	-0.0
1,706,059	-		BBR_RS17505	0.14	-0.8
1,706,075	-		BBR_RS17505	0.15	-1.7
1,706,112	+		BBR_RS17505	0.19	-1.0
1,706,129	+		BBR_RS17505	0.21	-0.6
1,706,138	-		BBR_RS17505	0.21	+0.1
1,706,154	+		BBR_RS17505	0.23	-1.7
1,706,200	+		BBR_RS17505	0.27	+0.5
1,706,202	+		BBR_RS17505	0.27	-1.8
1,706,212	+		BBR_RS17505	0.28	-1.4
1,706,212	+		BBR_RS17505	0.28	-1.1
1,706,220	-		BBR_RS17505	0.29	-0.3
1,706,227	+		BBR_RS17505	0.30	-0.8
1,706,232	+		BBR_RS17505	0.30	-1.0
1,706,237	+		BBR_RS17505	0.31	+0.3
1,706,244	-		BBR_RS17505	0.31	-1.4
1,706,263	-		BBR_RS17505	0.33	-2.7
1,706,337	+		BBR_RS17505	0.40	-2.9
1,706,345	-		BBR_RS17505	0.41	-0.9
1,706,357	-		BBR_RS17505	0.42	-0.7
1,706,397	+		BBR_RS17505	0.46	-1.4
1,706,399	+		BBR_RS17505	0.46	-0.9
1,706,399	+		BBR_RS17505	0.46	-1.0
1,706,399	+		BBR_RS17505	0.46	+0.8
1,706,399	+		BBR_RS17505	0.46	-1.3
1,706,399	+		BBR_RS17505	0.46	-1.5
1,706,405	-		BBR_RS17505	0.47	-1.8
1,706,407	-		BBR_RS17505	0.47	-0.4
1,706,407	-		BBR_RS17505	0.47	-0.7
1,706,407	-		BBR_RS17505	0.47	-1.0
1,706,435	+		BBR_RS17505	0.49	-1.5
1,706,446	+		BBR_RS17505	0.51	-1.5
1,706,452	-		BBR_RS17505	0.51	-0.9
1,706,470	+		BBR_RS17505	0.53	-0.3
1,706,470	+		BBR_RS17505	0.53	-0.6
1,706,471	+		BBR_RS17505	0.53	-1.9
1,706,478	-		BBR_RS17505	0.54	-1.5
1,706,481	+		BBR_RS17505	0.54	-1.3
1,706,489	+		BBR_RS17505	0.55	-3.0
1,706,528	-		BBR_RS17505	0.58	-0.5
1,706,528	-		BBR_RS17505	0.58	-0.9
1,706,531	-		BBR_RS17505	0.59	+0.3
1,706,531	-		BBR_RS17505	0.59	-0.9
1,706,546	-		BBR_RS17505	0.60	-0.5
1,706,601	+		BBR_RS17505	0.65	-1.2
1,706,618	-		BBR_RS17505	0.67	-0.4
1,706,677	-		BBR_RS17505	0.72	-1.1
1,706,703	+		BBR_RS17505	0.75	-1.8
1,706,703	+		BBR_RS17505	0.75	+1.3
1,706,713	-		BBR_RS17505	0.76	-0.7
1,706,736	+		BBR_RS17505	0.78	-0.5
1,706,736	+		BBR_RS17505	0.78	-0.6
1,706,772	+		BBR_RS17505	0.81	-1.2
1,706,828	-		BBR_RS17505	0.87	-0.9
1,706,838	-		BBR_RS17505	0.88	-1.3
1,706,842	+		BBR_RS17505	0.88	-1.8
1,706,858	-		BBR_RS17505	0.89	-0.9
1,706,869	+				-1.2
1,706,893	+				-0.8
1,706,901	-				-1.5
1,706,901	-				-2.6
1,706,901	-				-2.3
1,706,903	+				-1.3
1,706,905	+				-1.2
1,706,948	+				-0.6
1,706,949	+				-1.3
1,706,950	+				-0.9
1,706,950	+				-3.4
1,706,990	+				-0.1
1,706,998	-				-2.4
1,707,002	+				-1.0
1,707,038	-				-2.4
1,707,163	-		BBR_RS17510	0.19	-1.3
1,707,183	+		BBR_RS17510	0.21	-2.7
1,707,201	+		BBR_RS17510	0.23	-1.5
1,707,206	+		BBR_RS17510	0.23	-0.3
1,707,209	-		BBR_RS17510	0.23	-1.3
1,707,236	-		BBR_RS17510	0.26	-1.4
1,707,243	+		BBR_RS17510	0.27	-1.1
1,707,269	+		BBR_RS17510	0.29	-0.3
1,707,298	+		BBR_RS17510	0.32	-1.8
1,707,365	+		BBR_RS17510	0.38	-1.5
1,707,365	+		BBR_RS17510	0.38	-0.1
1,707,383	-		BBR_RS17510	0.40	-0.7
1,707,445	-		BBR_RS17510	0.46	-2.0
1,707,445	-		BBR_RS17510	0.46	-0.2
1,707,446	+		BBR_RS17510	0.46	-2.2
1,707,447	+		BBR_RS17510	0.46	-0.6
1,707,476	-		BBR_RS17510	0.49	-1.3
1,707,537	+		BBR_RS17510	0.55	-1.6
1,707,547	+		BBR_RS17510	0.56	-1.0
1,707,581	-		BBR_RS17510	0.59	-0.5
1,707,619	+		BBR_RS17510	0.63	-1.2
1,707,708	+		BBR_RS17510	0.71	-1.5
1,707,718	+		BBR_RS17510	0.72	-1.6
1,707,774	+		BBR_RS17510	0.78	-4.2
1,707,784	-		BBR_RS17510	0.79	-2.2
1,707,784	-		BBR_RS17510	0.79	-1.3
1,707,804	+		BBR_RS17510	0.81	-1.6
1,707,815	+		BBR_RS17510	0.82	-1.0
1,707,882	-		BBR_RS17510	0.88	-1.3
1,707,907	+				-0.2
1,707,912	+				-2.5
1,707,914	+				-3.1
1,707,917	-				+0.0
1,707,919	+				-1.0
1,707,919	+				-1.1
1,707,919	+				-1.3
1,707,920	-				-2.6
1,707,927	-				+0.2
1,707,997	+				-0.5
1,708,007	+				-0.4
1,708,007	+				-0.7
1,708,017	-				-1.3
1,708,017	-				-2.0
1,708,103	+		BBR_RS17515	0.18	-1.2
1,708,103	+		BBR_RS17515	0.18	-0.9
1,708,103	+		BBR_RS17515	0.18	-1.4
1,708,103	+		BBR_RS17515	0.18	-1.4
1,708,111	-		BBR_RS17515	0.19	-1.7
1,708,111	-		BBR_RS17515	0.19	-0.6
1,708,169	-		BBR_RS17515	0.29	+1.1
1,708,203	-		BBR_RS17515	0.35	-1.1
1,708,213	-		BBR_RS17515	0.37	-0.5
1,708,213	-		BBR_RS17515	0.37	-1.2
1,708,242	+		BBR_RS17515	0.42	-1.3
1,708,242	+		BBR_RS17515	0.42	+0.1
1,708,242	+		BBR_RS17515	0.42	-1.7
1,708,310	+		BBR_RS17515	0.54	+0.2
1,708,310	+		BBR_RS17515	0.54	-1.2
1,708,318	-		BBR_RS17515	0.55	-1.4
1,708,318	-		BBR_RS17515	0.55	+0.0
1,708,331	+		BBR_RS17515	0.58	-0.6
1,708,341	+		BBR_RS17515	0.60	-1.4
1,708,369	+		BBR_RS17515	0.64	-1.1
1,708,377	-		BBR_RS17515	0.66	-0.3
1,708,377	-		BBR_RS17515	0.66	-0.3
1,708,377	-		BBR_RS17515	0.66	-0.0
1,708,377	-		BBR_RS17515	0.66	-0.7
1,708,382	-		BBR_RS17515	0.67	-0.8
1,708,392	-		BBR_RS17515	0.68	-0.9
1,708,418	-		BBR_RS17515	0.73	-0.9
1,708,484	-		BBR_RS17515	0.84	+0.3
1,708,564	-				-1.2
1,708,564	-				-0.0
1,708,577	-				+0.2
1,708,612	+				-0.2
1,708,612	+				+0.2
1,708,645	-				-0.3
1,708,654	-				+0.3
1,708,660	+				+0.3
1,708,683	-				+0.7
1,708,684	-				+0.1
1,708,691	-				+0.3
1,708,708	+				+0.0
1,708,718	+				+0.5
1,708,727	+				-0.8
1,708,800	-		BBR_RS17520	0.33	+0.2
1,708,810	-		BBR_RS17520	0.36	-1.7
1,708,810	-		BBR_RS17520	0.36	+0.4
1,708,827	+		BBR_RS17520	0.41	+0.2
1,708,827	+		BBR_RS17520	0.41	-0.1
1,708,836	-		BBR_RS17520	0.44	-0.8
1,708,883	+		BBR_RS17520	0.60	+0.5
1,708,893	+		BBR_RS17520	0.63	-0.6
1,708,962	-		BBR_RS17520	0.85	+0.4
1,708,963	+		BBR_RS17520	0.85	+0.6
1,708,975	-		BBR_RS17520	0.89	-1.0
1,708,998	-				+0.9